Complete Genome Sequence of Gallibacterium anatis Strain UMN179, Isolated from a Laying Hen with Peritonitis

Johnson, Timothy J.; Fernández-Alarcón, Claudia; Bojesen, Anders Miki; Nolan, Lisa K.; Trampel, Darrell W.; Seemann, Torsten

doi:10.1128/jb.05177-11

Cited by 24 publications

(15 citation statements)

References 16 publications

(10 reference statements)

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“…Environmental reservoirs of antibiotic resistance can develop in farmland (van den Bogaard and Stobberingh, ; Johnston and Jaykus, ) since food and animal Enterococcus species can efficiently transfer antibiotic resistance to human strains in inter‐specific matings (van den Bogaard and Stobberingh, ; Zhang et al., ). Consistent with the hypothesis that DNSE isolates may develop in animal Enterococcus species, we found nucleotide mutations in DNSE isolates (Humphries et al., ) in genes that have also been described in many bacteria found in poultry such as putative lipoprotein (Johnson et al., ), regulatory protein spx (Voget et al., ), protein DNAB (Lowder et al., ). Thus, a possible mechanism of resistance in DNSE could be transfer of antibiotic resistance genes encountered in animal products (Kelesidis et al., ).…”

Section: Farm Animals May Be a Source For Development Of De Novo Dnsesupporting

confidence: 89%

“…However, the mechanisms of development of DNSE among patients without prior exposure to daptomycin (de novo DNSE) remain to be determined (Kelesidis et al, 2012b). (Humphries et al, 2012) in genes that have also been described in many bacteria found in poultry such as putative lipoprotein (Johnson et al, 2011), regulatory protein spx (Voget et al, 2011), protein DNAB (Lowder et al, 2009). Thus, a possible mechanism of resistance in DNSE could be transfer of antibiotic resistance genes encountered in animal products (Kelesidis et al, 2012b).…”

Section: Acquired Daptomycin Resistance Among Enterococcus Spp May Bmentioning

confidence: 99%

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The Zoonotic Potential of Daptomycin Non‐susceptible Enterococci

Kelesidis

2013

Zoonoses and Public Health

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Impacts• Daptomycin non-susceptible enterococcus (DNSE) is an emerging clinical problem.• Determining risk factors associated with de novo DNSE infections will aid in understanding the mechanisms of daptomycin non-susceptibility.• Humans in contact with animals are at risk of carriage of DNSE, and this manuscript reviews the scientific evidence regarding the zoonotic potential of daptomycin non-susceptible enterococci. SummaryDaptomycin non-susceptible Enterococcus (DNSE) is an emerging clinical problem. Little is known about how de novo DNSE infections develop or the risk factors associated with them. Determining risk factors associated with de novo DNSE infections will aid in understanding the mechanisms of daptomycin nonsusceptibility. Humans in contact with animals worldwide are at risk of carriage of multidrug-resistant bacteria. Herein, I review the scientific evidence that supports the hypothesis that transport of daptomycin non-susceptibility genes between animals and humans may be a possible mechanism for development of de novo daptomycin non-susceptibility in enterococci.

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Section: Farm Animals May Be a Source For Development Of De Novo Dnsesupporting

confidence: 89%

Section: Acquired Daptomycin Resistance Among Enterococcus Spp May Bmentioning

confidence: 99%

The Zoonotic Potential of Daptomycin Non‐susceptible Enterococci

Kelesidis

2013

Zoonoses and Public Health

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“…The same competence genes were identified in the published genome sequence of G. anatis UMN179 (18) and in the provisional genome sequence of another G. anatis strain, F149…”

