Complete Genome Sequence of Flavobacterium psychrophilum Strain CSF259-93, Used To Select Rainbow Trout for Increased Genetic Resistance against Bacterial Cold Water Disease

Wiens, Gregory D.; LaPatra, Scott E.; Welch, Timothy J.; Rexroad, Caird E.; Call, Douglas R.; Cain, Kenneth D.; LaFrentz, Benjamin R.; Vaisvil, Benjamin; Schmitt, Daniel P.; Kapatral, Vinayak

doi:10.1128/genomea.00889-14

Cited by 25 publications

(24 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bacterial challenge was carried out in the NCCCWA challenge facility with F. psychrophilum strain CSF-259-93 (initially provided by Dr. S. LaPatra, Clear Springs Foods, Inc.). This strain was previously isolated from a BCWD field-case and maintained at −80°C in TYES media supplemented with 10% (v/v) glycerol and has been consistently utilized as the challenge strain within the selective breeding program (Hadidi et al, 2008 ; Silverstein et al, 2009 ; Leeds et al, 2010 ; Wiens et al, 2013a ) and the complete genome sequence determined (Wiens et al, 2014 ). Frozen stock was cultivated on TYES media for 5 days at 15°C, suspended in PBS and O.D.…”

Section: Methodsmentioning

confidence: 99%

Whole-body transcriptome of selectively bred, resistant-, control-, and susceptible-line rainbow trout following experimental challenge with Flavobacterium psychrophilum

et al. 2015

Self Cite

View full text Add to dashboard Cite

Genetic improvement for enhanced disease resistance in fish is an increasingly utilized approach to mitigate endemic infectious disease in aquaculture. In domesticated salmonid populations, large phenotypic variation in disease resistance has been identified but the genetic basis for altered responsiveness remains unclear. We previously reported three generations of selection and phenotypic validation of a bacterial cold water disease (BCWD) resistant line of rainbow trout, designated ARS-Fp-R. This line has higher survival after infection by either standardized laboratory challenge or natural challenge as compared to two reference lines, designated ARS-Fp-C (control) and ARS-Fp-S (susceptible). In this study, we utilized 1.1 g fry from the three genetic lines and performed RNA-seq to measure transcript abundance from the whole body of naive and Flavobacterium psychrophilum infected fish at day 1 (early time-point) and at day 5 post-challenge (onset of mortality). Sequences from 24 libraries were mapped onto the rainbow trout genome reference transcriptome of 46,585 predicted protein coding mRNAs that included 2633 putative immune-relevant gene transcripts. A total of 1884 genes (4.0% genome) exhibited differential transcript abundance between infected and mock-challenged fish (FDR < 0.05) that included chemokines, complement components, tnf receptor superfamily members, interleukins, nod-like receptor family members, and genes involved in metabolism and wound healing. The largest number of differentially expressed genes occurred on day 5 post-infection between naive and challenged ARS-Fp-S line fish correlating with high bacterial load. After excluding the effect of infection, we identified 21 differentially expressed genes between the three genetic lines. In summary, these data indicate global transcriptome differences between genetic lines of naive animals as well as differentially regulated transcriptional responses to infection.

show abstract

Section: Methodsmentioning

confidence: 99%

Whole-body transcriptome of selectively bred, resistant-, control-, and susceptible-line rainbow trout following experimental challenge with Flavobacterium psychrophilum

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…Water quality parameters at the NCCCWA have been described previously [38]. Fish were challenged by intraperitoneal injection with 500 L of PBS alone, or PBS containing 2.3 ± 0.4 x 10 8 CFU of the genomesequenced strain of F. psychrophilum CSF259-93 [40]. During the experimental challenge period, one ARS-Fp-S line fish died on day 4 and Fp was confirmed by plate culture using TYES media.…”

Section: Samplingmentioning

confidence: 99%

Proinflammatory cytokine and cytokine receptor gene expression kinetics following challenge with Flavobacterium psychrophilum in resistant and susceptible lines of rainbow trout (Oncorhynchus mykiss)

Kutyrev

Cleveland

Leeds

et al. 2016

Fish & Shellfish Immunology

Self Cite

View full text Add to dashboard Cite

Flavobacterium psychrophilum (Fp) is the causative agent of bacterial cold water disease (BCWD) which causes appreciable economic losses in rainbow trout aquaculture. We previously reported development of a genetic line, designated ARS-Fp-R that exhibits higher survival relative to a susceptible line, designated ARS-Fp-S, following either laboratory or natural on-farm challenge. The objectives of this study were to determine the temporal kinetics of gene expression between experimentally-challenged ARS-Fp-R and ARS-Fp-S fish and the correlation between gene expression and pathogen load. We developed a GeXP multiplex RT-PCR assay to simultaneously examine expression of immune-relevant genes, concentrating on tumor necrosis factor and interleukin-1 ligand/receptor systems and acute phase response genes. Spleen tissue was sampled at 6 h, 24 h, 48 h and 144 h post-challenge and pathogen load quantified by qPCR. Transcript abundance of cytokine genes tnfa1, tnfa2, tnfa3, il1b1, il1b2, il11a; acute phase response genes saa and drtp1; and putative cytokine receptors il1r1-like-b, il1r2, tnfrsf1a, tnfrsf9, tnfrsf1a-like-b increased following challenge while the transcript abundance of il1r-like-1 and tnfrsf1a-like-a decreased compared to PBS-injected line-matched control fish. Principal component analysis identified transcript levels of genes il1r-like-1 and tnfrsf1a-like-a as exhibiting differential expression between genetic lines. In summary, Fp i.p. injection challenge elicited a proinflammatory cytokine gene expression response in the spleen, with ARS-Fp-R line fish exhibiting modestly higher basal expression levels of several putative cytokine receptors. This study furthers the understanding of the immune response following Fp challenge and differences in gene expression associated with selective breeding for disease resistance.

show abstract

“…BCWD has a significant economic impact on aquaculture operations in the United States and Canada. The genome sequences of the F. psychrophilum vaccine strain ( 5 ), type strain ( 6 ), and a European isolate are available ( 7 ). Here, we report the sequencing of three BCWD outbreak-associated F. psychrophilum strains isolated from rainbow trout ( Oncorhynchus mykiss ) from three different hatcheries in the United States.…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequences of Three Flavobacterium psychrophilum Strains Isolated from Coldwater Disease Outbreaks at Three Production Hatcheries

et al. 2016

View full text Add to dashboard Cite

show abstract

Complete Genome Sequence of Flavobacterium psychrophilum Strain CSF259-93, Used To Select Rainbow Trout for Increased Genetic Resistance against Bacterial Cold Water Disease

Cited by 25 publications

References 21 publications

Whole-body transcriptome of selectively bred, resistant-, control-, and susceptible-line rainbow trout following experimental challenge with Flavobacterium psychrophilum

Whole-body transcriptome of selectively bred, resistant-, control-, and susceptible-line rainbow trout following experimental challenge with Flavobacterium psychrophilum

Proinflammatory cytokine and cytokine receptor gene expression kinetics following challenge with Flavobacterium psychrophilum in resistant and susceptible lines of rainbow trout (Oncorhynchus mykiss)

Draft Genome Sequences of Three Flavobacterium psychrophilum Strains Isolated from Coldwater Disease Outbreaks at Three Production Hatcheries

Contact Info

Product

Resources

About