Complete Genome Sequence of Bacillus cereus NC7401, Which Produces High Levels of the Emetic Toxin Cereulide

Takeno, Akira; Okamoto, Akira; Tori, Keizo; Oshima, Koichiro; Hirakawa, Hideki; Toh, Y.; Agata, Norio; Yamada, Keiko; Ogasawara, Nagahisa; Hayashi, Tetsuya; Shimizu, Tohru; Kuhara, Satoru; Hattori, Masahira; Ohta, Michio

doi:10.1128/jb.01015-12

Cited by 24 publications

(10 citation statements)

References 19 publications

(13 reference statements)

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“…This isolate tested positive for emetic toxin (cereulide) formation and nonhemolytic enterotoxin (NHE) and negative for HBL hemolytic enterotoxin and cytotoxin K, and it belonged to MLST ST26 ( Table 5 ) ( 73 ); these findings were confirmed using BTyper. Other notable strains in this cluster included (i) emetic strain B. cereus H3081.97, a B. cereus strain of sequence type 144 (ST144) which is closely related to strain AH187, and (ii) emetic strain B. cereus NC7401 ( 74 ).…”

Section: Resultsmentioning

confidence: 99%

Rapid, High-Throughput Identification of Anthrax-Causing and Emetic Bacillus cereus Group Genome Assemblies via BTyper, a Computational Tool for Virulence-Based Classification of Bacillus cereus Group Isolates by Using Nucleotide Sequencing Data

Carroll

Kovač

Miller

et al. 2017

Appl Environ Microbiol

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The Bacillus cereus group comprises nine species, several of which are pathogenic. Differentiating between isolates that may cause disease and those that do not is a matter of public health and economic importance, but it can be particularly challenging due to the high genomic similarity within the group. To this end, we have developed BTyper, a computational tool that employs a combination of (i) virulence gene-based typing, (ii) multilocus sequence typing (MLST), (iii) panC clade typing, and (iv) rpoB allelic typing to rapidly classify B. cereus group isolates using nucleotide sequencing data. BTyper was applied to a set of 662 B. cereus group genome assemblies to (i) identify anthrax-associated genes in non-B. anthracis members of the B. cereus group, and (ii) identify assemblies from B. cereus group strains with emetic potential. With BTyper, the anthrax toxin genes cya, lef, and pagA were detected in 8 genomes classified by the NCBI as B. cereus that clustered into two distinct groups using k-medoids clustering, while either the B. anthracis poly-␥-D-glutamate capsule biosynthesis genes capABCDE or the hyaluronic acid capsule hasA gene was detected in an additional 16 assemblies classified as either B. cereus or Bacillus thuringiensis isolated from clinical, environmental, and food sources. The emetic toxin genes cesABCD were detected in 24 assemblies belonging to panC clades III and VI that had been isolated from food, clinical, and environmental settings. The command line version of BTyper is available at https://github.com/lmc297/BTyper. In addition, BMiner, a companion application for analyzing multiple BTyper output files in aggregate, can be found at https://github.com/lmc297/BMiner. IMPORTANCE Bacillus cereus is a foodborne pathogen that is estimated to cause tens of thousands of illnesses each year in the United States alone. Even with molecular methods, it can be difficult to distinguish nonpathogenic B. cereus group isolates from their pathogenic counterparts, including the human pathogen Bacillus anthracis, which is responsible for anthrax, as well as the insect pathogen B. thuringiensis. By using the variety of typing schemes employed by BTyper, users can rapidly classify, characterize, and assess the virulence potential of any isolate using its nucleotide sequencing data.KEYWORDS Bacillus cereus group, taxonomy, virulence genes, whole-genome sequencing

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Section: Resultsmentioning

confidence: 99%

Rapid, High-Throughput Identification of Anthrax-Causing and Emetic Bacillus cereus Group Genome Assemblies via BTyper, a Computational Tool for Virulence-Based Classification of Bacillus cereus Group Isolates by Using Nucleotide Sequencing Data

