Complete genome sequence of a novel nege-like virus in aphids (genus Indomegoura)

Qi, Yajuan; Xu, Liao-Yuan; Zhai, Jing; Ye, Zhuang-Xin; Lu, Gang; Chen, Jianping; Zhang, Chuan‐Xi; Li, Junmin

doi:10.1186/s12985-021-01552-w

Cited by 7 publications

(11 citation statements)

References 22 publications

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“…The assembly of the 11.7 kb contig was accomplished from 3,865 reads, with an average sequencing depth of 34X. A significantly higher read count was identified at the 3’ end ( Fig 1 ), as recently observed in Indomegoura nege‐like virus 1 (INLV1) [ 14 ] and Astegopteryx formosana nege-like virus (AFNLV) [ 42 ]. This study focuses on the genomic analysis of this novel negevirus-like sequence, hereby designated as whitefly negevirus 1 (WfNgV1).…”

Section: Resultsmentioning

confidence: 66%

Analysis of a new negevirus-like sequence from Bemisia tabaci unveils a potential new taxon linking nelorpi- and centiviruses

Quito-Avila,

Reyes-Proaño,

Armijos-Capa

et al. 2024

PLoS ONE

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This study presents the complete genome sequence of a novel nege-like virus identified in whiteflies (Bemisia tabaci MEAM1), provisionally designated as whitefly negevirus 1 (WfNgV1). The virus possesses a single-stranded RNA genome comprising 11,848 nucleotides, organized into four open reading frames (ORFs). These ORFs encode the putative RNA-dependent-RNA-polymerase (RdRp, ORF 1), a glycoprotein (ORF 2), a structural protein with homology to those in the SP24 family, (ORF 3), and a protein of unknown function (ORF 4). Phylogenetic analysis focusing on RdRp and SP24 amino acid sequences revealed a close relationship between WfNgV1 and Bemisia tabaci negevirus 1, a negevirus sequence recently discovered in whiteflies from Israel. Both viruses form a clade sharing a most recent common ancestor with the proposed nelorpivirus and centivirus taxa. The putative glycoprotein from ORF 2 and SP24 (ORF 3) of WfNgV1 exhibit the characteristic topologies previously reported for negevirus counterparts. This marks the first reported negevirus-like sequence from whiteflies in the Americas.

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Section: Resultsmentioning

confidence: 66%

Analysis of a new negevirus-like sequence from Bemisia tabaci unveils a potential new taxon linking nelorpi- and centiviruses

Quito-Avila,

Reyes-Proaño,

Armijos-Capa

et al. 2024

PLoS ONE

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“…To solve this issue, exploring RNAi approaches using endogenous aphid viruses that can be vertically transmitted to progeny as VIGS vectors may be a more stable approach. Next-generation sequencing has identified many potential aphid viruses (Kondo et al 2020;Chang et al 2021;Qi…”

Section: Discussionmentioning

confidence: 99%

Flock house virus as a vehicle for aphid Virus-induced gene silencing and a model for aphid biocontrol approaches

et al. 2022

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Due to their high specificity and efficacy, RNA interference (RNAi)-based strategies have been used for fundamental functional genomics studies in a number of insects. However, its potential for translational applications in pest management is also of great interest. The lack of suitable RNAi triggering approaches, however, so far has largely precluded the implementation of RNAi-based approaches to target aphids. In this work, we first demonstrate that Flock House virus (FHV), an insect virus, can infect multiple aphid species, including the green peach aphid, Myzus persicae (M. persicae), the corn leaf aphid, Rhopalosiphum maidis (R. maidis), and the bird cherry-oat aphid, Rhopalosiphum padi (R. padi), by both microinjection and oral feeding. Using green fluorescent protein (GFP) as an indicator, we showed that the defective interfering RNA (DI-634) of FHV RNA2, which is generated autonomously during wild-type (WT) virus replication, can carry foreign sequences, and further for their functional expression. More importantly, the engineered DI-634 was incorporated into virus particles in co-infections with WT FHV. Using FHV virions containing genetically modified DI-634, the accumulation levels of the M. persicae mRNAs for Cathepsin L (CatL) and Sugar Transporter 4 (ST4), were decreased by ~ 35% and ~ 30–50%, respectively when virions were injected intrathoracically into aphids. Finally, and of more practical relevance, oral acquisition of these engineered FHV virions caused lethality of M. persicae. In summary, as a proof-of-concept, our work demonstrates that FHV can be a valuable RNAi tool for fundamental research, and suggests opportunities for using engineered insect viruses as biological agents for aphid pest control.

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“…(Turkana_9) [174]. Furthermore, other negeviruses were detected in the dungfly (Scathophaga furcata) by next-generation sequencing, and in various members of Insecta, Arachnida, Malacostraca, Maxillopoda, Chilopoda, Nematoda and Cnidaria by searching various bioinformatics databases [175,176].…”

Section: Negevirus (Ictv Unclassified)mentioning

confidence: 99%

The role of sand flies as vectors of viruses other than phleboviruses

Jancarova

Polanska

Volf

et al. 2023

Journal of General Virology

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Sand flies (Diptera: Phlebotominae) are proven vectors of various pathogens of medical and veterinary importance. Although mostly known for their pivotal role in the transmission of parasitic protists of the genus Leishmania that cause leishmaniases, they are also proven or suspected vectors of many arboviruses, some of which threaten human and animal health, causing disorders such as human encephalitis (Chandipura virus) or serious diseases of domestic animals (vesicular stomatitis viruses). We reviewed the literature to summarize the current published information on viruses detected in or isolated from phlebotomine sand flies, excluding the family Phenuiviridae with the genus Phlebovirus, as these have been well investigated and up-to-date reviews are available. Sand fly-borne viruses from four other families (Rhabdoviridae, Flaviviridae, Reoviridae and Peribunyaviridae) and one unclassified group (Negevirus) are reviewed for the first time regarding their distribution in nature, host and vector specificity, and potential natural transmission cycles.

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Complete genome sequence of a novel nege-like virus in aphids (genus Indomegoura)

Cited by 7 publications

References 22 publications

Analysis of a new negevirus-like sequence from Bemisia tabaci unveils a potential new taxon linking nelorpi- and centiviruses

Analysis of a new negevirus-like sequence from Bemisia tabaci unveils a potential new taxon linking nelorpi- and centiviruses

Flock house virus as a vehicle for aphid Virus-induced gene silencing and a model for aphid biocontrol approaches

The role of sand flies as vectors of viruses other than phleboviruses

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