Complete genome sequence of a novel bovine hepacivirus from Yunnan, China

Xin, Qiang; Shen, Xuehui; Peng, Honghong; Guo, Xin; He, Zhihai; Yao, Mingguo; Fu, Guozhang; Cui, Yu-Jun; Zhang, Xianglilan; Huang, Yong; Fan, Hang; Du, Chunhong; Tong, Yigang; Mi, Zhiqiang

doi:10.1007/s00705-020-04611-9

Cited by 7 publications

(19 citation statements)

References 21 publications

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“…This could have resulted from frequent international trade of live cattle, which can facilitate transboundary transmission of BovHepV. In addition, BovHepV has only been detected in cattle from limited areas of China, i.e., Guangdong, Jiangsu, Yunnan, and Sichuan provinces [28][29][30][31]. Epidemiological surveys in broader areas may better reveal the genetic diversity and epidemiological characteristics of BovHepV circulating in China.…”

Section: Discussionmentioning

confidence: 99%

“…BovHepV is hepatotropic, and causes acute or persistent infections in cattle [10,23]. Since its first discovery in cattle from Germany [10] and Ghana [11] in 2015, it has been detected in Brazil [24,25], Turkey [26], USA [27], China [28][29][30][31], and Italy [32], suggesting a worldwide distribution of BovHepV. As per the demarcation criteria for HCV genotyping and subtyping, BovHepV strains have been classified into two genotypes, and genotype 1 could be further divided into seven subtypes (A to G) [28,31].…”

Section: Introductionmentioning

confidence: 99%

“…Since its first discovery in cattle from Germany [10] and Ghana [11] in 2015, it has been detected in Brazil [24,25], Turkey [26], USA [27], China [28][29][30][31], and Italy [32], suggesting a worldwide distribution of BovHepV. As per the demarcation criteria for HCV genotyping and subtyping, BovHepV strains have been classified into two genotypes, and genotype 1 could be further divided into seven subtypes (A to G) [28,31]. In China, BovHepV has been detected in cattle in Guangdong, Jiangsu, Yunnan, and Sichuan provinces, with the positive rate of viral RNA ranging from 2.78% to 7.84% [28][29][30][31].…”

Section: Introductionmentioning

confidence: 99%

“…As per the demarcation criteria for HCV genotyping and subtyping, BovHepV strains have been classified into two genotypes, and genotype 1 could be further divided into seven subtypes (A to G) [28,31]. In China, BovHepV has been detected in cattle in Guangdong, Jiangsu, Yunnan, and Sichuan provinces, with the positive rate of viral RNA ranging from 2.78% to 7.84% [28][29][30][31]. All of these BovHepV strains identified in China belong to genotype 1, with the exception of a strain in the genotype 2 [31].…”

Section: Introductionmentioning

confidence: 99%

“…In China, BovHepV has been detected in cattle in Guangdong, Jiangsu, Yunnan, and Sichuan provinces, with the positive rate of viral RNA ranging from 2.78% to 7.84% [28][29][30][31]. All of these BovHepV strains identified in China belong to genotype 1, with the exception of a strain in the genotype 2 [31].…”

Section: Introductionmentioning

confidence: 99%

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A Novel Subtype of Bovine Hepacivirus Identified in Ticks Reveals the Genetic Diversity and Evolution of Bovine Hepacivirus

Shao

Guo

et al. 2021

Viruses

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Hepaciviruses represent a group of viruses that pose a significant threat to the health of humans and animals. New members of the genus Hepacivirus in the family Flaviviridae have recently been identified in a wide variety of host species worldwide. Similar to the Hepatitis C virus (HCV), bovine hepacivirus (BovHepV) is hepatotropic and causes acute or persistent infections in cattle. BovHepVs are distributed worldwide and classified into two genotypes with seven subtypes in genotype 1. In this study, three BovHepV strains were identified in the samples of ticks sucking blood on cattle in the Guangdong province of China, through unbiased high-throughput sequencing. Genetic analysis revealed the polyprotein-coding gene of these viral sequences herein shared 67.7–84.8% nt identity and 76.1–95.6% aa identity with other BovHepVs identified worldwide. As per the demarcation criteria adopted for the genotyping and subtyping of HCV, these three BovHepV strains belonged to a novel subtype within the genotype 1. Additionally, purifying selection was the dominant evolutionary pressure acting on the genomes of BovHepV, and genetic recombination was not common among BovHepVs. These results expand the knowledge about the genetic diversity and evolution of BovHepV distributed globally, and also indicate genetically divergent BovHepV strains were co-circulating in cattle populations in China.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A Novel Subtype of Bovine Hepacivirus Identified in Ticks Reveals the Genetic Diversity and Evolution of Bovine Hepacivirus

