Complete Genome Sequence of an H10N8 Avian Influenza Virus Isolated from a Live Bird Market in Southern China

Jiao, Peirong; Cao, Lingfeng; Yuan, Runyu; Wei, Liangmeng; Song, Yafen; Shen, Dan; Gong, Lang; Luo, Kun; Ren, Tao; Liao, Ming

doi:10.1128/jvi.00959-12

Cited by 33 publications

(28 citation statements)

References 11 publications

(4 reference statements)

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“…More importantly, these seroepidemiological Table 1 Characteristics of study subjects upon enrollment, Guangdong China, 2013 findings are corroborated by our previous epidemiologic study which suggested that H10N8 was recently circulating among poultry in Guangdong Province [3]. On the other hand, the evidence of infection involved only three of the 827 study subjects and all had relatively low HI and MN antibody titers (1:40 or 1:80).…”

Section: Discussionsupporting

confidence: 67%

Antibodies against H10N8 avian influenza virus among animal workers in Guangdong Province before November 30, 2013, when the first human H10N8 case was recognized

Xiao

et al. 2014

BMC Medicine

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Background: Considered an epicenter of pandemic influenza virus generation, southern China has recently seen an increasing number of human H7N9 infections. However, it is not the only threat. On 30 November 2013, a human H10N8 infection case was first described in China. The origin and genetic diversity of this novel virus is similar to that of H7N9 virus. As H10N8 avian influenza virus (AIV) was first identified from a duck in Guangdong Province during 2012 and there is also evidence of H10N8 infected dogs in this region, we sought to examine archived sera from animal workers to see if there was evidence of subclinical human infections before the first human H10N8 cases. Methods:We studied archived serum samples (cross-sectional study, convenience sample) collected between May and September 2013 from 710 animal workers and 107 non-animal exposed volunteers living in five cities of Guangdong Province. Study participants' sera were tested by horse red blood cells (RBCs) hemagglutination inhibition (HI) and microneutralization (MN) assays according to World Health Organization guidelines. The A/Jiangxi-Donghu/346-1/2013 (H10N8) virus was used. Sera which have an HI assay ≥1:20 were further tested with the MN assay. Questionnaire data were examined for risk factor associations with positive serological assays. Risk factor analyses failed to identify specific factors associated with probable H10N8 infections. Results: Among the 827 sera, only 21 animal workers had an HI titer ≥1:20 (18 had an HI titer of 1:20 and 3 had an HI titer of 1:40). None of these 21 subjects reported experiencing any influenza symptoms during the three months before enrollment. Among the three subjects with HI titers of 1:40, two had MN antibody titers of 1:40, and one had a MN antibody titer of 1:80 (probable H10N8 infections).Conclusions: Study data suggest that animal workers may have been infected with the H10N8 virus before the first recognized H10N8 human infection cases. It seems prudent to continue surveillance for H10N8 viruses among animal workers.

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Section: Discussionsupporting

confidence: 67%

Antibodies against H10N8 avian influenza virus among animal workers in Guangdong Province before November 30, 2013, when the first human H10N8 case was recognized

Xiao

et al. 2014

BMC Medicine

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“…Thus, the coexistence of H10, H9, and N8 as well as H10 and N8 in chickens suggested that chickens might serve as an intermediate host for H10N8 virus reassortment in LPMs and might be the major source of transmission of the influenza virus to humans in Nanchang. The novel H10N8 virus obtained from the first patient (6) was significantly different from the other H10N8 viruses, isolated from a Dongting lake wetland (18) and a live bird market in Guangdong Province (19), which leads to the speculation that the H10N8 virus has a stronger infectivity in chickens and is adapted for infection of humans. Since the H9N2 virus can be shed from healthy infected chickens for 8 days and continues to infect exposed chickens (20), the relationship should be studied further to understand the mechanism of how AIVs, including the H10N8 virus, infect humans.…”

Section: Discussionmentioning

confidence: 97%

Coexistence of Avian Influenza Virus H10 and H9 Subtypes among Chickens in Live Poultry Markets during an Outbreak of Infection with a Novel H10N8 Virus in Humans in Nanchang, China

et al. 2015

Jpn J Infect Dis

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SUMMARY:Infection with the novel H10N8 virus in humans has raised concerns about its pandemic potential worldwide. We report the results of a cross-sectional study of avian influenza viruses (AIVs) in live poultry markets (LPMs) in Nanchang, China, after the first human case of H10N8 virus infection was reported in the city. A total of 201 specimens tested positive for AIVs among 618 samples collected from 24 LPMs in Nanchang from December 2013 to January 2014. We found that the LPMs were heavily contaminated by AIVs, with H9, H10, and H5 being the predominant subtypes and more than half of the LPMs providing samples that were positive for the H10 subtype. Moreover, the coexistence of different subtypes was common in LPMs. Of the 201 positive samples, 20.9z (42/201) had mixed infections with AIVs of different HA subtypes. Of the 42 mixed infections, 50z (21/42) showed the coexistence of the H9 and H10 subtypes, with or without H5, and were from chicken samples. This indicated that the H10N8 virus probably originated from segment reassortment of the H9 and H10 subtypes.

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“…Moreover, internal genes of A(H9N2) were found in A(H10N8), A(H7N9) and A(H5N1), showing that A(H9N2) can reassort with other influenza subtypes, potentially resulting in the generation of new zoonotic influenza types [140-142]. In 2013, human cases of infection with H10N8 and H6N1 were reported, as well as presence of these serotypes in environmental samples from animal markets, showing that H6 and H10 serotypes can likewise pose a risk to human health [140,143-145]. Serological cohort studies of persons exposed to poultry provide information on the incidence and longevity of antibodies to zoonotic influenza viruses.…”

Section: Discussionmentioning

confidence: 99%

Weighing serological evidence of human exposure to animal influenza viruses − a literature review

2016

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Assessing influenza A virus strains circulating in animals and their potential to cross the species barrier and cause human infections is important to improve human influenza surveillance and preparedness. We reviewed studies describing serological evidence of human exposure to animal influenza viruses. Comparing serological data is difficult due to a lack of standardisation in study designs and in laboratory methods used in published reports. Therefore, we designed a scoring system to assess and weigh specificity of obtained serology results in the selected articles. Many studies report reliable evidence of antibodies to swine influenza viruses among persons occupationally exposed to pigs. Most avian influenza studies target H5, H7 and H9 subtypes and most serological evidence of human exposure to avian influenza viruses is reported for these subtypes. Avian influenza studies receiving a low grade in this review often reported higher seroprevalences in humans compared with studies with a high grade. Official surveillance systems mainly focus on avian H5 and H7 viruses. Swine influenza viruses and avian subtypes other than H5 and H7 (emphasising H9) should be additionally included in official surveillance systems. Surveillance efforts should also be directed towards understudied geographical areas, such as Africa and South America.

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Complete Genome Sequence of an H10N8 Avian Influenza Virus Isolated from a Live Bird Market in Southern China

Cited by 33 publications

References 11 publications

Antibodies against H10N8 avian influenza virus among animal workers in Guangdong Province before November 30, 2013, when the first human H10N8 case was recognized

Antibodies against H10N8 avian influenza virus among animal workers in Guangdong Province before November 30, 2013, when the first human H10N8 case was recognized

Coexistence of Avian Influenza Virus H10 and H9 Subtypes among Chickens in Live Poultry Markets during an Outbreak of Infection with a Novel H10N8 Virus in Humans in Nanchang, China

Weighing serological evidence of human exposure to animal influenza viruses − a literature review

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