Complete Genome Sequence of a Novel Polyhydroxyalkanoate (PHA) Producer, Jeongeupia sp. USM3 (JCM 19920) and Characterization of Its PHA Synthases

Zain, Noor-Afiqah Ahmad; Ng, Lee-Mei; Foong, Choon Pin; Tai, Yen Teng; Nanthini, Jayaram; Sudesh, Kumar

doi:10.1007/s00284-019-01852-z

Cited by 11 publications

(3 citation statements)

References 42 publications

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“…The genes responsible for PHA biosynthesis usually clustered together in the bacterial genome ( Rehm and Steinbüchel, 1999 ), such as the most well-known genetic organization of phaCAB operon in Ralstonia eutropha H16 ( Peoples and Sinskey, 1989 ), the phaCA operon in Jeongeupia sp. USM3 ( Zain et al, 2020 ), the phaEC operon in Neptunomonas concharum ( Pu et al, 2020b ). Interestingly, the presence of phasin gene in a PHA operon is rarely reported.…”

Section: Resultsmentioning

confidence: 99%

Engineering Vibrio alginolyticus as a novel chassis for PHB production from starch

Tian²,

Lian³

et al. 2023

Front. Bioeng. Biotechnol.

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Vibrio alginolyticus LHF01 was engineered to efficiently produce poly-3-hydroxybutyrate (PHB) from starch in this study. Firstly, the ability of Vibrio alginolyticus LHF01 to directly accumulate PHB using soluble starch as the carbon source was explored, and the highest PHB titer of 2.06 g/L was obtained in 18 h shake flask cultivation. Then, with the analysis of genomic information of V. alginolyticus LHF01, the PHB synthesis operon and amylase genes were identified. Subsequently, the effects of overexpressing PHB synthesis operon and amylase on PHB production were studied. Especially, with the co-expression of PHB synthesis operon and amylase, the starch consumption rate was improved and the PHB titer was more than doubled. The addition of 20 g/L insoluble corn starch could be exhausted in 6-7 h cultivation, and the PHB titer was 4.32 g/L. To the best of our knowledge, V. alginolyticus was firstly engineered to produce PHB with the direct utilization of starch, and this stain can be considered as a novel host to produce PHB using starch as the raw material.

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Section: Resultsmentioning

confidence: 99%

Engineering Vibrio alginolyticus as a novel chassis for PHB production from starch

Tian²,

Lian³

et al. 2023

Front. Bioeng. Biotechnol.

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“…PHA is one of the PNPs generated spontaneously in the bacterial cytoplasm. PHA belongs to the aliphatic polyesters family of biodegradable and biocompatible polymers [ 304 , 305 , 306 ]. PHA is produced spontaneously by some bacteria in the form of nanosized granules under unbalanced growth conditions, such as an excess of carbon source and nutritional limitations, such as nitrogen, oxygen, and phosphorus [ 307 , 308 , 309 ].…”

Section: Biologically Synthesized Biodegradable Polyhydroxyalkanoate-...mentioning

confidence: 99%

Exploring Various Techniques for the Chemical and Biological Synthesis of Polymeric Nanoparticles

et al. 2022

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Nanoparticles (NPs) have remarkable properties for delivering therapeutic drugs to the body’s targeted cells. NPs have shown to be significantly more efficient as drug delivery carriers than micron-sized particles, which are quickly eliminated by the immune system. Biopolymer-based polymeric nanoparticles (PNPs) are colloidal systems composed of either natural or synthetic polymers and can be synthesized by the direct polymerization of monomers (e.g., emulsion polymerization, surfactant-free emulsion polymerization, mini-emulsion polymerization, micro-emulsion polymerization, and microbial polymerization) or by the dispersion of preformed polymers (e.g., nanoprecipitation, emulsification solvent evaporation, emulsification solvent diffusion, and salting-out). The desired characteristics of NPs and their target applications are determining factors in the choice of method used for their production. This review article aims to shed light on the different methods employed for the production of PNPs and to discuss the effect of experimental parameters on the physicochemical properties of PNPs. Thus, this review highlights specific properties of PNPs that can be tailored to be employed as drug carriers, especially in hospitals for point-of-care diagnostics for targeted therapies.

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“…Hence, PhaC has a dominant function in determining the general properties of produced PHA, such as molecular weight, monomer composition, and polydispersity. Many studies were carried out to characterize PhaCs from various environments [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

In Vivo Characterization and Application of the PHA Synthase from Azotobacter vinelandii for the Biosynthesis of Polyhydroxyalkanoate Containing 4-Hydroxybutyrate

et al. 2021

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Polyhydroxyalkanoate (PHA) is a biodegradable thermoplastic naturally synthesized by many microorganisms, and the PHA synthase (PhaC) is known to be the key enzyme involved in determining the material properties and monomer composition of the produced PHA. The ability to exploit widely distributed, commonly found soil microorganisms such as Azotobacter vinelandii to synthesize PHA containing the lipase-degradable 4-hydroxybutyrate (4HB) monomer will allow for convenient production of biocompatible and flexible PHA. Comparisons between the A. vinelandii wild type and mutant strains, with and without a surface layer (S-layer), respectively, in terms of gene or amino acid sequences, synthase activity, granule morphology, and PHA productivity, revealed that the S-layer is the sole factor affecting PHA biosynthesis by A. vinelandii. Based on PHA biosynthesis using different carbon sources, the PhaC of A. vinelandii showed specificity for short-chain-length PHA monomers, making it a member of the Class I PHA synthases. In addition, it was proven that the PhaC of A. vinelandii has the inherent ability to polymerize 4-hydroxybutyrate (4HB) and the mediated accumulation of PHA with 4HB fractions ranging from 10 mol% to as high as 22 mol%. The synthesis of biocompatible PHA containing tailorable amounts of 4HB with an expanded range of elasticity and lipase-degradability will enable a wider range of applications in the biomedical field.

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Complete Genome Sequence of a Novel Polyhydroxyalkanoate (PHA) Producer, Jeongeupia sp. USM3 (JCM 19920) and Characterization of Its PHA Synthases

Cited by 11 publications

References 42 publications

Engineering Vibrio alginolyticus as a novel chassis for PHB production from starch

Engineering Vibrio alginolyticus as a novel chassis for PHB production from starch

Exploring Various Techniques for the Chemical and Biological Synthesis of Polymeric Nanoparticles

In Vivo Characterization and Application of the PHA Synthase from Azotobacter vinelandii for the Biosynthesis of Polyhydroxyalkanoate Containing 4-Hydroxybutyrate

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