Complete genome sequence of a clinical isolate of Clostridioides difficile bacteriophage phiCDKH01 of the family Siphoviridae

Abstract

A new temperate phiCDKH01 siphophage was obtained from clinical isolate of Clostridioides difficile. The phage genome is a 45,089 bp linear double-stranded DNA molecule with an average G + C content of 28.7%. It shows low similarity to known phage genomes except for phiCD24-1. Genomic and phylogenetic analysis revealed that phiCDKH01 is a novel phage. 66 putative ORFs were predicted in the genome, 37 of which code for proteins with predicted functions. The phiCDKH01 prophage has been localized in the host genome. Results of this study increases genetic diversity of known tailed phages.