Complete Genome Sequence of Chikungunya Virus Isolated in the Philippines

Kawashima, Kent; Suarez, Lady Anne C.; Labayo, Hannah Karen Mina; Liles, Veni R; Salvoza, Noel; Klinzing, David C.; Daroy, Maria Luisa G; Matias, Ronald R.; Natividad, Filipinas F.

doi:10.1128/genomea.00336-14

Cited by 10 publications

(6 citation statements)

References 9 publications

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“…aegypti, increasing the virus infectivity, dissemination and transmission, compared to the E1 A226V virus [61]. In the same way, three non-synonymous mutations (E2 S248F and NSP3 A437T and L451F) were present in all of our sequences, as well as in the sequences from the Caribbean outbreak [62], pointing at this geographic region as the origin of the virus that caused the Mexican outbreak, as previously inferred [26].…”

Section: Discussionsupporting

confidence: 85%

Genetic Diversity and Spatiotemporal Dynamics of Chikungunya Infections in Mexico during the Outbreak of 2014–2016

Rodríguez-Aguilar

Martínez-Barnetche

González-Bonilla

et al. 2021

Viruses

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Chikungunya virus (CHIKV) is an alphavirus transmitted by Aedes mosquitoes, which causes Chikungunya fever. Three CHIKV genotypes have been identified: West African, East-Central-South African and Asian. In 2014, CHIKV was detected for the first time in Mexico, accumulating 13,569 confirmed cases in the following three years. Studies on the molecular diversification of CHIKV in Mexico focused on limited geographic regions or investigated only one structural gene of the virus. To describe the dynamics of this outbreak, we analyzed 309 serum samples from CHIKV acute clinical cases from 15 Mexican states. Partial NSP3, E1, and E2 genes were sequenced, mutations were identified, and their genetic variability was estimated. The evolutionary relationship with CHIKV sequences sampled globally were analyzed. Our sequences grouped with the Asian genotype within the Caribbean lineage, suggesting that the Asian was the only circulating genotype during the outbreak. Three non-synonymous mutations (E2 S248F and NSP3 A437T and L451F) were present in our sequences, which were also identified in sequences of the Caribbean lineage and in one Philippine sequence. Based on the phylogeographic analysis, the viral spread was reconstructed, suggesting that after the introduction through the Mexican southern border (Chiapas), CHIKV dispersed to neighboring states before reaching the center and north of the country through the Pacific Ocean states and Quintana Roo. This is the first viral phylogeographic reconstruction in Mexico characterizing the CHIKV outbreak across the country.

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Section: Discussionsupporting

confidence: 85%

Genetic Diversity and Spatiotemporal Dynamics of Chikungunya Infections in Mexico during the Outbreak of 2014–2016

Rodríguez-Aguilar

Martínez-Barnetche

González-Bonilla

et al. 2021

Viruses

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“…CHIKV is an enveloped, positive strand RNA virus with a genome usually a little less than 12000 nucleotides. Its genome consists of five structural proteins; E1, E2, E3, C (Capsid protein), 6K, and four non-structural proteins; non-structural proteins 1 (nsP1), 2 (nsP2), 3 (nsP3) and 4 (nsP4) [15–16]. Evolutionary studies on the virus provide evidence that the West African lineage of CHIKV originated in Africa and subsequently moved into Asia where it evolved into a distinct Asian lineage.…”

Section: Introductionmentioning

confidence: 99%

A scoping review of published literature on chikungunya virus

et al. 2018

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Chikungunya virus (CHIKV) has caused several major epidemics globally over the last two decades and is quickly expanding into new areas. Although this mosquito-borne disease is self-limiting and is not associated with high mortality, it can lead to severe, chronic and disabling arthritis, thereby posing a heavy burden to healthcare systems. The two main vectors for CHIKV are Aedes aegypti and Aedes albopictus (Asian tiger mosquito); however, many other mosquito species have been described as competent CHIKV vectors in scientific literature. With climate change, globalization and unfettered urban planning affecting many areas, CHIKV poses a significant public health risk to many countries. A scoping review was conducted to collate and categorize all pertinent information gleaned from published scientific literature on a priori defined aspects of CHIKV and its competent vectors. After developing a sensitive and specific search algorithm for the research question, seven databases were searched and data was extracted from 1920 relevant articles. Results show that CHIKV research is reported predominantly in areas after major epidemics have occurred. There has been an upsurge in CHIKV publications since 2011, especially after first reports of CHIKV emergence in the Americas. A list of hosts and vectors that could potentially be involved in the sylvatic and urban transmission cycles of CHIKV has been compiled in this scoping review. In addition, a repository of CHIKV mutations associated with evolutionary fitness and adaptation has been created by compiling and characterizing these genetic variants as reported in scientific literature.

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“…The subsequent outbreaks in Italy in 2007 [ 21 ], and in Singapore in 2008 and 2013 further emphasized the ability of CHIKV to thrive within urbanized areas and not just primarily in developing countries [ 22 , 23 ]. Other countries in Southeast Asia were also hit with CHIKV during this period, with outbreaks occurring in Malaysia [ 24 ], Thailand [ 25 ] and rural villages in the Philippines [ 26 ]. The recent CHIKV outbreaks in the Caribbean Islands [ 18 ] have raised the possibility of a worldwide transmission.…”

Section: Introductionmentioning

confidence: 99%

Limitations of Current in Vivo Mouse Models for the Study of Chikungunya Virus Pathogenesis

Chan

Lum

2015

Medical Sciences

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Chikungunya virus (CHIKV) is an arthropod-borne alphavirus that causes febrile chikungunya fever (CHIKF) in humans. This disease is debilitating and characterized by acute fever onset and chronic incapacitating polyarthralgia. CHIKF pathogenesis remains poorly defined with no approved vaccines and therapies. Recent outbreaks in the Caribbean islands have elevated concerns over the possibility of a global pandemic. Tremendous efforts have been made to develop relevant mouse models to enable the study of infection and immunity against this viral disease. Among them, the more common C57BL/6 mouse model demonstrated the ability to recapitulate the symptoms shown in infected humans, including self-limiting arthritis, myositis, and tenosynovitis. This has facilitated the unraveling of some key factors involved in disease pathogenesis of CHIKF. However, the stark differences in immune response between humans and mouse models necessitate the development of an animal model with an immune system that is more genetically similar to the human system for a better representation. In this paper, we aim to uncover the limitations of the C57BL/6 model and discuss alternative mouse models for CHIKV research.

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Complete Genome Sequence of Chikungunya Virus Isolated in the Philippines

Cited by 10 publications

References 9 publications

Genetic Diversity and Spatiotemporal Dynamics of Chikungunya Infections in Mexico during the Outbreak of 2014–2016

Genetic Diversity and Spatiotemporal Dynamics of Chikungunya Infections in Mexico during the Outbreak of 2014–2016

A scoping review of published literature on chikungunya virus

Limitations of Current in Vivo Mouse Models for the Study of Chikungunya Virus Pathogenesis

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