Complete genome sequence analysis of enterovirus 71 isolated from children with hand, foot, and mouth disease in Thailand, 2012–2014

Mauleekoonphairoj, John; Vongpunsawad, Sompong; Puenpa, Jiratchaya; Korkong, Sumeth; Poovorawan, Yong

doi:10.1007/s11262-015-1239-0

Cited by 12 publications

(13 citation statements)

References 25 publications

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“…55, 75, 76 A single case of recombination was detected in Cambodia; interestingly, the parent strain appeared to originate from Taiwan. Studies from Vietnam 55 and Thailand 77 also reported a low number of recombination events in their EV-A71 strains.…”

Section: Discussionmentioning

confidence: 99%

Molecular epidemiology of human enterovirus 71 at the origin of an epidemic of fatal hand, foot and mouth disease cases in Cambodia

Duong

Mey

Éloit

et al. 2016

Emerging Microbes & Infections

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Human enterovirus 71 (EV-A71) causes hand, foot and mouth disease (HFMD). EV-A71 circulates in many countries and has caused large epidemics, especially in the Asia-Pacific region, since 1997. In April 2012, an undiagnosed fatal disease with neurological involvement and respiratory distress occurred in young children admitted to the Kantha Bopha Children's Hospital in Phnom Penh, Cambodia. Most died within a day of hospital admission, causing public panic and international concern. In this study, we describe the enterovirus (EV) genotypes that were isolated during the outbreak in 2012 and the following year. From June 2012 to November 2013, 312 specimens were collected from hospitalized and ambulatory patients and tested by generic EV and specific EV-A71 reverse transcription PCR. EV-A71 was detected in 208 clinical specimens while other EVs were found in 32 patients. The VP1 gene and/or the complete genome were generated. Our phylogenetic sequencing analysis demonstrated that 80 EV-A71 strains belonged to the C4a subgenotype and 3 EV-A71 strains belonged to the B5 genotype. Furthermore, some lineages of EV-A71 were found to have appeared in Cambodia following separate introductions from neighboring countries. Nineteen EV A (CV-A6 and CV-A16), 9 EV B (EV-B83, CV-B3, CV-B2, CV-A9, E-31, E-2 and EV-B80) and 4 EV C (EV-C116, EV-C96, CV-A20 and Vaccine-related PV-3) strains were also detected. We found no molecular markers of disease severity. We report here that EV-A71 genotype C4 was the main etiological agent of a large outbreak of HFMD and particularly of severe forms associated with central nervous system infections. The role played by other EVs in the epidemic could not be clearly established.

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Section: Discussionmentioning

confidence: 99%

Molecular epidemiology of human enterovirus 71 at the origin of an epidemic of fatal hand, foot and mouth disease cases in Cambodia

Duong

Mey

Éloit

et al. 2016

Emerging Microbes & Infections

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“…This may be because EV-A71 usually invades the nervous and respiratory systems and leads to acute neurologic and respiratory diseases such as paralysis, encephalitis, aseptic meningitis, and pneumonedema [31]. HFMD EV-A71-associated neurological syndrome has increased in many countries during the past 5 years, especially in the Asia-Pacific region [32, 33]. In general, since the predominant virus serotype varies each year, the periodicity of HFMD may be complicated by interference between causative enterovirus serotypes [32, 33].…”

Section: Discussionmentioning

confidence: 99%

“…HFMD EV-A71-associated neurological syndrome has increased in many countries during the past 5 years, especially in the Asia-Pacific region [32, 33]. In general, since the predominant virus serotype varies each year, the periodicity of HFMD may be complicated by interference between causative enterovirus serotypes [32, 33]. A limitation of our study is that we did not test other enterovirus serotypes, such as CV-A6 or CV-A10, which are becoming more predominant in some provinces of southern China [34].…”

Section: Discussionmentioning

confidence: 99%

Hand, Foot, and Mouth Disease in Hunan Province, China, 2009-2014: Epidemiology and Death Risk Factors

Luo

Gao

et al. 2016

PLoS ONE

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Hand, foot, and mouth disease (HFMD) is an arising public health problem in Asia, including China. Epidemiological data is necessary to enable judicious public health responses and interventions. We analyzed the epidemiological and laboratory data of 759,301 HFMD cases reported to the Hunan Provincial Center for Disease Control and Prevention from 1 January 2009 to 31 December 2014. Univariate and multivariable conditional logistic regression analyses were used to identify risk factors of fatality in HFMD. The incidence of HFMD was highest among children aged 1–3 years, compared with other age groups. Of the total HFMD cases, 7,222 (0.95%) were considered severe and 338 (0.04%) were fatal. Enterovirus-A71 was the major cause of severe and fatal cases (65.75% and 88.78%, respectively). For severe cases, the median time from symptom onset to diagnosis was 0.5 days (interquartile range [IQR] 0–1.5 days); the median time from diagnosis to severe illness was 2 days (IQR 1–3 days). For fatal cases, the median time from symptom onset to diagnosis was 0.5 days (IQR 0–1.5 days); the median time from diagnosis to death was 1.5 days (IQR 0.5–2.5 days). In multivariable analysis, the abuse of antibiotic, glucocorticoid and pyrazolone in village clinics at basic medical institutions were identified as independent risk factors for HFMD fatal cases. In conclusion, our results suggest that the future direction to control and respond to HFMD is intensive surveillance of enterovirus-A71 and improving the ability to diagnose disease and treat patients, especially in basic medical institutions.

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“…The first RT-PCR identifies enterovirus A71 (EV-A71), CV-A6, and CV-A16 ( 5 ). We then subjected virus-positive samples to full-length virus capsid protein 1 (VP1) gene amplification by using a conventional RT-PCR and nucleotide sequencing to identify EV-A71 subgenogroups ( 6 ). The second RT-PCR is a pan-enterovirus real-time RT-PCR, which also detects the glyceraldehyde-3-phosphate dehydrogenase gene (internal control).…”

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confidence: 99%

Enterovirus A71 Infection, Thailand, 2017

Puenpa¹,

Auphimai²,

Korkong³

et al. 2018

Emerg. Infect. Dis.

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Complete genome sequence analysis of enterovirus 71 isolated from children with hand, foot, and mouth disease in Thailand, 2012–2014

Abstract: The complete genome sequence of the enterovirus 71 strain CSF15/YN/ CHN/2013, first isolated from cerebrospinal fluid of a child in Yunnan, China, in 2013, was determined. According to the phylogenetic and homogeneity analyses, the isolate was assigned to subgenotype C4a.

Cited by 12 publications

References 25 publications

Molecular epidemiology of human enterovirus 71 at the origin of an epidemic of fatal hand, foot and mouth disease cases in Cambodia

Molecular epidemiology of human enterovirus 71 at the origin of an epidemic of fatal hand, foot and mouth disease cases in Cambodia

Hand, Foot, and Mouth Disease in Hunan Province, China, 2009-2014: Epidemiology and Death Risk Factors

Enterovirus A71 Infection, Thailand, 2017

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