Complete Genome Sequence of “
            <i>Candidatus</i>
            Phytoplasma asteris” QS2022, a Plant Pathogen Associated with Lettuce Chlorotic Leaf Rot Disease in China

Yan, Xiaohua; Lin, Jixiu; Liu, Yanzhi; Huang, P; Liu, Jianmi; Hu, Qibin; Li, Yalu; Pei, Shen-Chian; Huang, Weijie; Kuo, Chih-Horng

doi:10.1128/mra.00306-23

Cited by 2 publications

(3 citation statements)

References 15 publications

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“…Moreover, the ANI method has been suggested as shifting the gold standard for the classification of prokaryotic species, especially in the era of big data, with an extreme increase in complete prokaryotic genome sequences [47,[75][76][77][78]. In contrast to the three strains MDGZ-01, which infect mulberry (Morus alba), Zhengzhou [21], which is associated with paulownia (Paulownia fortunei), and QS2022 [18], which has been reported in lettuce (Lactuca sativa) in China, the M8 and M33 strains originated from the same field and host plant. These results are consistent with the occurrence of different 16SrI subgroups on individual fields during the season.…”

Section: Discussionmentioning

confidence: 99%

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Divergence within the Taxon ‘Candidatus Phytoplasma asteris’ Confirmed by Comparative Genome Analysis of Carrot Strains

Toth,

Ilic,

Huettel

et al. 2024

Microorganisms

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Phytoplasmas are linked to diseases in hundreds of economically important crops, including carrots. In carrots, phytoplasmosis is associated with leaf chlorosis and necrosis, coupled with inhibited root system development, ultimately leading to significant economic losses. During a field study conducted in Baden-Württemberg (Germany), two strains of the provisional taxon ‘Candidatus Phytoplasma asteris’ were identified within a carrot plot. For further analysis, strains M8 and M33 underwent shotgun sequencing, utilising single-molecule-real-time (SMRT) long-read sequencing and sequencing-by-synthesis (SBS) paired-end short-read sequencing techniques. Hybrid assemblies resulted in complete de novo assemblies of two genomes harboring circular chromosomes and two plasmids. Analyses, including average nucleotide identity and sequence comparisons of established marker genes, confirmed the phylogenetic divergence of ‘Ca. P. asteris’ and a different assignment of strains to the 16S rRNA subgroup I-A for M33 and I-B for M8. These groups exhibited unique features, encompassing virulence factors and genes, associated with the mobilome. In contrast, pan-genome analysis revealed a highly conserved gene set related to metabolism across these strains. This analysis of the Aster Yellows (AY) group reaffirms the perception of phytoplasmas as bacteria that have undergone extensive genome reduction during their co-evolution with the host and an increase of genome size by mobilome.

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Section: Discussionmentioning

confidence: 99%

“…Among the analysed genomes are 'Ca. P. asteris' strains infecting various hosts, including onion, lettuce [17,18], maize [19], grapevine (acc. no.…”

Section: Introductionmentioning

confidence: 99%

Divergence within the Taxon ‘Candidatus Phytoplasma asteris’ Confirmed by Comparative Genome Analysis of Carrot Strains

Toth,

Ilic,

Huettel

et al. 2024

Microorganisms

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“…gov/ datas ets/ taxon omy/ 33926/ ). In this study, we summarize the information on the 27 complete genomes, including 16SrI (Bai et al, 2006;Cao et al, 2021;Coetzee et al, 2019;Luo et al, 2022;Orlovskis et al, 2017;Oshima et al, 2004;Yan et al, 2023), II (Chang et al, 2015;Kirdat et al, 2023b;Li et al, 2023;Rodrigues Jardim et al, 2022;Tan et al, 2021), III (unpublished), V (Debonneville et al, 2022;Duckeck et al, 2023;, VIII (Huang et al, 2022), X (Kube et al, 2008), XI (Zhang et al, 2023), XII (Andersen et al, 2013;Tran-Nguyen et al, 2008) and XIV (unpublished). Eight complete genomes were obtained for the 16SrI group.…”

Section: G Enomic Information Of Phy Topl a S Ma Smentioning

confidence: 99%

Phytoplasma: A plant pathogen that cannot be ignored in agricultural production—Research progress and outlook

Wang,

Bai,

et al. 2024

Molecular Plant Pathology

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Phytoplasmas are phloem‐restricted plant‐pathogenic bacteria transmitted by insects. They cause diseases in a wide range of host plants, resulting in significant economic and ecological losses worldwide. Research on phytoplasmas has a long history, with significant progress being made in the past 30 years. Notably, with the rapid development of phytoplasma research, scientists have identified the primary agents involved in phytoplasma transmission, established classification and detection systems for phytoplasmas, and 243 genomes have been sequenced and assembled completely or to draft quality. Multiple possible phytoplasma effectors have been investigated, elucidating the molecular mechanisms by which phytoplasmas manipulate their hosts. This review summarizes recent advances in phytoplasma research, including identification techniques, host range studies, whole‐ or draft‐genome sequencing, effector pathogenesis and disease control methods. Additionally, future research directions in the field of phytoplasma research are discussed.

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Complete Genome Sequence of “ Candidatus Phytoplasma asteris” QS2022, a Plant Pathogen Associated with Lettuce Chlorotic Leaf Rot Disease in China

Abstract: The complete genome sequence of “ Candidatus Phytoplasma asteris” QS2022, which consists of one 834,303-bp circular chromosome, is presented in this work. This bacterium is associated with lettuce chlorotic leaf rot disease in Fujian Province, China.

Cited by 2 publications

References 15 publications

Divergence within the Taxon ‘Candidatus Phytoplasma asteris’ Confirmed by Comparative Genome Analysis of Carrot Strains

Divergence within the Taxon ‘Candidatus Phytoplasma asteris’ Confirmed by Comparative Genome Analysis of Carrot Strains

Phytoplasma: A plant pathogen that cannot be ignored in agricultural production—Research progress and outlook

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