Complete Genome Sequence of Corynebacterium pseudotuberculosis Strain E19, Isolated from a Horse in Chile

Cavalcante, Ana Lidia Queiroz; Dias, Larissa Maranhão; Alves, Jorianne Thyeska Castro; Veras, Adonney Allan de Oliveira; Guimarães, Luís Carlos; Rocha, Flávia Souza; Gala-García, Alfonso; Retamal, Patricio; Ramos, Rommel Thiago Jucá; Azevedo, Vasco; Silva, Artur M. S.; Carneiro, Adriana Ribeiro

doi:10.1128/genomea.01385-15

Cited by 3 publications

(3 citation statements)

References 12 publications

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“…In the USA, high incidence rates of the disease have been described in Texas [ 9 ], Colorado [ 11 ] California [ 12 ] and several other states [ 8 ]. This problem is not restricted to North America, however, and several other strains have been isolated from animals around the world, such as in Chile [ 13 ] and Egypt [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Assessing the Genotypic Differences between Strains of Corynebacterium pseudotuberculosis biovar equi through Comparative Genomics

et al. 2017

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Seven genomes of Corynebacterium pseudotuberculosis biovar equi were sequenced on the Ion Torrent PGM platform, generating high-quality scaffolds over 2.35 Mbp. This bacterium is the causative agent of disease known as “pigeon fever” which commonly affects horses worldwide. The pangenome of biovar equi was calculated and two phylogenomic approaches were used to identify clustering patterns within Corynebacterium genus. Furthermore, other comparative analyses were performed including the prediction of genomic islands and prophages, and SNP-based phylogeny. In the phylogenomic tree, C. pseudotuberculosis was divided into two distinct clades, one formed by nitrate non-reducing species (biovar ovis) and another formed by nitrate-reducing species (biovar equi). In the latter group, the strains isolated from California were more related to each other, while the strains CIP 52.97 and 1/06-A formed the outermost clade of the biovar equi. A total of 1,355 core genes were identified, corresponding to 42.5% of the pangenome. This pangenome has one of the smallest core genomes described in the literature, suggesting a high genetic variability of biovar equi of C. pseudotuberculosis. The analysis of the similarity between the resistance islands identified a higher proximity between the strains that caused more severe infectious conditions (infection in the internal organs). Pathogenicity islands were largely conserved between strains. Several genes that modulate the pathogenicity of C. pseudotuberculosis were described including peptidases, recombination enzymes, micoside synthesis enzymes, bacteriocins with antimicrobial activity and several others. Finally, no genotypic differences were observed between the strains that caused the three different types of infection (external abscess formation, infection with abscess formation in the internal organs, and ulcerative lymphangitis). Instead, it was noted that there is a higher phenetic correlation between strains isolated at California compared to the other strains. Additionally, high variability of resistance islands suggests gene acquisition through several events of horizontal gene transfer.

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Section: Introductionmentioning

confidence: 99%

Assessing the Genotypic Differences between Strains of Corynebacterium pseudotuberculosis biovar equi through Comparative Genomics

et al. 2017

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“…Previous reports have solely documented the infection of goats with C. pseudotuberculosis biovar ovis strains in Sichuan Province, China ( Zhang et al, 2015 ). While the alpacas in this study were imported from Chile and the Netherlands, it is worth noting that in Chile, cases of C. pseudotuberculosis biovar equi strains infection in horses have been reported ( Cavalcante et al, 2015 ). Therefore, the pathogenic bacteria isolated in this study may have originated from the country from where the alpacas were introduced.…”

Section: Discussionmentioning

confidence: 99%

Isolation, characterization, and pathogenicity assessment of Corynebacterium pseudotuberculosis biovar equi strains from alpacas (Vicugna pacos) in China

et al. 2023

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Corynebacterium pseudotuberculosis is a zoonotic pathogen that causes lymphadenitis in humans, livestock, and wildlife. In this study, C. pseudotuberculosis biovar equi strains were isolated from three alpacas. Antibiotic susceptibility tests and pathogenicity tests were also conducted. Moreover, one strain was sequenced using DNBSEQ and Oxford Nanopore technology. The three strains exhibited resistance to aztreonam, fosfomycin, and nitrofurantoin. The median lethal doses (LD50) of strains G1, S2 and BA3 in experimentally infected mice was 1.66 × 105 CFU, 3.78 × 105 CFU and 3.78 × 105 CFU, respectively. The sequencing of strain G1 resulted in the assembly of a chromosomal scaffold comprising 2,379,166 bp with a G + C content of 52.06%. Genome analysis of strain G1 revealed the presence of 48 virulence genes and 5 antibiotic resistance genes (ARGs). Comparative genomic analysis demonstrates a high degree of genetic similarity among C. pseudotuberculosis strains, in contrast to other Corynebacterium species, with a clear delineation between strains belonging to the two biovars (ovis and equi). The data of the present study contribute to a better understanding of the properties of C. pseudotuberculosis biovar equi strains and the potential risk they pose to alpacas and other livestock, as well as the necessity of ongoing surveillance and monitoring of infectious diseases in animals.

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“…Cumulatively, these studies confirm that this biovar is more widespread than originally suspected and can be found in many regions of the world (Cavalcante et al . ).…”

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confidence: 97%

Corynebacterium pseudotuberculosis infection in horses: Increasing frequency and spread to new regions of North America

Spier

Azevedo

2016

Equine Veterinary Education

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Complete Genome Sequence of Corynebacterium pseudotuberculosis Strain E19, Isolated from a Horse in Chile

Cited by 3 publications

References 12 publications

Assessing the Genotypic Differences between Strains of Corynebacterium pseudotuberculosis biovar equi through Comparative Genomics

Assessing the Genotypic Differences between Strains of Corynebacterium pseudotuberculosis biovar equi through Comparative Genomics

Isolation, characterization, and pathogenicity assessment of Corynebacterium pseudotuberculosis biovar equi strains from alpacas (Vicugna pacos) in China

Corynebacterium pseudotuberculosis infection in horses: Increasing frequency and spread to new regions of North America

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