Complete genome sequence analysis of a predominant infectious bronchitis virus (IBV) strain in China

Liu, Xing Li; Su, Jing; Zhao, Ji Xun; Zhang, Guo Zhong

doi:10.1007/s11262-008-0282-5

Cited by 50 publications

(48 citation statements)

References 39 publications

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“…These results suggest the need for dynamic and persistent surveillance of IBV in China. Liu et al [36] showed that the A2-like virus, which is a 4/91-serotyperelated virus determined by neutralization test, was the predominant IBV type in chicken flocks. However, more detailed results from the present study, our previous reports [15,23,26] tion against the LX4-type IBV [30] , which suggests a need to develop vaccines from LX-type local strains.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization and Pathogenicity of Infectious Bronchitis Coronaviruses: Complicated Evolution and Epidemiology in China Caused by Cocirculation of Multiple Types of Infectious Bronchitis Coronaviruses

et al. 2009

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Objective: To monitor and study the molecular epidemiology, evolution and pathogenicity of infectious bronchitis viruses (IBVs) in China in recent years and further our knowledge of the evolution of IBVs. Methods: Thirty-seven IBV isolates were isolated from commercial chickens in China. The isolates were characterized by RT-PCR, sequencing, typing and analyzing the entire S1 gene. In addition, 4 selected IBV isolates were used to experimentally infect the specific pathogen-free chickens to study their pathogenicity. Results: Three types of IBV have been cocirculating in chicken flocks in China in recent years. Unique insertions and deletions in S1 protein regions were identified among different types of IBV. Moreover, a new IBV strain was isolated and identified in a layer hen. S1 gene analysis showed that a recombination event had occurred in the virus’s evolutionary process. In addition, experimental infection has shown that IBV isolates have been nephropathogenic in China in recent years. Conclusion: Mutations, insertions, deletions and recombination of the S1 protein gene contribute to the genetic diversity of IBV in China. Cocirculation of multiple types of IBV in field conditions in China renders its epidemiology and evolution very complicated, indicating the necessity for development of new vaccines or vaccine strategies.

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Section: Discussionmentioning

confidence: 99%

Molecular Characterization and Pathogenicity of Infectious Bronchitis Coronaviruses: Complicated Evolution and Epidemiology in China Caused by Cocirculation of Multiple Types of Infectious Bronchitis Coronaviruses

et al. 2009

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“…Viral genome is around 27.6 kb in length [4,5] and contains a 5 0 cap and a 3 0 -polyadenylated tail. Its genome encodes four structural proteins: phosphorylated nucleocapsid protein (N), membrane glycoprotein (M), spike glycoprotein(S), and small membrane protein (E) [6,7].…”

Section: Introductionmentioning

confidence: 99%

Genetic analysis revealed LX4 genotype strains of avian infectious bronchitis virus became predominant in recent years in Sichuan area, China

et al. 2010

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Between 2008 and 2010, 19 strains of infectious bronchitis virus (IBV) were isolated from the vaccinated chicken flocks in Sichuan province, China. The S1 genes of the isolates were amplified and sequenced. Phylogenetic analysis revealed that the 19 isolates and 37 reference IBV strains can be grouped into eight genotypes. Although IBVs of Taiwan-I type, massachusetts type, and proventriculitis type were isolated, but most isolates were LX4 genotype. Homology analysis of the sequences of S1 genes of the 19 isolates and 37 reference IBV strains revealed that the identity of the nucleotides and amino acid sequences of the S1 genes between the 15 LX4-type isolates and other IBV strains were 71.9-99.3% and 72.1-99.1%, respectively, while those of the analyzed IBV of LX4 type were 96.0-99.9% and 94.3-99.8%, respectively. The results from this study and other published results in the GenBank database showed that isolates circulating in Sichuan province in recent years were mainly LX4 genotype, which is the predominant genotype circulated in China in recent years.

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“…The investigations in IBV isolated from immunized chickens discovered that immunity effect declined partly owing to the genetic variation in the process of IBV evolution [7,22,23,25,30] . We found that more than 40% IBVs belonged to LX4 type, which was circulating prevalently in chicken flocks in China, the result corresponded with the reported literature [20,22,30] .…”

Section: Discussionmentioning

confidence: 99%

“…IBV is an enveloped, non-segmented and singlestranded positive RNA virus with a genome around 27.6-kb in length [5][6][7] . The genome encodes four structural proteins, including spike glycoprotein (S), integral membrane glycoprotein (M), small membrane protein (E) and phosphorylated nucleocapsid (N) protein.…”

