Complete Genome Sequence of Escherichia coli Strain FEX669, a ColV Plasmid-Containing Isolate from Retail Chicken Meat

Abstract

Escherichia coli strain FEX669 was isolated from retail ground chicken and shown to contain the extraintestinal pathogenic E. coli (ExPEC) virulence genes sfaD, focC, and iutA. Because this presumptive ExPEC strain was isolated from a retail food item and it was a weak biofilm former, it was characterized using whole-genome sequencing using the PacBio RS II platform. Genomic analysis showed that the FEX669 chromosome is 4,973,943 bp long, with a GC content of 50.47%, and is accompanied by a ColV plasmid that is 237,102 bp long, with a GC content of 50.49%.

Whole-genome sequencing and characterization of Escherichia coli human isolate DP033

“…For genome sequencing, genomic DNA was isolated and sequenced as described previously ( 5 ) with 448× coverage. Default parameters were used for all software analysis unless otherwise specified.…”

“…To date, multiple chicken-source putative ExPEC strains have been characterized by whole-genome sequencing ( 5 , 16 , 17 ). The resulting sequence data can form a database, offering the possibility of determining the relatedness of strains from food or food animals and humans through in silico genome typing and comparative genomics ( 13 ).…”