Complete Genome Sequence of Ebrios, a Novel T7virus Isolated from the Ebrie Lagoon in Abidjan, Côte d’Ivoire

Ngazoa-Kakou, Solange; Philippe, Cécile; Tremblay, Denise M.; Loignon, Stéphanie; Koudou, Aristide; Abole, Ahmed; Coulibaly, David N’golo; Kouassi, Stéphane Kan; Kouamé-Sina, Sylvie Mireille; Aoussi, Serge; Dosso, Mireille; Moineau, Sylvain

doi:10.1128/genomea.00280-18

Cited by 5 publications

(5 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Coliphages in general are one of the most sequenced phage types with ~450 complete phage genomes within Genbank, isolated from a variety of sources including animal faeces (Niu et al, ; Smith et al, ; Sazinas et al, ; Golomidova et al, ), human faeces (Dalmasso et al, ), urine (Malki et al, ), river water (Alijošius et al, ), agricultural surface waters (Liao et al, ), lagoons (Ngazoa‐Kakou et al, ), sewage (Trotereau et al, ) and animal slurries (Sazinas et al, ). However, much less is known about the genetic diversity of coliphages in seawater.…”

Section: Introductionmentioning

confidence: 99%

Riding the wave of genomics to investigate aquatic coliphage diversity and activity

Michniewski

Redgwell

Grigonyte

et al. 2019

Environmental Microbiology

View full text Add to dashboard Cite

Summary Bacteriophages infecting Escherichia coli (coliphages) have been used as a proxy for faecal matter and water quality from a variety of environments. However, the diversity of coliphages that is present in seawater remains largely unknown, with previous studies largely focusing on morphological diversity. Here, we isolated and characterized coliphages from three coastal locations in the United Kingdom and Poland. Comparative genomics and phylogenetic analysis of phage isolates facilitated the identification of putative new species within the genera Rb69virus and T5virus and a putative new genus within the subfamily Tunavirinae. Furthermore, genomic and proteomic analysis combined with host range analysis allowed the identification of a putative tail fibre that is likely responsible for the observed differences in host range of phages vB_Eco_mar003J3 and vB_Eco_mar004NP2.

show abstract

Section: Introductionmentioning

confidence: 99%

Riding the wave of genomics to investigate aquatic coliphage diversity and activity

Michniewski

Redgwell

Grigonyte

et al. 2019

Environmental Microbiology

View full text Add to dashboard Cite

show abstract

“…Several studies have reported isolating bacteriophages from wastewater (18,19). They are usually detected by lytic activity on the host bacteria.…”

Section: Discussionmentioning

confidence: 99%

Isolation and biocontrol of bacteriophages from wastewater in the city of Lomé, Togo: potential application as a novel source for antimicrobial therapy

