Complete genome sequence of endophyte Bacillus flexus KLBMP 4941 reveals its plant growth promotion mechanism and genetic basis for salt tolerance

Wang, Tiantian; Ding, Peng; Chen, Pan; Xing, Ke; Bai, Juan-Luan; Wan, Wen; Jiang, Ji-Hong; Qin, Sheng

doi:10.1016/j.jbiotec.2017.09.001

Cited by 38 publications

(13 citation statements)

References 19 publications

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“…Complete genome information of endophyte Bacillus flexus KLBMP 4941; Pseudomonas azotoformans S4, P. Antarctica PAMC 27494, P. putida BIRD-1 and P. aurantiaca strain JD37 contains genes related to plant growth promotion, biocontrol and salt tolerance [ 30 , 32 , 35 , 66 ]. In agreement with those observations, we also found genes that are responsible for plant growth promotion and abiotic stress tolerance the complete genome of Pseudomonas sp.…”

Section: Discussionmentioning

confidence: 99%

Induced Salt Tolerance of Perennial Ryegrass by a Novel Bacterium Strain from the Rhizosphere of a Desert Shrub Haloxylon ammodendron

Niu

Zhao

et al. 2018

IJMS

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Drought and soil salinity reduce agricultural output worldwide. Plant-growth-promoting rhizobacteria (PGPR) can enhance plant growth and augment plant tolerance to biotic and abiotic stresses. Haloxylon ammodendron, a C4 perennial succulent xerohalophyte shrub with excellent drought and salt tolerance, is naturally distributed in the desert area of northwest China. In our previous work, a bacterium strain numbered as M30-35 was isolated from the rhizosphere of H. ammodendron in Tengger desert, Gansu province, northwest China. In current work, the effects of M30-35 inoculation on salt tolerance of perennial ryegrass were evaluated and its genome was sequenced to identify genes associated with plant growth promotion. Results showed that M30-35 significantly enhanced growth and salt tolerance of perennial ryegrass by increasing shoot fresh and dry weights, chlorophyll content, root volume, root activity, leaf catalase activity, soluble sugar and proline contents that contributed to reduced osmotic potential, tissue K+ content and K+/Na+ ratio, while decreasing malondialdehyde (MDA) content and relative electric conductivity (REC), especially under higher salinity. The genome of M30-35 contains 4421 protein encoding genes, 12 rRNA, 63 tRNA-encoding genes and four rRNA operons. M30-35 was initially classified as a new species in Pseudomonas and named as Pseudomonas sp. M30-35. Thirty-four genes showing homology to genes associated with PGPR traits and abiotic stress tolerance were identified in Pseudomonas sp. M30-35 genome, including 12 related to insoluble phosphorus solubilization, four to auxin biosynthesis, four to other process of growth promotion, seven to oxidative stress alleviation, four to salt and drought tolerance and three to cold and heat tolerance. Further study is needed to clarify the correlation between these genes from M30-35 and the salt stress alleviation of inoculated plants under salt stress. Overall, our research indicated that desert shrubs appear rich in PGPRs that can help important crops tolerate abiotic stress.

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Section: Discussionmentioning

confidence: 99%

Induced Salt Tolerance of Perennial Ryegrass by a Novel Bacterium Strain from the Rhizosphere of a Desert Shrub Haloxylon ammodendron

Niu

Zhao

et al. 2018

IJMS

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“…EB can enhance Arabidopsis thaliana growth under salt stress and can tolerate high salt concentrations and show a potential competitive advantage of surviving in saline soils [18,19]. The complete genome sequence of the endophyte Bacillus flexus helps in revealing the plant growth-promoting mechanism of EB [20]. However, limited studies have focused on the EB genes involved in salt stress response.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of Ice Plant Growth-Promoting Endophytic Bacterium Halomonas sp. Strain MC1 to Identify the Genes Involved in Salt Tolerance

et al. 2020

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Salt stress is an important adverse condition encountered during plant and microbe growth in terrestrial soil ecosystems. Currently, how ice plant (Mesembryanthemum crystallinum) growth-promoting endophytic bacteria (EB) cope with salt stress and regulate growth and the genes responsible for salt tolerance remain unknown. We applied RNA-Seq technology to determine the growth mechanism of the EB Halomonas sp. MC1 strain and the genes involved in salt tolerance. A total of 893 genes were significantly regulated after salt treatment. These genes included 401 upregulated and 492 downregulated genes. Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes analysis revealed that the most enriched genes included those related to the outer membrane-bounded periplasmic space, ATPase activity, catabolic process, and proton transmembrane transport. The quantitative real-time polymerase chain reaction data were similar to those obtained from RNA-Seq. The MC1 strain maintained survival under salt stress by regulating cellular and metabolic processes and pyruvate metabolism pathways such as organic and carboxylic acid catabolic pathways. We highlighted the response mechanism of Halomonas sp. MC1 to fully understand the dynamics of complex salt–microbe interactions.

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“…Generally, internal parts of the plant provide homogeneous and sound environment to microbes in contrast to rhizosphere and phylloplane, where microbes struggle for their food and undergo drastic temperature shift and ultra violet (UV) revelation [4]. Several studies reported mammoth diversity of endophytic bacteria associated with plant system and identified their prospective role in plant growth promotion through multiple mechanisms, including rapid synthesis of growth hormones, siderophores, hydrolytic enzymes, secondary metabolites, and activation of induced plant resistance (IPR) [5][6][7][8][9]. Emerging research evidences clearly pointed out the significance of microbiota dwelling inside the pearl millet host in offering stress tolerance as well as assistance in enhancing the survival and fitness attributes under unfavorable conditions [3,8].…”

Section: Introductionmentioning

confidence: 99%

Plant growth promoting and antifungal activity in endophytic Bacillus strains from pearl millet (Pennisetum glaucum)

et al. 2019

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Bacterial endophytes are well known inhabitants of living plant system and perform important assignments in maintaining plant growth and health. Currently, limited reports are available on the endophytes of pearl millet (Pennisetum glaucum) reflecting antagonistic and plant growth promoting (PGP) attributes. Therefore, the major objectives of current investigation were to identify antagonistic strains of endophytic Bacillus from pearl millet and further illustrate their PGP capabilities. In this study, 19 endophytic Bacillus strains (EPP5,

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Complete genome sequence of endophyte Bacillus flexus KLBMP 4941 reveals its plant growth promotion mechanism and genetic basis for salt tolerance

Cited by 38 publications

References 19 publications

Induced Salt Tolerance of Perennial Ryegrass by a Novel Bacterium Strain from the Rhizosphere of a Desert Shrub Haloxylon ammodendron

Induced Salt Tolerance of Perennial Ryegrass by a Novel Bacterium Strain from the Rhizosphere of a Desert Shrub Haloxylon ammodendron

Transcriptome Analysis of Ice Plant Growth-Promoting Endophytic Bacterium Halomonas sp. Strain MC1 to Identify the Genes Involved in Salt Tolerance

Plant growth promoting and antifungal activity in endophytic Bacillus strains from pearl millet (Pennisetum glaucum)

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