Complete Genome Sequence of a Novel Human Gammapapillomavirus Isolated from a Cervical Swab in Luxembourg

Latsuzbaia, Ardashel; Arbyn, Marc; Dutta, Sankhadeep; Fischer, Marc; Gheit, Tarik; Tapp, Jessica; Tommasino, Massimo; Weyers, Steven; Mossong, Joël

doi:10.1128/genomea.00114-18

Cited by 10 publications

(10 citation statements)

References 8 publications

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“…Interestingly, none of them were identified in a variety of 653 cutaneous samples with the same testing approach [21]. These results, together with previously published findings [13,17,26,28,[40][41][42][43], suggest the possible adaptation of the mentioned HPV types to the mucosal epithelium [44].…”

Section: Discussionsupporting

confidence: 56%

Assessing Gammapapillomavirus infections of mucosal epithelia with two broad-spectrum PCR protocols

et al. 2020

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Background: Human papillomaviruses (HPVs) have been divided into mucosal and cutaneous types according to their primary epithelial tissue tropism. However, recent studies showed the presence of several cutaneous types in mucosal lesions and healthy mucosa from different anatomical sites. Methods: Here, the HPV prevalence and type-specific distribution were assessed in a variety of mucosal samples from 435 individuals using a combination of two established broad-spectrum primer systems: Gamma-PV PCR and CUT PCR.Results: Overall HPV prevalence in anal canal swabs, cervical cancer biopsies, genital warts and oral swabs was 85, 47, 62 and 4%, respectively. In anal canal swabs, Alpha-PVs were most frequently found (59%), followed by Gamma-(37%) and Beta-PVs (4%). The prevalence and persistence of HPV infection in the anal canal of 226 individuals were further explored. Overall HPV, Gamma-PVs and multiple HPV infections were significantly higher in men vs. women (p = 0.034, p = 0.027 and p = 0.003, respectively); multiple HPV infections were more common in individuals ≤40 years (p = 0.05), and significantly higher prevalence of Gamma-PVs and multiple HPV infections was observed in HIV-1-positive vs. HIV-1-negative individuals (p = 0.003 and p = 0.04, respectively). Out of 21 patients with follow-up anal swabs, only one persistent infection with the same type (HPV58) was detected. Conclusions: Our findings suggest that Gamma-PVs (except species Gamma-6) are ubiquitous viruses with dual muco-cutaneous tissue tropism. Anal canal Gamma-PV infections may be associated with sexual behavior and the host immune status. This study expands the knowledge on Gamma-PVs' tissue tropism, providing valuable data on the characteristics of HPV infection in the anal canal.

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Section: Discussionsupporting

confidence: 56%

Assessing Gammapapillomavirus infections of mucosal epithelia with two broad-spectrum PCR protocols

et al. 2020

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“…While PCR-based HPV assays detect by design only a limited number of targeted genotypes, next generation sequencing (NGS) may be an alternative approach for universal HPV detection and characterization [ 16 , 21 , 22 , 23 , 24 , 25 ]. This technology facilitated the detection of novel HPV genotypes and variants [ 14 , 22 , 26 ] resulting in the identification of 222 HPV types and more than 100 lineages/sublineages [ 27 , 28 ]. Rolling-circle amplification (RCA) has been developed for the random amplification of circular DNA genomes, including viruses from the Papillomaviridae family [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

“…It was first used for HPV amplification and sequencing by Rector et al before the availability of NGS [ 30 ], and since then few studies have used this approach for investigating HPV [ 14 , 31 , 32 ]. The RCA technology allows unbiased random amplification of HPV genome without prior knowledge of the sequence and therefore has a great potential for improving characterization of both known and novel HPV genotypes [ 26 , 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization and Diversity of 243 Complete Human Papillomavirus Genomes in Cervical Swabs Using Next Generation Sequencing

