Complete genome sequence of an extensively drug resistant (XDR) M. morganii SMM01 isolated from a patient with urinary and fecal incontinence

Abstract

Objective M. morganii is a gram-negative, non-lactose fermenting and an opportunistic pathogen frequently associated with nosocomial infections. Although first isolated in 1906 from a pediatric fecal sample, not many M. morganii isolates have been sequenced. The objective of this work is to determine the complete genome sequence of an XDR M. morganii strain (SMM01) isolated from the urine of a patient with urinary and fecal incontinence and to characterize its antimicrobial resistance profile. Data description Here, we report the complete genome sequence of M. morganii SMM01 generated from the hybrid assembly of Illumina HiSeq X and Nanopore MinION reads. The assembly is 100% complete with genome size of 39,30,130 bp and GC content of 51%. Genomic features include 3617 CDS, 18 rRNAs, 78 tRNAs, 4 ncRNAs and 60 pseudogenes. Antimicrobial resistance profile was characterized by the presence of genes conferring resistance to aminoglycosides, β-lactams, fluoroquinolones, chloramphenicol, and tetracyclines. Secondary metabolite biosynthetic gene clusters like NRPS, T1PKS, thiopeptide, beta-lactone, and bacteriocin were identified. The genome data described here would be the first complete genome of an Indian M. morganii isolate providing crucial information on antimicrobial resistance patterns, paving the way for further comparative genome analyses.

Genomic sequencing should extend to diverse priority pathogens for effective study and surveillance of antimicrobial resistance: a systematic review of whole-genome sequencing studies from India

“…130 Similarly, for E. cloacae we found only two studies that reported the total of two whole genome sequences, while for M. morganii and K. aerogenes , we found one study with a single sequence each. 132–135…”

“…130 Similarly, for E. cloacae we found only two studies that reported the total of two whole genome sequences, while for M. morganii and K. aerogenes, we found one study with a single sequence each. [132][133][134][135] We also looked at the geographic distribution of the whole-genome sequences across the six different geo-climatic zones of India (Figure 2). Out of the 2547 sequences, we could locate the geo-climatic zones of 1653 sequences (64.90% of the sequences) based on the information in the reported study or from the NCBI metadata associated with the genomic sequence.…”

“…130 Similarly, for E. cloacae we found only two studies that reported the total of two whole genome sequences, while for M. morganii and K. aerogenes, we found one study with a single sequence each. [132][133][134][135] . CC-BY-NC-ND 4.0 International license available under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.…”