Complete Genome Sequence of a Multidrug-Resistant Klebsiella pneumoniae Environmental Isolate from Zanzibar, Tanzania, Harboring Novel Insertion Elements and Two bla _CTX-M-15 Genes

Abstract

Here, we report the annotated whole-genome sequence of Klebsiella pneumoniae strain KP_3b, isolated in Zanzibar, Tanzania, from plastic litter. The strain is extended-spectrum β-lactamase (ESBL) producing and multidrug resistant, encoding 17 resistance genes, most of which are located on a 230,544-bp plasmid. The isolate contains two copies of the bla CTX-M-15 gene and novel insertion elements.

Complete Genome Sequences of Extraintestinal Pathogenic Escherichia coli Clinical Isolates from Danish Ulcerative Colitis Patients

“…Sequencing and postprocessing were performed as described by Lallement et al ( 11 ) using a combined short- and long-read sequencing approach. The read length of long-read sequencing was favored by omitting shearing and size selection of DNA before library preparation using an SQK-LSK109 ligation kit and sequencing on an Oxford Nanopore MinION instrument with a R9.4.1 flow cell.…”