Complete genome sequence of a novel velarivirus infecting areca palm in China

Yu, Gino; Qi, Shuishui; Chang, Zhaoxia; Rong, Qiqi; Akinyemi, Ibukun A.; Wu, Qingfa

doi:10.1007/s00705-015-2489-9

Cited by 22 publications

(20 citation statements)

References 16 publications

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“…According to Wang et al (2020), they could not find any phytoplasma in YLD infected plantations from different epidemic areas in Hainan. However, based on RNA-seq (Yu et al, 2015), nextgeneration sequencing, immuno-capture RT-PCR and immunofluorescence assays (Zhang et al, 2021), and RNA-seq and de novo assembly (Cao et al, 2021), it was concluded that APV1 is the causal agent of YLD in Hainan, China. The APV1 belongs to the family Closteroviridae, having a flexuous and filamentous shape observed under transmission electron microscopy (TEM), the typical morphology of species in the family Closteroviridae (Wang et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…The APV1 belongs to the family Closteroviridae, having a flexuous and filamentous shape observed under transmission electron microscopy (TEM), the typical morphology of species in the family Closteroviridae (Wang et al, 2020). The genome of APV1-HN associated with YLD from Hainan, China was first sequenced through RNA-seq and was 16,080 nt in length (Yu et al, 2015), while the complete genome sequence of APV1-WYN (Wanning) isolate was approximately 1.5 kb longer than the APV1-HN genome and was recorded to be 17,546 nt in length. In our previously published data regarding APV1 genetic diversity throughout Hainan (China), 20 new complete genomes of APV1 isolates have been identified and sequenced through RNA-seq and de novo assembly and were divided into three phylogroups based on phylogenetic analysis, in which phylogroup A is the most prominent group (>70%), which consists of 16 isolates (Cao et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Religious and masticatory purposes make their significance more unique (Kurian and Peter, 2007). The areca palm plantation is under attack by different pathogens, including bacteria (Kumar, 1983;Nair et al, 2016), fungi (To-anun et al, 2009;Wang et al, 2019), insects such as Tirathaba rufivena (Zhong et al, 2017) and Brontispa longissima (Li et al, 2007), phytoplasmas (Ramaswamy et al, 2013;Kanatiwela de Silva et al, 2015), and viruses (Yu et al, 2015;Yang et al, 2018a;Yang et al, 2018b;Wang et al, 2020;Cao et al, 2021;Zhang et al, 2022). The approved causal agent of YLD specifically in Hainan, China is the novel virus named areca palm velarivirus 1 (APV1) based on de novo assembly, RNA-seq, and next-generation sequencing (Yu et al, 2015;Wang et al, 2020;Cao et al, 2021), while the responsible transmission vectors of APV1 in Hainan are mealybugs Pseudococcus cryptus and Ferrisia virgata on the basis of immunocapture RT-PCR and immunofluorescence assays (Zhang et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…The areca palm plantation is under attack by different pathogens, including bacteria (Kumar, 1983;Nair et al, 2016), fungi (To-anun et al, 2009;Wang et al, 2019), insects such as Tirathaba rufivena (Zhong et al, 2017) and Brontispa longissima (Li et al, 2007), phytoplasmas (Ramaswamy et al, 2013;Kanatiwela de Silva et al, 2015), and viruses (Yu et al, 2015;Yang et al, 2018a;Yang et al, 2018b;Wang et al, 2020;Cao et al, 2021;Zhang et al, 2022). The approved causal agent of YLD specifically in Hainan, China is the novel virus named areca palm velarivirus 1 (APV1) based on de novo assembly, RNA-seq, and next-generation sequencing (Yu et al, 2015;Wang et al, 2020;Cao et al, 2021), while the responsible transmission vectors of APV1 in Hainan are mealybugs Pseudococcus cryptus and Ferrisia virgata on the basis of immunocapture RT-PCR and immunofluorescence assays (Zhang et al, 2022). The most conspicuous symptom of YLD is the yellowing of the leaves; the symptoms emergence that was observed and recorded in the present research work was the same as those described by Wang et al (2020) and Zhang et al (2022), although the severity of the symptoms towards different temperatures and seasonal effects was not known exactly before, and was needed to be explored.…”

Section: Introductionmentioning

confidence: 99%

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Effect of temperature on yellow leaf disease symptoms and its associated areca palm velarivirus 1 titer in areca palm (Areca catechu L.)

