Complete genome sequence of a new enamovirus from Argentina infecting alfalfa plants showing dwarfism symptoms

Bejerman, Nicolás; Giolitti, Fabián; Trucco, Verónica Milagros; Breuil, Soledad de; Dietzgen, Ralf G.; Lenardon, S. L.

doi:10.1007/s00705-016-2854-3

Cited by 32 publications

(30 citation statements)

References 11 publications

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“…La I media general fue cercana al 51% en Manfredi y superior al 80% en Santiago del Estero. Al ser esta enfermedad sistémica causada por un complejo de virus que necesariamente requieren de un vector para su transmisión (Mena Adriano y Ríos, 2010), la alta I registrada evidenció la susceptibilidad del cultivo al vector responsable de la transmisión de la enfermedad (Bejerman et al, 2011;Trucco et al, 2014., Bejerman et al, 2015, Trucco et al, 2016, Bejerman et al, 2016. Al carácter complejo per se de esta enfermedad, ocasionada por infecciones multivirales, se suma la naturaleza perenne de la alfalfa, que la convierte simultáneamente en hospedante y reservorio de los virus del achaparramiento.…”

Section: Incidencia Y Severidad De La Enfermedad En Ambas Localidadesunclassified

“…En el análisis realizado a todas las plantas de alfalfa que presentaban síntomas de la enfermedad, y que fueron recolectadas en distintas zonas productoras de Argentina, se identificaron cinco virus: el AMV (Trucco, de Breuil, Bejerman, Lenardón y Giolitti, 2014), el Alfalfa dwarf virus (ADV) (Bejerman et al, 2011;Bejerman et al, 2015), el Bean leafroll virus (BLRV) (Trucco et al, 2016), el Alfalfa enamovirus 1 (AEV-1) (Bejerman et al, 2016) y el Alfalfa leaf curl virus (ALCV) (Bejerman et al, 2017).…”

Section: Introductionunclassified

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Evaluación de la virosis del achaparramiento de la alfalfa (Medicago sativa L.) en dos ambientes contrastantes de Argentina

Odorizzi¹,

Cornacchione²,

Arolfo³

et al. 2017

AgriS

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Evaluación de la virosis del achaparramiento de la alfalfa (Medicago sativa L.) en dos ambientes contrastantes de ArgentinaOdorizzi, A. S., Cornacchione, M. V., Arolfo, V., Basigalup, D. H., Mijoevich, M. L. y Balzarini, M. resumenEn los últimos años se vienen observando daños crecientes de una enfermedad viral que afecta la producción y la persistencia del cultivo de alfalfa en Argentina. El objetivo de este trabajo fue comparar la incidencia y severidad de la sintomatología de la enfermedad y explorar las relaciones de su dinámica con respecto a precipitaciones (pp) y temperaturas (T) ambientales en seis cultivares comerciales de alfalfa durante el período 2010-2014. Los cultivares CW1010, WL1058, Traful PV INTA, WL903, Monarca SP INTA y LPS8500 fueron evaluados en los campos experimentales de la Estación Experimental Agropecuaria (EEA) Manfredi del Instituto Nacional de Tecnología Agropecuaria (INTA) y de la EEA Santiago del Estero-INTA. En ambos ensayos se registraron incidencias medias entre 50% y 80%, con importante variabilidad interanual y diferencias entre cultivares. La severidad fue mayor para los cultivares evaluados en Santiago del Estero. Los cultivares WL903 y WL1058 presentaron menor severidad, mientras que LPS8500 y Monarca SP INTA presentaron los mayores valores. De las variables climáticas utilizadas para este análisis, la pp (pp + riego en Santiago del Estero) y la T máxima fueron las de mayor peso para explicar la incidencia de la enfermedad. Con respecto a la severidad, la variable que mayor explica su incremento es la T media. EEA Santiago del Estero-INTA. In both trials, mean incidences between 50% and 80% were recorded, with significant interannual variability and differences among cultivars. The severity was higher for the cultivars evaluated in Santiago del Estero. Cultivars WL903 and WL1058 presented the lowest severity, while LPS8500 and Monarca SP INTA showed the highest values. As for the climatic variables used for this analysis, pp (pp + irrigation in Santiago del Estero) and maximum T were the variables that mostly determined the incidence of the disease. Mean T was the most important variable regarding severity.

