Complete genome sequence of a polyvalent bacteriophage, phiKP26, active on Salmonella and Escherichia coli

Abstract

Bacteriophages are viruses that specifically infect and lyse prokaryotic cells and therefore might be used as biocontrol agents. However, it is necessary to acquire genomic information to predict and understand the phage's characteristics for the efficient and safe use of bacteriophages as biocontrol agents against bacterial pathogens. In this study, the complete genome sequence of a novel enterobacteriophage, phiKP26, was determined by pyrosequencing. Genomic analysis of phiKP26 revealed a genome size of 47,285 bp with an overall G + C content of 44.3 %. Seventy-eight open reading frames (ORFs) in the phiKP26 genome were grouped into the modules of replication, DNA packaging, morphogenesis, cell lysis and absence of genes related to virulence and lysogeny.

Four Escherichia coli O157:H7 Phages: A New Bacteriophage Genus and Taxonomic Classification of T1-Like Phages

“…This was also confirmed by the phylogenetic analysis of four key functional phage proteins. The fact that the viruses related to JK06 have been isolated independently in Israel (JK06) (GenBank Assession #, NC_007291), Canada (vB_EcoS_Rogue1, AHP24, AHS24, AHP42, AKS96) [40] and Mexico (phiKP26, phiJLA23) [49], [50] between 2005 and 2011 indicates that these similar phages are widely distributed, and that horizontal gene transfer does not always prevent the characterization of bacteriophage evolution. Similar finding have been noted as part of the Phage Hunters Integrating Research and Education (PHIRE) program (http://phagesdb.org/) and for the global distribution of viruses related to Listeria phage A511.…”

“…Similar finding have been noted as part of the Phage Hunters Integrating Research and Education (PHIRE) program (http://phagesdb.org/) and for the global distribution of viruses related to Listeria phage A511. The results indicate that a new subfamily, the “Tunavirinae” created within the family Siphoviridae containing the following genera: a modified Tunalikevirus (T1, ADB-2, Shfl1) [51], [52], “Tlslikevirus” (TLS, FSL SP-126) [51], [53], “Kp36likevirus” (KP36, F20) [54], [55], “Rtplikevirus” (RTP, vB_EcoS_ACG-M12) [56], [57]; and “Jk06likevirus” (JK06, vB_EcoS_Rogue1, AHP24, AHS24, AHP42, AKS96, phiJLA23, phiKP26, phiEB49) [40], [49], [50], [58]. This would leave two putative orphan species: pSf-1 [59] and ESP2949-1 [60] to be further classified as more phages are characterized.…”

HostPhinder: A Phage Host Prediction Tool

“…Although examples exist of phages that have extended their host range based on only a few mutations [ 27 ], the extended host range is typically limited to different strains of the same species. Apart from polyvalent enterobacteria phages, which are able to infect members of phylogenetically linked genera within the Enterobacteriaceae family, e.g., Escherichia , Shigella , and Klebsiella [ 28 , 29 ], most phages have been found to be specific to a particular genus [ 30 ]. This has been indicated by studies examining proteins, not entire proteomes [ 31 ], as has the “Phage Proteomic Tree”, which is based on completely sequenced phage genomes [ 32 ], and analysis of genome type for Mycobacteriophages and host preference [ 33 ].…”

Isolation and Characterization of Bacteriophage ZCSE6 against Salmonella spp.: Phage Application in Milk

“…On the other hand, the ability of phage ZCSE6 to lyse E. coli indicates that the phage can infect two different bacterial genus. Thus, phage ZCSE6 is a "divalent phage", as it has been reported before for Salmonella and E. coli phages [30,31]. Moreover, the host range of phage ZCSE6 solves one of the huge issues of antibiotics, as the narrow host range of bacteriophage reduces the risk of affecting normal microbiota and developing resistance among bacteria.…”