Complete genome sequence of carotenoid-producing Microbacterium sp. strain PAMC28756 isolated from an Antarctic lichen

Han, So-Ra; Kim, Ki-Hwa; Ahn, Do Hwan; Park, Hyun; Oh, Tae‐Jin

doi:10.1016/j.jbiotec.2016.03.034

Cited by 22 publications

(10 citation statements)

References 8 publications

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“…Microbacterium sp. is an actinomycete that is widespread in nature and can be found in diverse and extreme environments such as Antarctica, desertic soils, and in oil refinery plants 21,22 . A number of species in this genus are known species with several biotechnological applications synthesizing bioproducts including amylases and other enzymes capable of degrading multiple polycyclic aromatic hydrocarbons and biosurfactants 23 .…”

Section: Resultsmentioning

confidence: 99%

Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01

Reis-Mansur

Cardoso-Rurr

Silva

et al. 2019

Sci Rep

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The Microbacterium sp. LEMMJ01 isolated from Antarctic soil does not belong to any of the nearest species identified in the RDP database. Under UV radiation (A, B and C wavebands) the survival fractions of Microbacterium sp. cells were much higher compared with wild-type E . coli K12A15. Especially remarkable for an Antarctic bacterium, an expressive resistance against high UV-B doses was observed. The increased survival of DNA repair-proficient E . coli grown overnight added of 0.1 mg/ml or 1 mg/ml of the whole pigment extract produced by Microbacterium sp. revealed that part of the resistance of Microbacterium sp. against UV-B radiation seems to be connected with photoprotection by its pigments. Scanning electron microscopy revealed that UV-A and UV-B ensued membrane alterations only in E . coli . The APCI-MS fingerprints revealed the diagnostic ions for neurosporene (m/z 580, 566, 522, 538, and 524) synergism for the first time in this bacterium by HPLC-MS/MS analysis. Carotenoids also were devoid of phototoxicity and cytotoxicity effects in mouse cells and in human keratinocytes and fibroblasts.

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Section: Resultsmentioning

confidence: 99%

Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01

Reis-Mansur

Cardoso-Rurr

Silva

et al. 2019

Sci Rep

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“…Not only Arthrobacter but also the genus Microbacterium, the second most abundant one with 14 representatives, is also known for its ability to synthetize carotenoids. Han et al (2016) and Reis-Mansur et al (2019) reported the UV resistance of two carotenoidproducing Microbacterium spp., both isolated from Antarctica. Furthermore, the genus Methylobacterium, represented by six isolates in the collection, has been described to synthetize pink carotenoids, which presumably act as antioxidants against reactive oxygen species (ROS) generated by UVA and UVB stresses (Sundin et al, 2002;Gao and Garcia-Pichel, 2011).…”

Section: Discussion the Tabernas Desert Soils Harbor A Wide Bacterialmentioning

confidence: 99%

High Culturable Bacterial Diversity From a European Desert: The Tabernas Desert

et al. 2021

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One of the most diverse ecological niches for microbial bioprospecting is soil, including that of drylands. Drylands are one of the most abundant biomes on Earth, but extreme cases, such as deserts, are considered very rare in Europe. The so-called Tabernas Desert is one of the few examples of a desert area in continental Europe, and although some microbial studies have been performed on this region, a comprehensive strategy to maximize the isolation of environmental bacteria has not been conducted to date. We report here a culturomics approach to study the bacterial diversity of this dryland by using a simple strategy consisting of combining different media, using serial dilutions of the nutrients, and using extended incubation times. With this strategy, we were able to set a large (254 strains) collection of bacteria, the majority of which (93%) were identified through 16S ribosomal RNA (rRNA) gene amplification and sequencing. A significant fraction of the collection consisted of Actinobacteria and Proteobacteria, as well as Firmicutes strains. Among the 254 isolates, 37 different genera were represented, and a high number of possible new taxa were identified (31%), of which, three new Kineococcus species. Moreover, 5 out of the 13 genera represented by one isolate were also possible new species. Specifically, the sequences of 80 isolates held a percentage of identity below the 98.7% threshold considered for potentially new species. These strains belonged to 20 genera. Our results reveal a clear link between medium dilution and isolation of new species, highlight the unexploited bacterial biodiversity of the Tabernas Desert, and evidence the potential of simple strategies to yield surprisingly large numbers of diverse, previously unreported, bacterial strains and species.

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“…Microbacterium strains have been found in various extremophilic locations, including Antarctic habitats [26], the Atacama Desert [27], sediment of the Indian Ocean [28], uranium-rich soil [29] and salty soil [30]. To be active in these extremophilic locations, enzymes from local Microbacterium strains may have to evolve.…”

Section: Discussionmentioning

confidence: 99%

Distinctive molecular and biochemical characteristics of a glycoside hydrolase family 20 β-N-acetylglucosaminidase and salt tolerance

et al. 2017

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BackgroundEnzymatic degradation of chitin has attracted substantial attention because chitin is an abundant renewable natural resource, second only to lignocellulose, and because of the promising applications of N-acetylglucosamine in the bioethanol, food and pharmaceutical industries. However, the low activity and poor tolerance to salts and N-acetylglucosamine of most reported β-N-acetylglucosaminidases limit their applications. Mining for novel enzymes from new microorganisms is one way to address this problem.ResultsA glycoside hydrolase family 20 (GH 20) β-N-acetylglucosaminidase (GlcNAcase) was identified from Microbacterium sp. HJ5 harboured in the saline soil of an abandoned salt mine and was expressed in Escherichia coli. The purified recombinant enzyme showed specific activities of 1773.1 ± 1.1 and 481.4 ± 2.3 μmol min−1 mg−1 towards p-nitrophenyl β-N-acetylglucosaminide and N,N'-diacetyl chitobiose, respectively, a V max of 3097 ± 124 μmol min−1 mg−1 towards p-nitrophenyl β-N-acetylglucosaminide and a K i of 14.59 mM for N-acetylglucosamine inhibition. Most metal ions and chemical reagents at final concentrations of 1.0 and 10.0 mM or 0.5 and 1.0% (v/v) had little or no effect (retaining 84.5 − 131.5% activity) on the enzyme activity. The enzyme can retain more than 53.6% activity and good stability in 3.0–20.0% (w/v) NaCl. Compared with most GlcNAcases, the activity of the enzyme is considerably higher and the tolerance to salts and N-acetylglucosamine is much better. Furthermore, the enzyme had higher proportions of aspartic acid, glutamic acid, alanine, glycine, random coils and negatively charged surfaces but lower proportions of cysteine, lysine, α-helices and positively charged surfaces than its homologs. These molecular characteristics were hypothesised as potential factors in the adaptation for salt tolerance and high activity of the GH 20 GlcNAcase.ConclusionsBiochemical characterization revealed that the GlcNAcase had novel salt–GlcNAc tolerance and high activity. These characteristics suggest that the enzyme has versatile potential in biotechnological applications, such as bioconversion of chitin waste and the processing of marine materials and saline foods. Molecular characterization provided an understanding of the molecular–function relationships for the salt tolerance and high activity of the GH 20 GlcNAcase.Electronic supplementary materialThe online version of this article (doi:10.1186/s12896-017-0358-1) contains supplementary material, which is available to authorized users.

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Complete genome sequence of carotenoid-producing Microbacterium sp. strain PAMC28756 isolated from an Antarctic lichen

Cited by 22 publications

References 8 publications

Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01

Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01

High Culturable Bacterial Diversity From a European Desert: The Tabernas Desert

Distinctive molecular and biochemical characteristics of a glycoside hydrolase family 20 β-N-acetylglucosaminidase and salt tolerance

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