Complete Genome Sequence of Bifidobacterium longum subsp. longum KACC 91563

Abstract

Bifidobacterium longum strains predominate in the colonic microbiota of breast-fed infants. Here we report the complete genome sequence of B. longum subsp. longum KACC 91563, isolated from feces of neonates. A single circular chromosome of 2,385,301 bp contains 1,980 protein-coding genes, 56 tRNA genes, and 3 rRNA operons.Members of the genus Bifidobacterium are prevalent in the human colon and represent up to 90% of all bacteria in fecal samples of breast-fed infants and 3 to 5% of adult fecal microbiota (7,8). Currently the genus harbors 32 species (2), and among these, B. longum strains often predominate in the colonic microbiota of breast-fed infants (4, 5). Many studies suggest that Bifidobacterium longum provides potential health benefits to human over a wide range of health conditions, for example, cholesterol reduction (11) and cancer prevention (9). Here we report the complete genome sequence, including its two plasmids, of B. longum subsp. longum KACC 91563, isolated from feces of healthy neonates.The entire genome and two plasmids of B. longum KACC 91563 were sequenced using a Roche 454 Life Sciences GS FLX sequencer with paired-end and shotgun libraries (222ϫ coverage) at the National

Extracellular vesicle–derived protein from Bifidobacterium longum alleviates food allergy through mast cell suppression

“…19,26,27 According to TEM analysis, B longum KACC 91563 and E faecalis KACC 91532 are rod-shaped and roundshaped bacteria, respectively (see Fig E1, A, in this article's Online Repository at www.jacionline.org). Because it was uncertain whether these 2 kinds of microbiota would reach the intestines of mice intact, we orally administered E faecalis KACC 91532 and B longum KACC 91563 for 2 weeks and observed bacteria in fecal samples using FISH.…”

Quality Characteristics of Functional Fermented Sausages Added with Encapsulated Probiotic Bifidobacterium longum KACC 91563

“…Bifidobacteria are Gram-positive bacteria which are commonly present in healthy human’s intestinal tract; especially they occupy approximately 90% of total bacteria in feces of breast-fed infants and about 3% to 5% in adult fecal microbiota (Ham et al, 2011). Through the application of genomic sequencing technique, it has been shown that Bifidobacterium longum is absent from virulence and pathogenicity factors and it is considered as the safest bacteria without health hazards (Meile et al, 2008).…”

Metabolism of Ginsenoside Rb1 by Human Intestinal Microflora and Cloning of Its Metabolizing β-<small>D</small>-Glucosidase from <i>Bifidobacterium longum</i> H-1

“…These products are the respective metabolites biotransformed by intestinal microflora, when loganin, arctiin and ginsenoside Rb1 were orally administered. 1,26) The metabolites arctigenin and ginsenoside Rd exhibit more potent biological effects in vitro than their parental compounds. 37,38) However, the biological activities of loganetin, a metabolite of loganin, have not been studied.…”

“…longum KACC91563 (GenBank protein accession number CP002794). 26) Expression and Purification of the rBglX In order to investigate the enzymatic property of the rBglX in E. coli, the rBglX was expressed by inserting the cloned gene along with the codon of its signal peptide into the KpnI and HindIII sites of pET-39b(+) vector, which has N-terminal and C-terminal 6×His tag. The pET 39b(+) vector has DsbA, which is the periplasmic enzyme that catalyzes the formation and isomerization of disulfide bond and is used for the purpose of enhancing solubility.…”