Ouedraogo,

Hoekou,

Gbekley

et al. 2024

Af J Clin Exp Micro

View full text Add to dashboard Cite

Background: Bacteriophages offer one of the most promising solutions to the challenges of antimicrobial resistance in bacteria. The aim of this study is to investigate bacteriophages as a source of new antimicrobial therapy. Methodology: Waste water samples were randomly collected from 8 different locations in the city of Lomé for bacteriophage isolation. The phages were isolated using multi-resistant clinical isolates (Escherichia coli 1642 and Staphylococcus aureus 0868) as hosts by means of a spot test. The host range of the phages was determined also by a spot test using 8 other clinical bacterial isolates including two reference strains (E. coli ATCC 25922 and S. aureus ATCC 29213). The virulence of the phages and their effects on bacterial growth were assessed by in vitro experiments using E. coli 1642 BBec phage suspension. Results: Isolation of phages by the spot test was positive only with the host E. coli 1642. A reduced host range was observed with the other bacteria. The BBec phage suspension showed a titer of 1.6 x 107 PFU/ml. Virulence studies revealed a latency time of less than 10 minutes, a degree of absorption of 87% and a burst size of 63 PFU/cell. The effect of BBec phage suspension on E. coli 1642 showed an almost total reduction in the population of E. coli 1642 after 4 hours. Conclusion: This study provided scientific data showing the antibacterial effect of a phage suspension (BBec) on a multi-resistant clinical isolate of E. coli 1642. This phage could therefore be explored as a candidate for the development of new antibacterial therapies. Contexte : Face aux problèmes de multirésistance des bactéries aux agents antimicrobiens, les bactériophages représentent l’une des solutions les plus prometteuses. L’objectif de ce travail est d’étudier les bactériophages en tant que source de nouvelles thérapies antimicrobiennes . Méthodologie : Des échantillons d'eaux usées ont été collectés de manière aléatoire dans 8 endroits différents de la ville de Lomé pour l'isolement des bactériophages. Les phages ont été isolés en utilisant comme hôtes, des isolats cliniques ( Escherichia coli 1642 et Staphylococcus aureus 0868 ) multirésistants par le biais d’un test ponctuel. La gamme d’hôte des phages a également été déterminée par un test ponctuel utilisant 8 autres isolats dont deux souches de référence ( E. coli ATCC 25922 et S. aureus 29213)29213). L’évaluation de la virulence des phages et leurs effets sur la croissance des bactéries ont été réalisés à travers des expérimentations in vitro avec une suspension de phages d’ E. coli désignée BB ec . Résultats : L’isolement des phages par le test ponctuel s’est révélé positif seulement avec l’hôte E. coli 1642. Une gamme d’hôte réduite a été observée avec les autres bactéries. La suspension de phage BBec a présenté un titre de 1,6 x 107 UFP/ml. L’étude de sa virulence a révélé un temps de latence inférieur à 10 minutes, un degré d’absorption de 87% et une taille de rafale de 63 UFP/Cellule. L’effet de la suspension phagique BBec sur l’isolat E. coli 1642 a montré une réduction quasi totale de la population de l’isolat E. coli 1642 au bout de 4 h. Conclusion : Cette étude a permis de fournir des données scientifiques qui montrent l’effet antibactérien d’une suspension de phage (BBec) sur un isolat clinique multirésistant E. coli 1642. Ce ph age pourrait donc être exploré comme candidat au développement de nouvelles thérapies antibactériennes.

show abstract

“…To establish a phage biobank for biocontrol purposes in Côte d’Ivoire, new phages are being isolated and characterized ( 1 – 3 ). Escherichia phages Carena and JoYop were previously isolated from the samples collected in Ebrié Lagoon (latitude 5.356322; longitude −4.091498) and in wastewater of Yopougon (latitude 5.327334; longitude −4.028653), respectively ( 1 ).…”

Section: Announcementmentioning

confidence: 99%

Complete genome of Escherichia phages Carena and JoYop

Addablah,

Ngazoa-Kakou,

Adioumani

et al. 2024

Microbiol Resour Announc

Self Cite

View full text Add to dashboard Cite

Escherichia phages Carena and JoYop were isolated from water samples in Abidjan (Cote d’Ivoire). Their genomes comprise 39,283 and 169,193 bp, encoding 44 and 246 predicted genes, respectively. Carena shares 93.4% nucleotide identity with Escherichia podophage CarlSpitteler ( Berlinvirus ), and JoYop shows 95.6% identity with Escherichia myophage ADUt ( Tequatrovirus ).

show abstract

Complete Genome Sequence of Ebrios, a Novel T7virus Isolated from the Ebrie Lagoon in Abidjan, Côte d’Ivoire

Cited by 5 publications

References 11 publications

Riding the wave of genomics to investigate aquatic coliphage diversity and activity

Riding the wave of genomics to investigate aquatic coliphage diversity and activity

Isolation and biocontrol of bacteriophages from wastewater in the city of Lomé, Togo: potential application as a novel source for antimicrobial therapy

Complete genome of Escherichia phages Carena and JoYop

Contact Info

Product

Resources

About