Complete genome sequence of a keratin-degrading bacterium Chryseobacterium gallinarum strain DSM 27622T isolated from chicken

Park, Gun Seok; Hong, Sung Jun; Jung, Byung Kwon; Khan, Abdur Rahim; Park, Yeong Jun; Park, Chang Eon; Lee, Ara; Kwak, Yunyoung; Lee, Yong Jik; Lee, Dong Woo; Lee, Changhee; Park, Choi Kyu; Shin, Jae Ho

doi:10.1016/j.jbiotec.2015.07.007

Cited by 11 publications

(7 citation statements)

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“…Including: (i) Chryseobacterium camelliae Dolsongi-HT1, isolated from green tea leaves [ 48 ]; (ii) Chryseobacterium gallinarum strain DSM 27622, isolated from chicken [ 49 ]; and (iii) Chryseobacterium sp. P1-3 isolated from poultry waste [ 50 ], which all display keratinolytic capacity.…”

Section: Resultsmentioning

confidence: 99%

Comparative Genomics Analysis of Keratin-Degrading Chryseobacterium Species Reveals Their Keratinolytic Potential for Secondary Metabolite Production

et al. 2021

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A promising keratin-degrading strain from the genus Chryseobacterium (Chryseobacterium sp. KMC2) was investigated using comparative genomic tools against three publicly available reference genomes to reveal the keratinolytic potential for biosynthesis of valuable secondary metabolites. Genomic features and metabolic potential of four species were compared, showing genomic differences but similar functional categories. Eleven different secondary metabolite gene clusters of interest were mined from the four genomes successfully, including five common ones shared across all genomes. Among the common metabolites, we identified gene clusters involved in biosynthesis of flexirubin-type pigment, microviridin, and siderophore, showing remarkable conservation across the four genomes. Unique secondary metabolite gene clusters were also discovered, for example, ladderane from Chryseobacterium sp. KMC2. Additionally, this study provides a more comprehensive understanding of the potential metabolic pathways of keratin utilization in Chryseobacterium sp. KMC2, with the involvement of amino acid metabolism, TCA cycle, glycolysis/gluconeogenesis, propanoate metabolism, and sulfate reduction. This work uncovers the biosynthesis of secondary metabolite gene clusters from four keratinolytic Chryseobacterium species and shades lights on the keratinolytic potential of Chryseobacterium sp. KMC2 from a genome-mining perspective, can provide alternatives to valorize keratinous materials into high-value bioactive natural products.

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Section: Resultsmentioning

confidence: 99%

Comparative Genomics Analysis of Keratin-Degrading Chryseobacterium Species Reveals Their Keratinolytic Potential for Secondary Metabolite Production

et al. 2021

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“…( 52 ) and Chryseobacterium sp. ( 53 ). Descriptions of thermophilic and extremophilic keratinolytic representatives from genera Fervidobacterium sp.…”

Section: Microbial Sources Of Keratinasesmentioning

confidence: 99%

Microbial keratinases: enzymes with promising biotechnological 4 applications

Vidmar

Vodovnik

2018

Food Technol. Biotechnol.

103

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SUMMARYKeratin is a complex and structurally stable protein found in human and animal hard tissues, such as feathers, wool, hair, hoof and nails. Some of these, like feathers and wool, represent one of the main sources of protein-rich waste with significant potential to be transformed into value-added products such as feed, fertilizers or bioenergy. A major limitation impeding valorization of keratinous substrates is their recalcitrant structure and resistance to hydrolysis by common proteases. However, specialized keratinolytic enzymes produced by some microorganisms can efficiently degrade these substrates. Keratinases have already found a purpose in pharmaceutical, textile and leather industries. However, their wider implementation in other processes, such as cost-effective (pre)treatment of poultry waste, still requires optimization of production and performance of the available enzymes. Here we present a comprehensive review covering molecular properties and characteristics of keratinases, their classification, traditional and novel approaches in discovery of novel enzymes, production, characterization, improvement and biotechnological applications.

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“…KMC2 was sequenced and compared to three reference genomes of Chryseobacterium spp. Including: i) Chryseobacterium camelliae Dolsongi-HT, isolated from green tea leaves (29); ii) Chryseobacterium gallinarum strain DSM 27622, isolated from chicken (30); and iii) Chryseobacterium sp. strain P1-3 isolated from poultry waste (31), which all display keratinolytic capacity (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Comparative genomics analysis of Chryseobacterium sp. KMC2 reveals metabolic pathways involved in keratinous utilization and natural product biosynthesis

Kang

Shoaie

Jacquiod

et al. 2021

Preprint

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Several efforts have been made to valorize keratinous materials, an abundant and renewable resource. Despite these attempts to valorize products generated from keratin hydrolysate, either via chemical or microbial conversion, they generally remain with an overall low value. In this study, a promising keratinolytic strain from the genus Chryseobacterium (Chryseobacteriumsp. KMC2) was investigated using comparative genomic tools against publicly available reference genomes to reveal the metabolic potential for biosynthesis of valuable secondary metabolites. Genome and metabolic features of four species were compared, shows different gene numbers but similar functional categories. We successfully mined eleven different secondary metabolite gene clusters of interest from the four genomes, including five common ones shared across all genomes. Among the common metabolites, we identified gene clusters involved in biosynthesis of flexirubin-type pigment, microviridin, and siderophore, all showing remarkable conservation across the four genomes. Unique secondary metabolite gene clusters were also discovered, for example, ladderane from Chryseobacterium sp. KMC2. Additionally, this study provides a more comprehensive understanding of the potential metabolic pathways of keratin utilization in Chryseobacterium sp. KMC2, with the involvement of amino acid metabolism, TCA cycle, glycolysis/gluconeogenesis, propanoate metabolism, and sulfate reduction. This work uncovers the biosynthesis of secondary metabolite gene clusters from four keratinolytic Chryseobacterium spp. and shades lights on the keratinolytic potential of Chryseobacterium sp. KMC2 from a genome-mining perspective, providing alternatives to valorize keratinous materials into high-value natural products.

show abstract

Complete genome sequence of a keratin-degrading bacterium Chryseobacterium gallinarum strain DSM 27622T isolated from chicken

Cited by 11 publications

References 11 publications

Comparative Genomics Analysis of Keratin-Degrading Chryseobacterium Species Reveals Their Keratinolytic Potential for Secondary Metabolite Production

Comparative Genomics Analysis of Keratin-Degrading Chryseobacterium Species Reveals Their Keratinolytic Potential for Secondary Metabolite Production

Microbial keratinases: enzymes with promising biotechnological 4 applications

Comparative genomics analysis of Chryseobacterium sp. KMC2 reveals metabolic pathways involved in keratinous utilization and natural product biosynthesis

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