Complete Genome Sequence Analysis of Human Echovirus Type 30 Isolated in China

Han, Jianfeng; Xu, Lijuan; Cao, Ruiyuan; Zhao, Hui; Jiang, Tao; Deng, Yong-Qiang; Li, Yuexiang; Zhu, Shun-Ya; Yu, Man; Qin, E-De; Qin, Cheng‐Feng

doi:10.1128/jvi.02672-12

Cited by 2 publications

(2 citation statements)

References 14 publications

(12 reference statements)

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“…Similarity plot and bootscanning analysis was performed by using the Simplot 3.5.1 program with a 400 nucleotide window moving in 20 nt steps and a Jukes–Cantor correction 42 43 . The EV-B83 prototype strain USA/CA76-10392 and other closely related sequences 44 45 46 47 48 used in the recombination analysis were listed in Table 3 .…”

Section: Methodsmentioning

confidence: 99%

Complete Genome Analysis of an Enterovirus EV-B83 Isolated in China

Tang

Tian

et al. 2016

Sci Rep

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Enterovirus B83 (EV-B83) is a recently identified member of enterovirus species B.It is a rarely reported serotype and up to date, only the complete genome sequence of the prototype strain from the United States is available. In this study, we describe the complete genomic characterization of an EV-B83 strain 246/YN/CHN/08HC isolated from a healthy child living in border region of Yunnan Province, China in 2008. Compared with the prototype strain, it had 79.6% similarity in the complete genome and 78.9% similarity in the VP1 coding region, reflecting the great genetic divergence among them. VP1-coding region alignment revealed it had 77.2-91.3% with other EV-B83 sequences available in GenBank. Similarity plot analysis revealed it had higher identity with several other EV-B serotypes than the EV-B83 prototype strain in the P2 and P3 coding region, suggesting multiple recombination events might have occurred. The great genetic divergence with previously isolated strains and the extremely rare isolation suggest this serotype has circulated at a low epidemic strength for many years. This is the first report of complete genome of EV-B83 in China.

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Section: Methodsmentioning

confidence: 99%

Complete Genome Analysis of an Enterovirus EV-B83 Isolated in China

Tang

Tian

et al. 2016

Sci Rep

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“…The full-length genome of 2012EM161 comprised 7427 bp and included a 741 bp 5′ UTR, a 101 bp 3′ UTR and an open reading frame encoding a 2194 amino acid polyprotein, the cleavage site of which was consistent with JX854435 [ 6 ]. The phylogenetic tree based on the VP1 region showed that 2012EM161 was located in the cluster 1 of h lineage of E30 (Fig.…”

Section: Sequence Propertiesmentioning

confidence: 99%

Complete genome sequence analysis of human echovirus 30 isolated during a large outbreak in Guangdong Province of China, in 2012

Xiao

Huang

et al. 2013

Arch Virol

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In May and June 2012, an outbreak of aseptic meningitis caused by Echovirus 30 (E30) occurred on a large scale in Luoding, Guangdong Province, China. Our team successfully isolated one subtype, strain 2012EM161, and its complete genome was sequenced. The phylogenetic tree of viral protein (VP) 1 gene sequences showed that the viral isolate was similar to the E30 strain prevalent in Fujian (2011), with identity of 98.05–99.32 % and 98.63–99.32 % for nucleotides and amino acids respectively. Whole genome-based phylogenetic analysis indicated that 2012EM161 contained the most proximate consensus to DQ246620 (Zhejiang, 2003) and FDJS03 (AY948442, Jiangsu, 2005), with nucleotide homogeneity of 87.09 % and 86.98 % respectively. The RDP4.16 and Simplot analysis showed that the newly discovered 2012EM161 was probably a recombinant, which was closely related to the strain of E30 (DQ246620) in the first half of the genome and the strain of E6 (JX976771) in genomic P3 region. The whole genome sequence of 2012EM161 will allow further study of the origin, evolution, and the molecular epidemiology of E30 strains.Electronic supplementary materialThe online version of this article (doi:10.1007/s00705-013-1818-0) contains supplementary material, which is available to authorized users.

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Complete Genome Sequence Analysis of Human Echovirus Type 30 Isolated in China

Cited by 2 publications

References 14 publications

Complete Genome Analysis of an Enterovirus EV-B83 Isolated in China

Complete Genome Analysis of an Enterovirus EV-B83 Isolated in China

Complete genome sequence analysis of human echovirus 30 isolated during a large outbreak in Guangdong Province of China, in 2012

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