Section: Identification Of a Putative Competence Regulon In G Anatismentioning

confidence: 53%

Natural Transformation of Gallibacterium anatis

Kristensen

Sinha

Boyce

et al. 2012

Appl Environ Microbiol

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ABSTRACTGallibacterium anatisis a pathogen of poultry. Very little is known about its genetics and pathogenesis. To enable the study of gene function inG. anatis, we have established methods for transformation and targeted mutagenesis. The genusGallibacteriumbelongs to thePasteurellaceae, a group with several naturally transformable members, includingHaemophilus influenzae. Bioinformatics analysis identifiedG. anatishomologs of theH. influenzaecompetence genes, and natural competence was induced inG. anatisby the procedure established forH. influenzae: transfer from rich medium to the starvation medium M-IV. This procedure gave reproducibly high transformation frequencies withG. anatischromosomal DNA and with linearized plasmid DNA carryingG. anatissequences. Both DNA types integrated into theG. anatischromosome by homologous recombination. Targeted mutagenesis gave transformation frequencies of >2 × 10−4transformants CFU−1. Transformation was also efficient with circular plasmid containing noG. anatisDNA; this resulted in the establishment of a self-replicating plasmid. Nine diverseG. anatisstrains were found to be naturally transformable by this procedure, suggesting that natural competence is common and the M-IV transformation procedure widely applicable for this species. TheG. anatisgenome is only slightly enriched for the uptake signal sequences identified in other pasteurellaceaen genomes, butG. anatisdid preferentially take up its own DNA over that ofEscherichia coli. Transformation by electroporation was not effective for chromosomal integration but could be used to introduce self-replicating plasmids. The findings described here provide important tools for the genetic manipulation ofG. anatis.

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“…Strain F149 T is the type strain for G. anatis and was isolated from a healthy duck in Denmark in 1979 [3]. Strain UMN179 was isolated in 2007 from a commercial laying hen with peritonitis in Iowa, USA [14]. Additional isolates for MLST analysis were obtained from two commercial egg laying companies in Iowa, USA in 2006 and 2007 involving laying hens from four farm systems and eleven different farms (Table 1).…”

Section: Methodsmentioning

confidence: 99%

“…It has also been proposed that G. anatis isolates vary in their virulence potential [11], [12], and amplified fragment length polymorphism (AFLP) has revealed that there is substantial genetic diversity among the Gallibacterium isolates dominating among and between successive flocks [13]. The purpose of this study was to generate and compare the genome sequences of virulent and avirulent G. anatis isolates to better understand their genetic composition, and to develop a multilocus sequence typing (MLST) procedure for assessing the genetic relatedness of G. anatis isolates and their genomic content [14].…”

Section: Introductionmentioning

confidence: 99%

Genome Analysis and Phylogenetic Relatedness of Gallibacterium anatis Strains from Poultry

et al. 2013

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Peritonitis is the major disease problem of laying hens in commercial table egg and parent stock operations. Despite its importance, the etiology and pathogenesis of this disease have not been completely clarified. Although avian pathogenic Escherichia coli (APEC) isolates have been incriminated as the causative agent of laying hen peritonitis, Gallibacterium anatis are frequently isolated from peritonitis lesions. Despite recent studies suggesting a role for G. anatis in the pathogenesis of peritonitis, little is known about the organism’s virulence mechanisms, genomic composition and population dynamics. Here, we compared the genome sequences of three G. anatis isolates in an effort to understand its virulence mechanisms and identify novel antigenic traits. A multilocus sequence typing method was also established for G. anatis and used to characterize the genotypic relatedness of 71 isolates from commercial laying hens in Iowa and 18 international reference isolates. Genomic comparisons suggest that G. anatis is a highly diverse bacterial species, with some strains possessing previously described and potential virulence factors, but with a core genome containing several antigenic candidates. Multilocus sequence typing effectively distinguished 82 sequence types and several clonal complexes of G. anatis, and some clones seemed to predominate among G. anatis populations from commercial layers in Iowa. Biofilm formation and resistance to antimicrobial agents was also observed in several clades. Overall, the genomic diversity of G. anatis suggests that multiple lineages exist with differing pathogenic potential towards birds.

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Complete Genome Sequence of Gallibacterium anatis Strain UMN179, Isolated from a Laying Hen with Peritonitis

Cited by 24 publications

References 16 publications

The Zoonotic Potential of Daptomycin Non‐susceptible Enterococci

The Zoonotic Potential of Daptomycin Non‐susceptible Enterococci

Natural Transformation of Gallibacterium anatis

Genome Analysis and Phylogenetic Relatedness of Gallibacterium anatis Strains from Poultry

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