Shao

Guo

et al. 2021

Viruses

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Two bovine hepacivirus genome sequences from U.S. cattle

Workman,

Harhay,

Groves

et al. 2024

J VET Diagn Invest

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Bovine hepacivirus (BoHV) is closely related to the hepatitis C virus (HCV) in humans and can cause both acute and chronic liver infections in cattle. BoHV was first identified in Ghana and Germany in 2015 and since then it has been detected and characterized in other countries around the world, but no strains have been sequenced from U.S. cattle. To date, BoHV has been classified into 2 genotypes (1 and 2), with genotype 1 being further divided into 11 subtypes (A–K). However, the true genetic diversity of BoHV is likely underestimated given limited surveillance and a lack of published genome sequences. Here, we sequenced 2 nearly complete BoHV genomes from serum samples collected in 2019 from beef cattle in Missouri. Sequence comparisons and phylogenetic analysis showed that isolate MARC/2019/60 had high sequence homology with genotype 1, subtype E isolates from China. In contrast, isolate MARC/2019/50 represented a novel BoHV subtype within genotype 2. Thus, we report the first genomic characterization of BoHV isolates from U.S. cattle, and the second complete BoHV2 genome worldwide. This work increases our knowledge of the global genetic diversity of BoHV and demonstrates the co-circulation of divergent BoHV strains in U.S. cattle.

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Genome Characterization and Phylogenetic Analysis of Bovine Hepacivirus in Inner Mongolia, Northeastern China

Liu

Guo

et al. 2022

Zoonoses

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Objective: Bovine hepacivirus (BovHepV) is a new member of the genus Hepacivirus in the family Flaviviridae, which has been detected in cattle in more than seven countries. The purpose of this study was to identify and genetically characterize BovHepV in cattle in Inner Mongolia, northeastern (NE) China. Methods: A total of 116 serum samples from cattle were collected from HulunBuir in Inner Mongolia from April to May, 2021, and were divided into three pools for metagenomic sequencing. The samples were verified with semi-nested RT-PCR with primers based on the BovHepV sequences obtained from metagenomic sequencing. The complete genomes of BovHepV were amplified, and were used for genome characterization and phylogenetic analysis. Results: BovHepV was detected in two pools through metagenomic sequencing. Five BovHepV positive samples were identified in Yakeshi of HulunBuir, thus indicating a prevalence of 8.8% (5/57). Two 8840 nucleotide long BovHepV strains YKS01/02 were amplified from the positive samples and showed 79.3%–91.9% nucleotide sequence identity with the discovered BovHepV strains. Phylogenetic analysis classified the YKS01/02 strains into BovHepV subtype G group. Conclusion: This study reports the first identification of BovHepV in cattle in northeastern China, and expands the known geographical distribution and genetic diversity of BovHepV in the country.

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Complete genome sequence of a novel bovine hepacivirus from Yunnan, China

Cited by 7 publications

References 21 publications

A Novel Subtype of Bovine Hepacivirus Identified in Ticks Reveals the Genetic Diversity and Evolution of Bovine Hepacivirus

A Novel Subtype of Bovine Hepacivirus Identified in Ticks Reveals the Genetic Diversity and Evolution of Bovine Hepacivirus

Two bovine hepacivirus genome sequences from U.S. cattle

Genome Characterization and Phylogenetic Analysis of Bovine Hepacivirus in Inner Mongolia, Northeastern China

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