Carroll

Kovač

Miller

et al. 2017

Appl Environ Microbiol

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“…2.0 software (Pacific Biosciences). Gene prediction was performed using Glimmer 3 [6], and annotations were performed by a homology search against the SEED database, Universal Protein Resource (UniProt) database, and eggNOG database [7,13,24,26]. In silico DNA-DNA hybridization values of the strain FORC_021 with the complete genome sequences of B. cereus from a public database were calculated with the Genome-to-Genome Distance Calculator (GGDC; http://ggdc.dsmz.de).…”

Section: Genome Sequencing Annotation and Comparisonmentioning

confidence: 99%

“…Keywords: Bacillus cereus, food-borne pathogen, virulence factor, enterotoxin, Listeria pathogenicity island 1 (LIPI-1) S S comparison of entire genomes [12,17], and therefore, obtaining genome sequences of isolated strains is crucial to identify their characteristics. Several studies have analyzed the B. cereus genomes to understand its toxicity [3,16,20,24].…”

Section: Introductionmentioning

confidence: 99%

Genome Sequence of Bacillus cereus FORC_021, a Food-Borne Pathogen Isolated from a Knife at a Sashimi Restaurant

Chung

Lee

Ryu

et al. 2016

Journal of Microbiology and Biotechnology

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causes food-borne illness through contaminated foods; therefore, its pathogenicity and genome sequences have been analyzed in several studies. We sequenced and analyzed strain FORC_021 isolated from a sashimi restaurant. The genome sequence consists of 5,373,294 bp with 35.36% GC contents, 5,350 predicted CDSs, 42 rRNA genes, and 107 tRNA genes. Based on in silico DNA-DNA hybridization values, ATCC 14579 was closest to FORC_021 among the complete genome-sequenced strains. Three major enterotoxins were detected in FORC_021. Comparative genomic analysis of FORC_021 with ATCC 14579 revealed that FORC_021 harbored an additional genomic region encoding virulence factors, such as putative ADP-ribosylating toxin, spore germination protein, internalin, and sortase. Furthermore, in vitro cytotoxicity testing showed that FORC_021 exhibited a high level of cytotoxicity toward INT-407 human epithelial cells. This genomic information of FORC_021 will help us to understand its pathogenesis and assist in managing food contamination.

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“…Bacillus cereus NC7401, sequence accession AP007209 (chromosome), AP007210 (plasmid pNCcld), AP007211 (plasmid pNC1, 48 kb), AP007212 (plasmid pNC2, 5 kb), AP007213 (plasmid pNC3, 4 kb), and AP007214 (plasmid pNC4, 3 kb) [82]…”

Section: Bacteriamentioning

confidence: 99%

Genome sequences published outside of Standards in Genomic Sciences, July - October 2012

Nelson¹

2012

Stand. Genomic Sci.

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The purpose of this table is to provide the community with a citable record of publications of ongoing genome sequencing projects that have led to a publication in the scientific literature. While our goal is to make the list complete, there is no guarantee that we may have omitted one or more publications appearing in this time frame. Readers and authors who wish to have publications added to subsequent versions of this list are invited to provide the bibliographic data for such references to the SIGS editorial office.

show abstract

Complete Genome Sequence of Bacillus cereus NC7401, Which Produces High Levels of the Emetic Toxin Cereulide

Cited by 24 publications

References 19 publications

Rapid, High-Throughput Identification of Anthrax-Causing and Emetic Bacillus cereus Group Genome Assemblies via BTyper, a Computational Tool for Virulence-Based Classification of Bacillus cereus Group Isolates by Using Nucleotide Sequencing Data

Rapid, High-Throughput Identification of Anthrax-Causing and Emetic Bacillus cereus Group Genome Assemblies via BTyper, a Computational Tool for Virulence-Based Classification of Bacillus cereus Group Isolates by Using Nucleotide Sequencing Data

Genome Sequence of Bacillus cereus FORC_021, a Food-Borne Pathogen Isolated from a Knife at a Sashimi Restaurant

Genome sequences published outside of Standards in Genomic Sciences, July - October 2012

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