Complete Genome Sequence of a Novel Monopartite Mastrevirus, Soybean Geminivirus B, Isolated from Soybean (Glycine max (L.) Merrill)

Abstract

Soybean is one of the most important crops in Korea. To identify the viruses infecting soybean, we conducted RNA sequencing with samples displaying symptoms of viral disease. A contig displaying sequence similarity to the known Geminivirus was identified. A polymerase chain reaction (PCR) using two different pairs of back-to-back primers and rolling circle amplification (RCA) confirmed the complete genome of a novel virus named soybean geminivirus B (SGVB), consisting of a circular monopartite DNA genome measuring 2616 nucleotides (nt) in length. SGVB contains four open reading frames (ORFs) and three intergenic regions (IRs). IR1 includes a nonanucleotide origin of replication in the stem-loop structure. Phylogenetic and BLAST analyses demonstrated that SGVB could be a novel virus belonging to the genus Mastrevirus in the family Geminiviridae. We generated infectious clones for SGVB by adding a copy of the IR1 region of SGVB, comparing the V-ori in addition to the full-length genome of SGVB. Using the infectious clones, we observed chlorosis and leaf curling with a latent infection in the inoculated Nicotiana benthamiana plants, while none of the inoculated soybean plants showed any visible symptoms of disease. This study provides the complete genome sequence and infectious clones of a novel Mastrevirus referred to as SGVB from soybean in Korea.

Investigating Variability in Viral Presence and Abundance across Soybean Seed Development Stages Using Transcriptome Analysis

“…In addition to SMV, several other RNA viruses have been identified in soybean, including soybean vein necrosis-associated virus (SVNaV), bean pod mottle virus (BPMV), soybean yellow mottle mosaic virus (SYMMV), soybean yellow common mosaic virus (SYCMV), peanut stunt virus (PSV), peanut mottle virus (PeMoV), and alfalfa mosaic virus (AMV) [ 2 , 3 ]. Furthermore, recent studies have uncovered the presence of novel geminiviruses infecting soybean; however, the disease symptoms caused by these geminiviruses in soybean plants are largely asymptomatic [ 9 , 10 ]. These viruses, along with their variants and strains, exhibit diverse geographic distributions and host ranges, highlighting the global nature of the soybean virome.…”