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Section: Incidencia Y Severidad De La Enfermedad En Ambas Localidadesunclassified

Section: Introductionunclassified

Evaluación de la virosis del achaparramiento de la alfalfa (Medicago sativa L.) en dos ambientes contrastantes de Argentina

Odorizzi¹,

Cornacchione²,

Arolfo³

et al. 2017

AgriS

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“…More recently, emerging viral diseases of alfalfa have been described with the potential to cause serious yield losses. These include a rhabdovirus, diagnosed in alfalfa plants displaying multiple abnormalities [3]; a new enamovirus from Argentina, alfalfa enamovirus-1 (AEV-1), detected in alfalfa plants showing dwarfism symptoms [4]; an AEV isolate from Sudan, designated AEV-2 [5]; a new species of the family Alphaflexiviridae discovered in alfalfa samples exhibiting chlorosis and stunting [6]; and alfalfa leaf curl virus found in plants displaying leaf curling symptoms [7]. …”

Section: Introductionmentioning

confidence: 99%

Characterization of alfalfa virus F, a new member of the genus Marafivirus

et al. 2018

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Viral infections of alfalfa are widespread in major cultivation areas and their impact on alfalfa production may be underestimated. A new viral species, provisionally named alfalfa virus F (AVF), was identified using a virion-associated nucleic acid (VANA) metagenomics-based approach in alfalfa (Medicago sativa L.) samples collected in Southern France. The nucleotide sequence of the viral genome was determined by de-novo assembly of VANA reads and by 5’/3’ RACE with viral RNA extracted from enriched viral particles or with total RNA, respectively. The virus shares the greatest degree of overall sequence identity (~78%) with Medicago sativa marafivirus 1 (MsMV1) recently deduced from alfalfa transcriptomic data. The tentative nucleotide sequence of the AVF coat protein shares ~83% identity with the corresponding region of MsMV1. A sequence search of the predicted single large ORF encoding a polyprotein of 235kDa in the Pfam database resulted in identification of five domains, characteristic of the genus Marafivirus, family Tymoviridae. The AVF genome also contains a conserved “marafibox”, a 16-nt consensus sequence present in all known marafiviruses. Phylogenetic analysis of the complete nucleotide sequences of AVF and other viruses of the family Tymoviridae grouped AVF in the same cluster with MsMV1. In addition to 5’ and 3’ terminal extensions, the identity of the virus was confirmed by RT-PCRs with primers derived from VANA-contigs, transmission electron microscopy with virus-infected tissues and transient expression of the viral coat protein gene using a heterologous virus-based vector. Based on the criteria demarcating species in the genus Marafivirus that include overall sequence identity less than 80% and coat protein identity less than 90%, we propose that AVF represents a distinct viral species in the genus Marafivirus, family Tymoviridae.

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“…Alfalfa may serve as a natural reservoir for dissemination of viruses to other agriculturally important crops, although its exact role in the epidemiology of viruses in other crops is not well documented [5,6]. In recent years, emerging viral diseases of alfalfa with the capacity to cause more serious yield losses, have been described [7,8]. In addition, synergetic interactions of multiple viral infections can threaten alfalfa production [7,9].…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, emerging viral diseases of alfalfa with the capacity to cause more serious yield losses, have been described [7,8]. In addition, synergetic interactions of multiple viral infections can threaten alfalfa production [7,9]. …”

Section: Introductionmentioning

confidence: 99%

Alfalfa virus S, a new species in the family Alphaflexiviridae

Nemchinov

Grinstead²,

Mollov³

2017

PLoS ONE

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A new species of the family Alphaflexiviridae provisionally named alfalfa virus S (AVS) was discovered in alfalfa samples originating from Sudan. A complete nucleotide sequence of the viral genome consisting of 8,349 nucleotides excluding the 3’ poly(A) tail was determined by high throughput sequencing (HTS) on an Illumina platform. NCBI BLAST searches revealed that the virus shares the greatest degree of sequence identity with members of the family Alphaflexiviridae, genus Allexivirus. The AVS genome contains six computationally-predicted open reading frames (ORF) encoding viral replication protein, triple gene block protein 1 (TGB1), TGB2, TGB3-like protein, unknown 38.4 kDa protein resembling serine-rich 40 kDa protein characteristic for allexiviruses, and coat protein (CP). AVS lacks a clear 3’ proximal ORF that encodes a nucleic acid-binding protein typical for allexiviruses. The identity of the virus was confirmed by RT-PCR with primers derived from the HTS-generated sequence, dot blot hybridization with DIG-labeled virus-specific RNA probes, and Western blot analysis with antibodies produced against a peptide derived from the CP sequence. Transmission electron microscopic observations of the infected tissues showed the presence of filamentous particles similar to allexiviruses in their length and appearance. To the best of our knowledge, this is the first report on the identification of a putative allexivirus in alfalfa (Medicago sativa). The genome sequence of AVS has been deposited in NCBI GenBank on 03/02/2016 as accession № KY696659.

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Complete genome sequence of a new enamovirus from Argentina infecting alfalfa plants showing dwarfism symptoms

Cited by 32 publications

References 11 publications

Evaluación de la virosis del achaparramiento de la alfalfa (Medicago sativa L.) en dos ambientes contrastantes de Argentina

Evaluación de la virosis del achaparramiento de la alfalfa (Medicago sativa L.) en dos ambientes contrastantes de Argentina

Characterization of alfalfa virus F, a new member of the genus Marafivirus

Alfalfa virus S, a new species in the family Alphaflexiviridae

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