et al. 2022

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Yellow leaf disease (YLD) has been a major limiting factor threatening areca palm commonly known as betel palm (Areca catechu L.) plantations in Hainan, China. The YLD disease is closely associated with areca palm velarivirus 1 (APV1), which belongs to the family Closteroviridae. YLD-affected betel palms show more serious yellowing symptoms in winter than in summer based on anecdotal observations. In the present work, the underlying mechanism was investigated. We first observed that the severity of YLD symptoms was closely related with the APV1 viral titer determined by qRT-PCR and ELISA under natural conditions. To further investigate whether temperature plays a key role in APV1 accumulation, the areca palm seedlings were artificially inoculated with APV1-positive mealybugs (Ferrisia virgata) and then cultivated under controlled conditions. According to our results, the YLD symptoms severity in inoculated seedlings were closely associated with temperature, e.g., severest symptoms at low temperature (16/22 ± 2°C, night/day), severer symptoms at room temperature (24/26 ± 2°C, night/day), while moderate symptoms at high temperature (27/34 ± 2°C, night/day). The qRT-PCR and ELISA results showed that APV1 titer accumulates significantly abundant at low temperature as compared to high and room temperatures. In conclusion, this is the first report about the temperature effects on the symptoms severity of YLD and APV1 titer, which may have important implications for the epidemiology of YLD.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of temperature on yellow leaf disease symptoms and its associated areca palm velarivirus 1 titer in areca palm (Areca catechu L.)

et al. 2022

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“…Although the production of arecanut is confined to a few states, commercial products are extensively exported across the globe, and approximately 700 million people are dependent on arecanut worldwide [7,8]. Being a perennial crop, arecanut is highly vulnerable to numerous pathogens, including fungi [9][10][11], bacteria [12], phytoplasma [13][14][15], and viruses [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Spatial Distribution and Risk Associated with Fruit Rot Disease in Areca catechu L.

Patil

Sridhara

Shil

et al. 2021

JoF

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Phytophthora meadii (McRae) is a hemibiotrophic oomycete fungus that infects tender nuts, growing buds, and crown regions, resulting in fruit, bud, and crown rot diseases in arecanut (Areca catechu L.), respectively. Among them, fruit rot disease (FRD) causes serious economic losses that are borne by the growers, making it the greatest yield-limiting factor in arecanut crops. FRD has been known to occur in traditional growing areas since 1910, particularly in Malnad and coastal tracts of Karnataka. Systemic surveys were conducted on the disease several decades ago. The design of appropriate management approaches to curtail the impacts of the disease requires information on the spatial distribution of the risks posed by the disease. In this study, we used exploratory survey data to determine areas that are most at risk. Point pattern (spatial autocorrelation and Ripley’s K function) analyses confirmed the existence of moderate clustering across sampling points and optimized hotspots of FRD were determined. Geospatial techniques such as inverse distance weighting (IDW), ordinary kriging (OK), and indicator kriging (IK) were performed to predict the percent severity rates at unsampled sites. IDW and OK generated identical maps, whereby the FRD severity rates were higher in areas adjacent to the Western Ghats and the seashore. Additionally, IK was used to identify both disease-prone and disease-free areas in Karnataka. After fitting the semivariograms with different models, the exponential model showed the best fit with the semivariogram. Using this model information, OK and IK maps were generated. The identified FRD risk areas in our study, which showed higher disease probability rates (>20%) exceeding the threshold level, need to be monitored with the utmost care to contain and reduce the further spread of the disease in Karnataka.

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Grapevine leafroll-associated virus 7

Rwahnih

Saldarelli

Rowhani

2017

Grapevine Viruses: Molecular Biology, Diagnostics and Management

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Grapevine leafroll disease (GLD) is one of the most important grapevine viral diseases affecting grapevines worldwide. The impact on vine health, crop yield, and quality is difficult to assess due to a high number of variables, but significant economic losses are consistently reported over the lifespan of a vineyard if intervention strategies are not implemented. Several viruses from the family Closteroviridae are associated with GLD. However, Grapevine leafroll-associated virus 3 (GLRaV-3), the type species for the genus Ampelovirus, is regarded as the most important causative agent. Here we provide a general overview on various aspects of GLRaV-3, with an emphasis on the latest advances in the characterization of the genome. The full genome of several isolates have recently been sequenced and annotated, revealing the existence of several genetic variants. The classification of these variants, based on their genome sequence, will be discussed and a guideline is presented to facilitate future comparative studies. The characterization of sgRNAs produced during the infection cycle of GLRaV-3 has given some insight into the replication strategy and the putative functionality of the ORFs. The latest nucleotide sequence based molecular diagnostic techniques were shown to be more sensitive than conventional serological assays and although ELISA is not as sensitive it remains valuable for high-throughput screening and complementary to molecular diagnostics.The application of next-generation sequencing is proving to be a valuable tool to study the complexity of viral infection as well as plant pathogen interaction. Next-generation sequencing data can provide information regarding disease complexes, variants of viral species, and abundance of particular viruses. This information can be used to develop more accurate diagnostic assays. Reliable virus screening in support of robust grapevine certification programs remains the cornerstone of GLD management.

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Complete genome sequence of a novel velarivirus infecting areca palm in China

Cited by 22 publications

References 16 publications

Effect of temperature on yellow leaf disease symptoms and its associated areca palm velarivirus 1 titer in areca palm (Areca catechu L.)

Effect of temperature on yellow leaf disease symptoms and its associated areca palm velarivirus 1 titer in areca palm (Areca catechu L.)

Assessment of the Spatial Distribution and Risk Associated with Fruit Rot Disease in Areca catechu L.

Grapevine leafroll-associated virus 7

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