Complete Genome Sequence of a Novel Subgenotype of Porcine Reproductive and Respiratory Syndrome Virus Strain JX/CH/2016, Isolated in Jiangxi, China

Zhang, Fanfan; Song, Deping; Ye, Yu; Guo, Ning; Zhang, Min; Li, Hao; Liu, Dan; Luo, Suxian; Huang, Deng-Shing; Tang, Yuxin

doi:10.1128/genomea.00980-17

Cited by 2 publications

(1 citation statement)

References 10 publications

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“…Moreover, the QYYZ-like strains (sublineage 3.5) were prevalent only in mainland China. According to the results of this study and previous studies, QYYZ-like strains have been occasionally reported in northern China ( 19 , 26 , 28 , 53 ) but are mainly prevalent in southern and central China ( 12 – 16 , 23 – 25 , 27 , 29 , 30 , 32 ). Although recombination of PRRSV is very common, many non-recombination strains of JXA1-like ( 54 ), VR2332-like ( 28 ) and NADC30-like/NADC34-like PRRSV ( 7 , 55 ) have been reported after long-term evolution in China.…”

Section: Resultssupporting

confidence: 78%

Genome-Wide Characterization of QYYZ-Like PRRSV During 2018–2021

Xiang

Tang

et al. 2022

Front. Vet. Sci.

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In the last decade, the emergence of QYYZ-like porcine reproductive and respiratory syndrome virus (PRRSV) has attracted increasing attention due to the high incidence of PRRSV mutation and recombination. However, the endemic status and genomic characteristics of the QYYZ-like strains are unclear. From 2018 to October 2021, 24 QYYZ-like PRRSV isolates were obtained from 787 PRRSV-positive clinical samples. Only one QYYZ-like positive sample was from a northern province, and the rest were from central and southern provinces. We selected 9 samples for whole-genome sequencing, revealing genome lengths of 15,008–15,316 nt. We retrieved all the available whole-genome sequences of QYYZ-like PRRSVs isolated in China from 2010 to 2021 (n = 28) from GenBank and analyzed them together with the new whole-genome sequences (n = 9). Phylogenetic tree analysis based on the ORF5 gene showed that all QYYZ-like PRRSV strains belonged to sublineage 3.5 but were clustered into three lineages (sublineage 1.8, sublineage 3.5, and sublineage 8.7) based on whole-genome sequences. Genomic sequence alignment showed that QYYZ-like strains, have characteristic amino acids insertions or deletions in the Nsp2 region (same as NADC30, JXA1 and QYYZ) and that thirteen strains also had additional amino acid deletions, mostly between 468 and 518 aa. Moreover, QYYZ-like strains (sublineage 3.5) have seven identical characteristic amino acid mutations in ORF5. Recombination analysis revealed that almost all QYYZ-like complete genome sequences (36/37) were products of recombination and mainly provided structural protein fragments (GP2-N) for the recombinant strains. Overall, QYYZ-like strains were mainly prevalent in central and southern China from 2018 to 2021, and these strains provided recombinant fragments in the PRRSV epidemic in China.

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Section: Resultssupporting

confidence: 78%

Genome-Wide Characterization of QYYZ-Like PRRSV During 2018–2021

Xiang

Tang

et al. 2022

Front. Vet. Sci.

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Analysis of the recombination and evolution of the new type mutant pseudorabies virus XJ5 in China

Jiang,

Cheng,

Pan

et al. 2024

BMC Genomics

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Pseudorabies have caused enormous economic losses in China’s pig industry and have recurred on many large pig farms since late 2011. The disease is caused by highly pathogenic, antigenic variant pseudorabies virus (vPRV) strains. Our laboratory isolated a pseudorabies virus in 2015 and named it XJ5. The pathogenic ability of this mutant strain was much stronger than that of the original isolate. After we sequenced its whole genome (GenBank accession number: OP512542), we found that its overall structure was not greatly changed compared with that of the previous strain Ea (KX423960.1). The whole genome alignment showed that XJ5 had a strong genetic relationship with the strains isolated in China after 2012 reported in GenBank. Based on the isolation time of XJ5 and the mutation and recombination analysis of programs, we found that the whole genome homology of XJ5 and other strains with Chinese isolates was greater than 95%, while the homology with strains outside Asia was less than 94%, which indicated that there may be some recombination and mutation patterns. We found that virulent PRV isolates emerged successively in China in 2011 and formed two different evolutionary clades from foreign isolates. At the same time, this may be due to improper immunization and the presence of wild strains in the field, and recent reports have confirmed that Bartha vaccine strains recombine with wild strains to obtain new pathogenic strains. We performed genetic evolution analysis of XJ5 isolated and sequenced in our laboratory to trace its possible mutations and recombination. We found that XJ5 may be the result of natural mutation of a virus in a branch of mutant strains widely existing in China.

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Complete Genome Sequence of a Novel Subgenotype of Porcine Reproductive and Respiratory Syndrome Virus Strain JX/CH/2016, Isolated in Jiangxi, China

Cited by 2 publications

References 10 publications

Genome-Wide Characterization of QYYZ-Like PRRSV During 2018–2021

Genome-Wide Characterization of QYYZ-Like PRRSV During 2018–2021

Analysis of the recombination and evolution of the new type mutant pseudorabies virus XJ5 in China

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