Complete genome sequence and pathogenicity of fowl adenovirus serotype 4 involved in hydropericardium syndrome in Southwest China

Guan, Ru; Tian, Yiming; Han, Xing; Yang, Xin; Wang, Hongning

doi:10.1016/j.micpath.2018.02.012

Cited by 30 publications

(15 citation statements)

References 42 publications

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“…In 2015, an infectious outbreak spread rapidly amongst broiler chickens in China and caused high rates of mortality. Pericardial effusions and liver lesions were the main clinical features of this disease [4, 5], and our research revealed that FAdV was the pathogen for the outbreak [6]. At present, the results of epidemiological surveys indicated that FAdV serotype 4 (FAdV-4) was the main serotype that caused this avian adenoviral epidemic.…”

Section: Introductionmentioning

confidence: 91%

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LAMP real-time turbidity detection for fowl adenovirus

et al. 2019

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Background Fowl adenovirus (FAdV) is an infectious agent that can cause jaundice, severe anaemia, dyspnoea and reproductive disorders in fowls. Since 2015, FAdV disease has been rapidly spreading among broiler chickens in China, where it has caused significant economic losses. In this study, a loop-mediated isothermal amplification (LAMP) real-time turbidity method with strong specificity to FAdV was established. Results The established assay was specific to FAdV-4, and the lower limit of detection was 75 copies/μL of extracted DNA. The positive detection rate for the suspected tissue samples was 33.3% (14/42), which was consistent with that of the real-time PCR. Conclusion The proposed LAMP method can quickly and accurately detect prevalent FAdV via real-time turbidity assay, thereby providing a diagnostic platform for the prevention and control of the FAdV disease.

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Section: Introductionmentioning

confidence: 91%

“…Fowl adenovirus (FAdV) is a member of the genus Aviadenovirus [1]. In the last 20 years, different serotypes of FAdVs have been distributed worldwide [2–4]. In 2015, an infectious outbreak spread rapidly amongst broiler chickens in China and caused high rates of mortality.…”

Section: Introductionmentioning

confidence: 99%

LAMP real-time turbidity detection for fowl adenovirus

et al. 2019

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“…FAdV strains sequenced in this study were either isolated from documented clinical cases, or purchased from the American Type Culture Collection (Manassas, VA), as well as kindly supplied by H. M. Hafez (Institute of Poultry Diseases, Berlin, Germany) and Vaxxinova Japan K.K. (Tokyo, Japan) ( Supplementary Table S1) [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29]. Besides FAdV-D and FAdV-E strains, we sequenced further strains of species FAdV-A (including the hitherto unavailable OTE reference genome) and FAdV-C (including, for the first time, the complete FAdV-10 genome), in order to increase robustness of the analysis.…”

Section: Virus Strains and Dna Preparationmentioning

confidence: 99%

Fowl Adenovirus (FAdV) Recombination with Intertypic Crossovers in Genomes of FAdV-D and FAdV-E, Displaying Hybrid Serological Phenotypes

Schachner

González

Endler

et al. 2019

Viruses

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After analyzing 27 new genomes from fowl adenovirus (FAdV) field isolates and so-far unsequenced prototypes, we report the first evidence for recombination in FAdVs. Recombination was confined to species FAdV-D and FAdV-E, accommodating the largest number of, and the intraspecies-wise most differentiated, types. The majority of detected events occurred in FAdV-E, involving segments with parental origin of all constitutive types. Together with the diversity of breakpoints, this suggests widespread recombination in this species. With possible constraints through species-specific genes and diversification patterns, the recombinogenic potential of FAdVs attains particular interest for inclusion body hepatitis (IBH), an important disease in chickens, caused by types from the recombination-prone species. Autonomously evolving, recombinant segments were associated with major sites under positive selection, among them the capsid protein hexon and fiber genes, the right-terminal ORFs 19, 25, and the ORF20/20A family. The observed mosaicism in genes indicated as targets of adaptive pressures points toward an immune evasion strategy. Intertypic hexon/fiber-recombinants demonstrated hybrid neutralization profiles, retrospectively explaining reported controversies on reference strains B3-A, T8-A, and X11-A. Furthermore, cross-neutralization supported sequence-based evidence for interdomain recombination in fiber and contributed to a tentatively new type. Overall, our findings challenge the purported uniformity of types responsible for IBH, urging more complete identification strategies for FAdVs. Finally, important consequences arise for in vivo studies investigating cross-protection against IBH.

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“…In addition, there are also reports of viral co-infection, such as by duck circovirus (DuCV) and duck hepatitis virus 1 (DHV-1) [18]; H9N2 AIV and Tembusu virus (TMUV) [19]; Marek’s disease virus (MDV) and CIAV [20]; and duck hepatitis A virus (DHAV-1) and DHAV-3 [21]. Currently, although FAdV-4 can clearly cause HHS on its own in chickens and ducks [22,23,24,25], both the extent of the FAdV-4 infection and degree of co-infection in the area remain unclear. Therefore, to better understand the prevalence of FAdV-4 and the incidence of co-infection by immunosuppressive viruses, an epidemiological survey of FAdV-4 infection in chickens and ducks with HHS in the province was conducted.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Fowl Adenovirus Serotype 4 and Co-Infection by Immunosuppressive Viruses in Fowl with Hydropericardium Hepatitis Syndrome in Shandong Province, China

Lin

et al. 2019

Viruses

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Fowl adenovirus serotype 4 (FAdV-4) is the pathogenic agent of hydropericardium hepatitis syndrome (HHS) in chickens and ducks, which has caused huge economic losses for the Chinese poultry industry since 2015. In order to objectively determine the prevalence and co-infection status of the virus in Shandong province in China, we analyzed a total of 679 clinical cases of chickens and ducks from 36 farms in the province. The results showed that the FAdV-4 infection rate was 65.2% (443/679), and the rate in breeder ducks was almost two-fold higher than that in breeder chickens (68.57% vs. 34.30%). Notably, co-infection by H9N2 avian influenza virus, infectious bursal disease virus, and/or chicken infectious anemia virus was very common in the 443 FAdV-4-positive cases. Furthermore, phylogenetic analysis of the hexon genes of four Shandong FAdV-4 isolates revealed that these strains clustered into Indian reference strains, indicating that the Shandong FAdV-4 strains might have originated in India. These findings provide the first data on the prevalence and co-infection status of FAdV-4 in Shandong province, which may serve as a foundation for the prevention of FAdV-4 in the field.

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Complete genome sequence and pathogenicity of fowl adenovirus serotype 4 involved in hydropericardium syndrome in Southwest China

Cited by 30 publications

References 42 publications

LAMP real-time turbidity detection for fowl adenovirus

LAMP real-time turbidity detection for fowl adenovirus

Fowl Adenovirus (FAdV) Recombination with Intertypic Crossovers in Genomes of FAdV-D and FAdV-E, Displaying Hybrid Serological Phenotypes

Prevalence of Fowl Adenovirus Serotype 4 and Co-Infection by Immunosuppressive Viruses in Fowl with Hydropericardium Hepatitis Syndrome in Shandong Province, China

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