Latsuzbaia

Wienecke-Baldacchino

Tapp

et al. 2020

Viruses

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In recent years, next generation sequencing (NGS) technology has been widely used for the discovery of novel human papillomavirus (HPV) genotypes, variant characterization and genotyping. Here, we compared the analytical performance of NGS with a commercial PCR-based assay (Anyplex II HPV28) in cervical samples of 744 women. Overall, HPV positivity was 50.2% by the Anyplex and 45.5% by the NGS. With the NGS, we detected 25 genotypes covered by Anyplex and 41 additional genotypes. Agreement between the two methods for HPV positivity was 80.8% (kappa = 0.616) and 84.8% (kappa = 0.652) for 28 HPV genotypes and 14 high-risk genotypes, respectively. We recovered and characterized 243 complete HPV genomes from 153 samples spanning 40 different genotypes. According to phylogenetic analysis and pairwise distance, we identified novel lineages and sublineages of four high-risk and 16 low-risk genotypes. In total, 17 novel lineages and 14 novel sublineages were proposed, including novel lineages of HPV45, HPV52, HPV66 and a novel sublineage of HPV59. Our study provides important genomic insights on HPV types and lineages, where few complete genomes were publicly available.

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“…Wirusy brodawczaka ludzkiego (human papilloma virus -HPV) należą do rodziny Papillomaviridae i rodzaju Papillomavirus. W Międzynarodowym Ośrodku Referencyjnym Wirusa Brodawczaka Ludzkiego (International Human Papillomavirus Reference Center) zsekwencjonowano i nazwano łącznie 226 typów tego wirusa [1]. HPV jest wirusem pozbawionym otoczki, składającym się z kapsydu (zbudowanego z 72 kapsomerów) zawierającego pojedynczą cząsteczkę dwuniciowego kolistego DNA.…”

Section: Wprowadzenieunclassified

“…Największa jest grupa α obejmująca typy HPV o wysokim ryzyku onko-introDUction Human papillomaviruses (HPV) belong to the family Papillomaviridae and genus Papillomavirus. In the International Human Papillomavirus Reference Center, 226 types of this virus have been sequenced and assigned a name [1]. HPV is a non-enveloped virus, formed of a capsid (built up of 72 capsomers) containing a single, circular double-stranded DNA molecule.…”

Section: Wprowadzeniementioning

confidence: 99%

Human papillomavirus (HPV) coinfection with other sexually transmitted infections in patients of the Department of Dermatology and Venereology at the Medical University of Warsaw

Bukowska¹,

Młynarczyk-Bonikowska²,

Malejczyk³

et al. 2020

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Introduction. Infections with the genital types of human papillomavirus (HPV) are usually eliminated by the immune system within 6-18 months. Other concurrent sexually transmitted infections may be one of the factors contributing to the infection and its longer persistence. Objective. To assess the prevalence of HPV and coinfections with other sexually transmitted infections. Material and methods. Forty women with at least one sexually transmitted infection and 45 women from the control group were examined. For gonorrhea and chlamydial infection diagnostics, culture and direct immunofluorescence were applied, respectively. In parallel, both infections were diagnosed using real-time PCR. Syphilis was diagnosed using serological tests VDRL and FTA-ABS tests, and HIV infection with enzymatic immunoassay. Real-time PCR was used to detect HPV6, 11, 16 and 18. Results. HPV infection was found in 60% of women in the study group and 42.22% in the control group. HPV16 infection was diagnosed in 58.3% of women infected with C. trachomatis, 57.9% with gonorrhea, 16.7% with syphilis and 26.7% of women in the control group. The percentage of those infected with two types of HPV in the study group was 22.5%, and 13.33% in the control group. A relationship was found between HPV16 infection and gonorrhea and chlamydial infection and between gonorrhea and infection with two different types of HPV. Conclusions. The obtained results indicate the influence of C. trachomatis and N. gonorrhoeae infections on the persistence of oncogenic HPV infections, which may lead to an increased risk of developing pre-cancerous lesions.

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Complete Genome Sequence of a Novel Human Gammapapillomavirus Isolated from a Cervical Swab in Luxembourg

Cited by 10 publications

References 8 publications

Assessing Gammapapillomavirus infections of mucosal epithelia with two broad-spectrum PCR protocols

Assessing Gammapapillomavirus infections of mucosal epithelia with two broad-spectrum PCR protocols

Characterization and Diversity of 243 Complete Human Papillomavirus Genomes in Cervical Swabs Using Next Generation Sequencing

Human papillomavirus (HPV) coinfection with other sexually transmitted infections in patients of the Department of Dermatology and Venereology at the Medical University of Warsaw

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