Complete Genome Sequence of Elizabethkingia meningoseptica, Isolated from a T-Cell Non-Hodgkin's Lymphoma Patient

Sun, Guogui; Wang, Lei; Bao, Celimuge; Li, Tiansheng; Ma, Lili; Chen, Li

doi:10.1128/genomea.00673-15

Cited by 17 publications

(14 citation statements)

References 7 publications

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“…E. meningoseptica FMS-007 is a clinical strain that was isolated from a T-cell non-Hodgkin's lymphoma patient in our laboratory (41). Bioinformatics analysis of the whole genome revealed a fucosidase candidate (core fucosidase I, cFase I) that consisted of 480 amino acids with a predicted signal peptide of 25 amino acids.…”

Section: Bioinformatics Analysis Of a Novel Candidate Fucosidase Genementioning

confidence: 99%

Identification and characterization of a core fucosidase from the bacterium Elizabethkingia meningoseptica

Hou

et al. 2018

Journal of Biological Chemistry

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All reported α-l-fucosidases catalyze the removal of nonreducing terminal l-fucoses from oligosaccharides or their conjugates, while having no capacity to hydrolyze core fucoses in glycoproteins directly. Here, we identified an α-fucosidase from the bacterium with catalytic activity against core α-1,3-fucosylated substrates, and we named it core fucosidase I (cFase I). Using site-specific mutational analysis, we found that three acidic residues (Asp-242, Glu-302, and Glu-315) in the predicted active pocket are critical for cFase I activity, with Asp-242 and Glu-315 acting as a pair of classic nucleophile and acid/base residues and Glu-302 acting in an as yet undefined role. These findings suggest a catalytic mechanism for cFase I that is different from known α-fucosidase catalytic models. In summary, cFase I exhibits glycosidase activity that removes core α-1,3-fucoses from substrates, suggesting cFase I as a new tool for glycobiology, especially for studies of proteins with core fucosylation.

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Section: Bioinformatics Analysis Of a Novel Candidate Fucosidase Genementioning

confidence: 99%

Identification and characterization of a core fucosidase from the bacterium Elizabethkingia meningoseptica

Hou

et al. 2018

Journal of Biological Chemistry

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“…Among the species of genus Elizabethkingia , E. meningosepticum is the most well-known species (9–11). In contrast, less information is available about the epidemiology, virulence factors, antibiotics resistance, and clinical manifestations of E. anophelis .…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequence of Elizabethkingia anophelis Strain EM361-97 Isolated from the Blood of a Cancer Patient

et al. 2016

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“…These findings indicate that E. anophelis can be opportunistically pathogenic in mosquitoes but that the gut provides a barrier against systemic infection (6). Hospital-acquired infections of E. anophelis occur in sick or immunocompromised individuals, sometimes leading to death (2, 7, 8). E. anophelis was the cause of a recent outbreak of nosocomial illness in the Upper Midwest region of the United States (Wisconsin, Illinois and Michigan) (2, 9, 10).…”

Section: Introductionmentioning

confidence: 99%

Elizabethkingia anophelisresponse to iron stress: physiologic, genomic, and transcriptomic analyses

Chen

Johnson

et al. 2019

Preprint

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28Elizabethkingia anophelis bacteria encounter fluxes of iron in the midgut of mosquitoes, 29where they live as symbionts. They also establish bacteremia with severe clinical 30 manifestations in humans, and live in water service lines in hospitals. In this study, we 31 investigated the global gene expression responses of E. anophelis to iron fluxes in the midgut 32 of female Anopheles stephensi mosquitoes fed sucrose or blood, and in iron-poor or iron-rich 33 culture conditions. Of 3,686 transcripts revealed by RNAseq technology, 218 were upregulated 34 while 112 were down-regulated under iron-poor conditions. Most of these differentially 35 expressed genes (DEGs) were enriched in functional groups assigned within "biological 36 process," "cell component" and "molecular function" categories. E. anophelis possessed 4 37 iron/heme acquisition systems. Hemolysin gene expression was significantly repressed when 38 cells were grown under iron-rich or high temperature (37℃) conditions. Furthermore, 39 hemolysin gene expression was down-regulated after a blood meal, indicating that E. anophelis 40 cells responded to excess iron and its associated physiological stress by limiting iron loading. 41By contrast, genes encoding respiratory chain proteins were up-regulated under iron-rich 42 conditions, allowing these iron-containing proteins to chelate intracellular free iron. In vivo 43 studies showed that growth of E. anophelis cells increased 3-fold in blood-fed mosquitoes over 44 those in sucrose-fed ones. Deletion of aerobactin synthesis genes led to impaired cell growth 45 in both iron-rich and iron-poor media. Mutants showed more susceptibility to H 2 O 2 toxicity 46 and less biofilm formation than did wild-type cells. Mosquitoes with E. anophelis 47 experimentally colonized in their guts produced more eggs than did those treated with 48 erythromycin or left unmanipulated, as controls. Results reveal that E. anophelis bacteria 49 respond to varying iron concentration in the mosquito gut, harvest iron while fending off iron-50 associated stress, contribute to lysis of red blood cells, and positively influence mosquito host 51 fecundity. 52

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Complete Genome Sequence of Elizabethkingia meningoseptica, Isolated from a T-Cell Non-Hodgkin's Lymphoma Patient

Cited by 17 publications

References 7 publications

Identification and characterization of a core fucosidase from the bacterium Elizabethkingia meningoseptica

Identification and characterization of a core fucosidase from the bacterium Elizabethkingia meningoseptica

Draft Genome Sequence of Elizabethkingia anophelis Strain EM361-97 Isolated from the Blood of a Cancer Patient

Elizabethkingia anophelisresponse to iron stress: physiologic, genomic, and transcriptomic analyses

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