Complete Genome Sequence Analysis of a Reassortant Strain of Bluetongue Virus Serotype 16 from Italy

Lorusso, Alessio; Costessi, Adalberto; Pirovano, Walter; Marcacci, Maurilia; Cammà, Cesare; Savini, Giovanni

doi:10.1128/genomea.00622-13

Cited by 6 publications

(4 citation statements)

References 14 publications

(16 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite the absence of any vaccination against this serotype and specific movement control policies, BTV‐16 virus circulation was quite limited in few geographical areas of Italy. The reasons behind the limited spread of this serotype are not fully clear (Lorusso et al., ).…”

Section: Assessmentmentioning

confidence: 99%

Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): bluetongue

More¹,

Bicout²,

Bøtner³

et al. 2017

EFS2

Self Cite

View full text Add to dashboard Cite

A specific concept of strain was developed in order to classify the BTV serotypes ever reported in Europe based on their properties of animal health impact: the genotype, morbidity, mortality, speed of spread, period and geographical area of occurrence were considered as classification parameters. According to this methodology the strain groups identified were (i) the BTV strains belonging to serotypes BTV-1-24, (ii) some strains of serotypes BTV-16 and (iii) small ruminant-adapted strains belonging to serotypes BTV-25, -27, -30. Those strain groups were assessed according to the criteria of the Animal Health Law (AHL), in particular criteria of Article 7, Article 5 on the eligibility of bluetongue to be listed, Article 9 for the categorisation according to disease prevention and control rules as in Annex IV and Article 8 on the list of animal species related to bluetongue. The assessment has been performed following a methodology composed of information collection, expert judgement at individual and collective level. The output is composed of the categorical answer, and for the questions where no consensus was reached, the different supporting views are reported. The strain group BTV (1-24) can be considered eligible to be listed for Union intervention as laid down in Article 5(3) of the AHL, while the strain group BTV-25-30 and BTV-16 cannot. The strain group BTV-1-24 meets the criteria as in Sections 2 and 5 of Annex IV of the AHL, for the application of the disease prevention and control rules referred to in points (b) and (e) of Article 9(1) of the AHL. The animal species that can be considered to be listed for BTV-1-24 according to Article 8(3) are several species of Bovidae, Cervidae and Camelidae as susceptible species; domestic cattle, sheep and red deer as reservoir hosts, midges insect of genus Culicoides spp. as vector species.

show abstract

Section: Assessmentmentioning

confidence: 99%

Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): bluetongue

More¹,

Bicout²,

Bøtner³

et al. 2017

EFS2

Self Cite

View full text Add to dashboard Cite

show abstract

“…Information based on partial genome sequences from a limited numbers of BTV field strains, indicated that reassortment between some strains had occurred (e.g. for BTV-16(e) in Italy 2002 or BTV-1(w) and BTV-4(w) in Sardinia 2012 [ 38 – 40 ]).…”

Section: Introductionmentioning

confidence: 99%

Widespread Reassortment Shapes the Evolution and Epidemiology of Bluetongue Virus following European Invasion

et al. 2015

View full text Add to dashboard Cite

Genetic exchange by a process of genome-segment ‘reassortment’ represents an important mechanism for evolutionary change in all viruses with segmented genomes, yet in many cases a detailed understanding of its frequency and biological consequences is lacking. We provide a comprehensive assessment of reassortment in bluetongue virus (BTV), a globally important insect-borne pathogen of livestock, during recent outbreaks in Europe. Full-genome sequences were generated and analysed for over 150 isolates belonging to the different BTV serotypes that have emerged in the region over the last 5 decades. Based on this novel dataset we confirm that reassortment is a frequent process that plays an important and on-going role in evolution of the virus. We found evidence for reassortment in all ten segments without a significant bias towards any particular segment. However, we observed biases in the relative frequency at which particular segments were associated with each other during reassortment. This points to selective constraints possibly caused by functional relationships between individual proteins or genome segments and genome-wide epistatic interactions. Sites under positive selection were more likely to undergo amino acid changes in newly reassorted viruses, providing additional evidence for adaptive dynamics as a consequence of reassortment. We show that the live attenuated vaccines recently used in Europe have repeatedly reassorted with field strains, contributing to their genotypic, and potentially phenotypic, variability. The high degree of plasticity seen in the BTV genome in terms of segment origin suggests that current classification schemes that are based primarily on serotype, which is determined by only a single genome segment, are inadequate. Our work highlights the need for a better understanding of the mechanisms and epidemiological consequences of reassortment in BTV, as well as other segmented RNA viruses.

show abstract

“…As for Seg‐6, 11 putative nucleotypes (A–K) are potentially designated but its sequence variation does not show an absolute correlation with serotype (Maan et al., ; Schulz et al., ; Sun et al., ). Importantly, a characteristic of BTV is the ability to “reassort” genomic segments with related members of the same species (Lorusso, Costessi et al., ; Lorusso, Sghaier et al., ; Nomikou et al., ). BTV strains derived from modified live‐attenuated vaccines (MLVs) have also been used for BT prevention and isolated in the field (Batten, Maan, Shaw, Maan, & Mertens, ; Ferrari et al., ; Savini et al., ).…”

Section: Introductionmentioning

confidence: 99%

A novel Bluetongue virus serotype 3 strain in Tunisia, November 2016

Sghaier

Lorusso

Portanti

et al. 2017

Transbound Emerg Dis

View full text Add to dashboard Cite

Since 1998, southern Europe has experienced multiple incursions of different serotypes and topotypes of Bluetongue virus, a vector-borne transmitted virus, the causative agent of Bluetongue (BT), a major disease of ruminants. Some of these incursions originated from northern Africa, likely because of wind-blown dissemination of infected midges. In this report, we describe the detection and whole genome characterization of a novel BTV-3 strain identified in a symptomatic sheep in Tunisia. Sequences were immediately deposited with the GenBank Database under Accession Nos KY432369-KY432378. Alert and preparedness are requested to face the next vector seasons in northern Africa and the potential incursion of this novel strain in southern Europe.

show abstract

Complete Genome Sequence Analysis of a Reassortant Strain of Bluetongue Virus Serotype 16 from Italy

Cited by 6 publications

References 14 publications

Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): bluetongue

Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): bluetongue

Widespread Reassortment Shapes the Evolution and Epidemiology of Bluetongue Virus following European Invasion

A novel Bluetongue virus serotype 3 strain in Tunisia, November 2016

Contact Info

Product

Resources

About