Complete Genome Sequence of an African Raw Honey Bacterial Isolate, Bacillus safensis Strain AHB11

Shin, Carson J; O’Connor, Tamara J.

doi:10.1128/mra.00516-22

Cited by 1 publication

(2 citation statements)

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“…16S sequencing of AHB2 and AHB11 indicated they are both members of the Bacillus genus ( Table S2 ). Subsequent whole genome sequencing of AHB2 ( 47 ) and AHB11 ( 48 ) further defined these bacteria as Bacillus safensis , based on multi-locus sequencing typing of 16S, 23S, rpoB , gyrB , and recA ( Table S2 ). However, with only 90% gene conservation between them and a set of 732 genes unique to one or the other, they represent two distinct strains.…”

Section: Resultsmentioning

confidence: 99%

“…Assembly was performed with Unicycler ( 76 ), and assembly statistics were assessed with QUAST ( 77 ). The genomes were annotated by National Center for Biotechnology Information using Prokaryotic Genome Annotation Pipeline ( 78 ) and deposited in GenBank with accession numbers CP097373 for AHB2 ( 47 ) and CP097374 and CP097375 for AHB11 ( 48 ).…”

Section: Methodsmentioning

confidence: 99%

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Novel induction of broad-spectrum antibiotics by the human pathogen Legionella

Shin,

O'Connor

2024

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The majority of antibiotics are natural products, with microorganism-generated molecules and their derivatives being the most prevalent source of drugs to treat infections. Thus, identifying natural products remains the most valuable resource for novel therapeutics. Here, we report the discovery of a series of dormant bacteria in honey that have bactericidal activity toward Legionella , a bacterial pathogen that causes respiratory disease in humans. We show that, in response to bacterial products secreted by Legionella , the honey bacteria release diffusible antimicrobial molecules. Remarkably, the honey bacteria only produce these molecules in response to Legionella spp., when compared to a panel of 24 bacterial pathogens from different genera. However, the molecules induced by Legionella have broad activity against several clinically important pathogens, including many high-priority pathogens. Thus, Legionella spp. are potent drivers of antimicrobial molecule production by uncharacterized bacteria isolated from honey, providing access to new antimicrobial products and an unprecedented strategy for discovering novel antibiotics. IMPORTANCE Natural products generated by microorganisms remain the most viable and abundant source of new antibiotics. However, their discovery depends on the ability to isolate and culture the producing organisms and to identify conditions that promote antibiotic production. Here, we identify a series of previously undescribed bacteria isolated from raw honey and specific culture conditions that induce the production of antimicrobial molecules that are active against a wide variety of pathogenic bacteria.

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Section: Resultsmentioning

confidence: 99%