Complete Genome Sequence of Escherichia coli Myophage Mangalitsa

Atkison, Cameron; Boeckman, Justin; Newkirk, Heather; Liu, Mei; Gill, Jason J.; Cahill, Jesse; Ramsey, Jolene

doi:10.1128/mra.01045-19

Cited by 4 publications

(3 citation statements)

References 19 publications

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“…The φBO1E phage belongs to the Podoviridae family and is a member of the recently described Drulisvirus genus [ 27 ]. Even if the information on the infection specificity of Drulisvirus phages is currently available in a limited number of cases, i.e., KpV41, KpV48, KpV71, KpV74, KpV475 [ 28 ], myPSH1235 [ 29 ], πVLC1-4 [ 30 ] and vB_KpnP_IME337 [ 31 ], for most of these phages a perfect correlation between the bacterial cps type and phage susceptibility has been demonstrated [ 10 , 28 , 30 , 31 ] while this information is lacking or a broader specificity has been determined in a limited number of studies [ 32 , 33 , 34 , 35 , 36 , 37 , 38 ] ( Table S2 ). This is in line with our results and, together with the observation that these phages possess a pectate lyase domain in their terminal fibers, suggests that this genus is characterized by the ability of targeting bacterial polysaccharides for the early infection steps.…”

Section: Discussionmentioning

confidence: 99%

Phage Resistance Is Associated with Decreased Virulence in KPC-Producing Klebsiella pneumoniae of the Clonal Group 258 Clade II Lineage

et al. 2021

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Phage therapy is now reconsidered with interest in the treatment of bacterial infections. A major piece of information for this application is the definition of the molecular targets exploited by phages to infect bacteria. Here, the genetic basis of resistance to the lytic phage φBO1E by its susceptible host Klebsiella pneumoniae KKBO-1 has been investigated. KKBO-1 phage-resistant mutants were obtained by infection at high multiplicity. One mutant, designated BO-FR-1, was selected for subsequent experiments, including virulence assessment in a Galleria mellonella infection model and characterization by whole-genome sequencing. Infection with BO-FR-1 was associated with a significantly lower mortality when compared to that of the parental strain. The BO-FR-1 genome differed from KKBO-1 by a single nonsense mutation into the wbaP gene, which encodes a glycosyltransferase involved in the first step of the biosynthesis of the capsular polysaccharide (CPS). Phage susceptibility was restored when BO-FR-1 was complemented with the constitutive wbaP gene. Our results demonstrated that φBO1E infects KKBO-1 targeting the bacterial CPS. Interestingly, BO-FR-1 was less virulent than the parental strain, suggesting that in the context of the interplay among phage, bacterial pathogen and host, the emergence of phage resistance may be beneficial for the host.

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Section: Discussionmentioning

confidence: 99%

Phage Resistance Is Associated with Decreased Virulence in KPC-Producing Klebsiella pneumoniae of the Clonal Group 258 Clade II Lineage

et al. 2021

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“…This heat map identi ed both SKA49 and SKA64 as new species. SKA49 proteins have high homology with three phages Mangalitsa (27%), ST32 (26%) and GJ1 (15.1%), however only ST32 is physically characterized and for remaining two only genomes have been reported (19,20). For SKA64 same is true as genomes of few closely related homologs have been reported but not complete characterization (21,22).…”

Section: Phylogenetic Analysis Of Ska49 and Ska64mentioning

confidence: 98%

Two Novel Lytic Bacteriophages having Therapeutic Potential against MDR Avian Pathogenic Escherichia coli strains of zoonotic potential

Sattar

Bailie

Yaqoob

et al. 2023

Preprint

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Avian pathogenic E. coli (APEC) is associated with local and systemic infections in poultry, ducks turkeys and many other avian species which leads to heavy economical losses. These APEC strains are presumed to possess zoonotic potential due to common virulence markers that can cause urinary tract infections in humans. The prophylactic use of antibiotics in poultry sector has led to rapid emergence of Multiple Drug Resistant (MDR) APEC strains that also put human populations at risk acting as reservoirs. This calls for consideration of alternative strategies for their treatment such as phage therapy. Here, we report isolation, characterization, and genome analysis of two novel lytic phage species (Escherichia phage SKA49 and Escherichia phage SKA64) against MDR strain of APEC, QZJM25. Both phages were able to keep QZJM25 growth significantly less than bacterial control for approximately 18 hours. Host range was tested against Escherichia coli strains of poultry and human UTI infections. SKA49 had broader host range contrast to SKA64. Both phages were stable at 37°C only. Their genome analysis indicated their safety as no recombination, integration and host virulence genes were identified. Based on this analysis both these phages can be used as effective biocontrol agents in poultry industry.

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“…This feature and the presence of all Myduc genes on the top strand suggest that phage Myduc may eject its DNA in a manner similar to that of phage T7, which is assisted by the polymerase’s transcriptional activity. Myduc shares similar genome features and shares at least 44 of its 79 total proteins (BLASTp; E value, <0.001) with a group of small myophages (52,000 to 56,000 bp in genome size) that includes Enterobacteria phage phiEcoM-GJ1 (GenBank accession number EF460875) (19), Escherichia phages Mangalitsa (GenBank accession number MN045229) (20) and ST32 (GenBank accession number MF044458) (21), and Pectobacterium phages PP101 (GenBank accession number KY087898) and PM1 (GenBank accession number KF534715) (22).…”

Section: Announcementmentioning

confidence: 99%