Section: Introductionmentioning

confidence: 99%

Çin’de Avian Enfeksiyöz Bronşitis Virüsünün Epidemiyolojisi ve Evrimsel Özellikleri

Wang¹,

Gao²,

Zheng³

et al. 2015

Kafkas Univ Vet Fak Derg

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To examine the epidemiology and evolutionary characteristics of avian infectious bronchitis virus (IBV) in mainland China, the S1 gene of 63 IBVs isolated after 1993 was amplified by reverse transcription (RT)-polymerase chain reaction (PCR) and sequenced. Our results showed that the sequence length of 63 IBV strains ranged from 1.608 to 1.635 nucleotide (nt). Compared to the published representative strains, the homology of S1 nt sequences and their deduced amino acid (aa) residues between these isolates and reference strains ranged from 74.6% to 99.9% and 71.7% to 100%, respectively. Analysis of S proteins demonstrated that 63 isolates had eight kinds of cleavage sites (RRFRR, RRIRR, RRSKR, RRTGR, RRHRR, HRRRR, RRSRR and RRLRR), and the amount of RRFRR and HRRRR reached 46. Furthermore, the study showed that there was no direct relation between cleavage sites and genotypes, and cleavage sites were unable to decide the pathology types. Compared to vaccine strain H120, S1 nt sequences of 63 isolates had four sites deleted or inserted frequently, and the S1 proteins had three hypervariable regions. A comprehensive study was also carried out to study the S1 nt sequences of 63 isolates, 210 published reference strains and 10 vaccine strains; the investigation showed that all these stains could be divided into two types including 11 kinds of genotypes by the phylogenetic analysis. However, 63 isolates belonged to Mass, American, LX4, LHLJ/95 I, LDT3/03, J, BJ and LDL/97 I genotypes, and the LX4 genotype was co-circulated dominantly in chicken flocks over an 18-year period. It indicated that there were several IBV genotypes with new changes circulating in China, emphasizing the importance of continued IBV surveillance. Çin'de Avian Enfeksiyöz Bronşitis Virüsünün Epidemiyolojisi ve Evrimsel Özellikleri Özet Avian Enfeksiyöz Bronşitis Virüsünün (IBV) epidemiyolojisini ve evrimsel ezelliklerini araştırmak amacıyla Çin'de 1993 yılından sonra izole edilen 63 IBV S1 geni ters transkriptaz (RT)-polimeraz zincir reaksiyonu (PCR) ile amlifiye edilerek sekansı yapıldı. Çalışma sonuçları 63 IBV suşunun sekans boyutunun 1.608 ile 1.635 nükleotide (nt) arasında değiştiğini gösterdi. Yayınlanmış benzer suşlar ile karşılaştırıldığında bu izolatlar ile referans suşları arasında S1 nt sekanslarının homolojisi ve açığa çıkan amino asit (aa) rezidüleri sırasıyla %74.6 ile %99.9 ve %71.7 ile %100 arasında değişti. S proteinlerinin analizi 63 izolatın 8 çeşit bölünme bölgesine (RRFRR, RRIRR, RRSKR, RRTGR, RRHRR, HRRRR, RRSRR ve RRLRR) sahip olduğunu ve RRFRR ve HRRRR miktarının 46'ya ulaştığını ortaya koydu. Ayrıca, bölünme bölgesi ile genotipler arasında doğrudan bir ilişki olmadığı ve bölünme bölgelerinin patoloji tiplerini belirlemediği tespit edildi. Aşı suşu H120 ile karşılaştırıldığında 63 izolatın S1 nt sekanslarının sıklıkla çıkarılmış veya eklenmiş 4 bölgeye sahip olduğu belirlendi. S1 proteinleri üç adet oldukça değişken bölgeye sahip olduğu gözlemlendi. 63 izolatın S1 nt sekansları, 210 yayınlanmış referans suşu ve 10 aşı suşu...

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Complete genome sequence analysis of a predominant infectious bronchitis virus (IBV) strain in China

Cited by 50 publications

References 39 publications

Molecular Characterization and Pathogenicity of Infectious Bronchitis Coronaviruses: Complicated Evolution and Epidemiology in China Caused by Cocirculation of Multiple Types of Infectious Bronchitis Coronaviruses

Molecular Characterization and Pathogenicity of Infectious Bronchitis Coronaviruses: Complicated Evolution and Epidemiology in China Caused by Cocirculation of Multiple Types of Infectious Bronchitis Coronaviruses

Genetic analysis revealed LX4 genotype strains of avian infectious bronchitis virus became predominant in recent years in Sichuan area, China

Çin’de Avian Enfeksiyöz Bronşitis Virüsünün Epidemiyolojisi ve Evrimsel Özellikleri

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