Complete genome sequence of a novel sea otterpox virus

Abstract

Members of the Poxviridae family are large, double-stranded DNA viruses that replicate in the cytoplasm of their host cells. The subfamily Chordopoxvirinae contains viruses that infect a wide range of vertebrates including marine mammals within the Balaenidae, Delphinidae, Mustelidae, Odobenidae, Otariidae, Phocidae, and Phocoenidae families. Recently, a novel poxvirus was found in a northern sea otter pup (Enhydra lutris kenyoni) that stranded in Alaska in 2009. The phylogenetic relationships of marine mammal poxviruses are not well established because of the lack of complete genome sequences. The current study sequenced the entire sea otterpox virus Enhydra lutris kenyoni (SOPV-ELK) genome using an Illumina MiSeq sequencer. The SOPV-ELK genome is the smallest poxvirus genome known at 127,879 bp, is 68.7% A+T content, is predicted to encode 132 proteins, and has 2546 bp inverted terminal repeats at each end. Genetic and phylogenetic analyses based on the concatenated amino acid sequences of 7 chorodopoxvirus core genes revealed the SOPV-ELK is 52.5-74.1% divergent from other known chordopoxviruses and is most similar to pteropoxvirus from Australia (PTPV-Aus). SOPV-ELK represents a new chordopoxvirus species and may belong to a novel genus. SOPV-ELK encodes eight unique genes. While the function of six predicted genes remains unknown, two genes appear to function as novel immune-modulators. SOPV-ELK-003 appears to encode a novel interleukin-18 binding protein (IL-18 BP), based on limited sequence and structural similarity to other poxviral IL-18 BPs. SOPV-ELK-035 appears to encode a novel tumor necrosis factor receptor-like (TNFR) protein that may be associated with the depression of the host's antiviral response. Additionally, SOPV-ELK-036 encodes a tumor necrosis factor-like apoptosis-inducing ligand (TRAIL) protein that has previously only been found in PTPV-Aus. The SOPV-ELK genome is the first mustelid poxvirus and only the second poxvirus from a marine mammal to be fully sequenced. Sequencing of the SOPV-ELK genome is an important step in unraveling the position of marine mammal poxviruses within the larger Poxviridae phylogenetic tree and provides the necessary sequence to develop molecular tools for future diagnostics and epidemiological studies.

Viral and metazoan poxins are cGAMP-specific nucleases that restrict cGAS–STING signalling

“…The metazoan poxin sequences from moth and butterfly genomes ( Lepidoptera , bottom) share homology throughout the entire poxin protein and exhibit identical 2′3′ cGAMP degradation activity, but the alignment with viral poxins does not allow definitive assignment of the catalytic residues. Phylogram schematics are based on previous analyses 52–55 . b , Coomasie-stained SDS-PAGE analysis of recombinant SUMO2-tagged poxin homologue proteins.…”

Faculty Opinions recommendation of Viral and metazoan poxins are cGAMP-specific nucleases that restrict cGAS-STING signalling.

“…The metazoan poxin sequences from moth and butterfly genomes (Lepidoptera, bottom) share homology throughout the entire poxin protein and exhibit identical 2′3′ cGAMP degradation activity, but the alignment with viral poxins does not allow definitive assignment of the catalytic residues. Phylogram schematics are based on previous analyses [52][53][54][55] . b, Coomasie-stained SDS-PAGE analysis of recombinant SUMO2tagged poxin homologue proteins.…”

Parapoxvirus species revisited by whole genome sequencing: A retrospective analysis of bovine virus isolates

“…The PPV species constitute a genus among the subfamily Chordopoxvirinae of the family Poxviridae . Poxviral DNA genomes can vary in size from 128 (seal PPV) to 360 (Canarypox virus) kilobases (kb) and contain inverted terminal repeats (ITRs) of up to 13 kb ( Asif et al, 2021 ; Jacob et al, 2018 ). PPV can easily be delineated from Orthopoxviruses by their unique virion architecture (regular crisscross tubular core structure) and the high G + C content (64 %) of the genome ( Rziha and Büttner, 2021 ).…”