Complete Genome Resources for <i>Ralstonia</i> Bacterial Wilt Strains UW763 (Phylotype I); Rs5 and UW700 (Phylotype II); and UW386, RUN2474, and RUN2279 (Phylotype III)

Steidl, Olivia; Truchon, Alicia N.; Hayes, Madeline M.; Allen, Caitilyn

doi:10.1094/mpmi-04-21-0086-a

Cited by 6 publications

(2 citation statements)

References 20 publications

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“…The strains used in these studies are described in Table 3 . The RSSC has been subdivided into four phylotypes and 3 genomospecies ( 37 , 38 ). Four R3bv2 R. solanacearum strains isolated from diverse geographical locations were chosen to represent the SA subgroup.…”

Section: Methodsmentioning

confidence: 99%

“…The practical use of two combined disinfestation methods was informally assessed in collaboration with a large commercial tomato producer in Senegal suffering a widespread outbreak of bacterial wilt. R. solanacearum strain UW763 (phylotype I, sequevar 14; whole-genome sequence at NCBI Bioproject number PRJNA591018 [ 38 ]) was isolated from the surface-sterilized stem of a symptomatic tomato plant from this production facility. The farm’s irrigation system and water from the nearby river that was used as an irrigation source contained very similar R. solanacearum strains, as determined by the sequences of the diagnostic egl gene.…”

Section: Methodsmentioning

confidence: 99%

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Validating Methods To Eradicate Plant-Pathogenic Ralstonia Strains Reveals that Growth In Planta Increases Bacterial Stress Tolerance

et al. 2022

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Validating Methods To Eradicate Plant-Pathogenic Ralstonia Strains Reveals that Growth In Planta Increases Bacterial Stress Tolerance

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Diverse interactions of five core type III effectors from Ralstonia solanacearum with plants

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Xiong

et al. 2023

Journal of Genetics and Genomics

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First Report of Ralstonia pseudosolanacearum Causing Wilt Disease in Tomato (Solanum lycopersicum) Plants in Mexico

et al. 2023

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Mexico produces more than four million tons of tomato fruits and ranks tenth worldwide. In February 2022, tomato plants in a greenhouse in Culiacan, Sinaloa State, were affected by wilt diseases with an incidence of 20% and irreversible wilt and death of the infected plants (severity up 70%). When cut stems from affected plants, a reddish to brown discoloration of the vascular system was observed and these were disinfected with 1% NaClO for 5 min and then placed in a humid chamber. Characteristic milky-white exudate was obtained. From that exudate, irregular, mucoid, and white colonies with pink centres were obtained on casamino peptone glucose (CPG) plates supplemented with 1% 2,3,5-triphenyl 15 tetrazolium chloride (TZC); these characteristics are typical of the Ralstonia solanacearum species complex (RSSC) (Garcia et al., 2019). Identification of the pathogen was done by PCR using specific primer pairs reported by Paudel et al. (2022), RssC-wF3 (5’-TATATATCCTCGACTTTTCCATGAAGCTGTG-3’) - RssCwR3 (5’-CTATATATATACCCCACTTGTTGAGGAACTG-3’) and Rpseu-wF5 (5’-TTTTATTTTTTTGGTGTCCGGGCCAAGATAG-3’) - Rpseu-wR5 (5’- TTATATTACTCGAACGTGCTGCAAAACCACT-3’), which amplified fragments of 162 and 251 bp for RSSC and Ralstonia pseudosolanacearum, respectively. Additionally, 759 (5’-GTCGCCGTCAACTCACTTTCC-3’) - 760 (5’-GTCGCCGTCAGCAATGCGGAATCG-3’) (Opina, et al., 1997) and Nmult21:1F (5′-CGTTGATGAGGCGCGCAATTT-3′) - Nmult22:RR (5’- TCGCTTGACCCTATAACGAGTA-3’) (Fegan and Prior, 2005) were used to generate 282 and 144 bp amplicons for RSSC and phylotype I, respectively. Subsequen to making the specific detection, the representative strain ClnMx was used to generate a sequence for the endoglucanase (egl) gene for separation into sequevars by using the primers Endo-F (5′- ATGCATGCCGCTGGTCGCCGC-3′) and Endo-R (5′-GCGTTGCCCGGCACGAACACC-3′), which amplified a fragment of 750 bp (Fegan et al., 1998). The egl sequence (GenBank Access ON542479) showed 100% identity with the well-defined R. pseudosolanacearum sequevar 14, which was isolated from tomato plants from Senegal (UW763, I-14 GenBank Access CP051174) (Steidl et al., 2021), as well as, the strain MAFF 301070 (GenBank Access AB508612) from Japanese tomato. For pathogenicity tests, four 1-month-old tomato plants were infected using an insulin syringe that contained a pure bacterial suspension with approximately 2x108 CFU/mL. For each plant, 20 µL was infiltrated into the axil of the third upper leaf, and for untreated controls, tomato plants were infiltrated with sterile water. All plants were kept at 28°C under greenhouse conditions. Symptoms resembling those observed in the field were observed in inoculated plants six days after inoculation, and the plant pathogen was recovered on TZC medium. To confirm the bacteria identification a PCR using the specific primer pairs mentioned early was carried out. In contrast, water-treated control plants remained healthy. Koch’s postulates were carried out twice with similar results. Ralstonia solanacearum species complex (RSSC) causes severe economic losses in many countries of the world because of their capability to infect a wide range of host plants, including potato, tomato, eggplant, tobacco, and, banana, among others. Ralstonia pseudosolanacearum has been reported to cause tomato wilt disease mainly on the Afro-Eurasian continent in areas such as Senegal, Cambodia, and Japan (Klass et al., 2019). To our knowledge, this is the first report of R. pseudosolanacearum causing bacterial wilt diseases in tomato plants from Mexico and because, the control of this bacteria is a challenge by the long survival time in soil, water, and infected plant tissues, the identification of this important pathogen could provide relevant information for developing management strategies.

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Complete Genome Resources for Ralstonia Bacterial Wilt Strains UW763 (Phylotype I); Rs5 and UW700 (Phylotype II); and UW386, RUN2474, and RUN2279 (Phylotype III)

Cited by 6 publications

References 20 publications

Validating Methods To Eradicate Plant-Pathogenic Ralstonia Strains Reveals that Growth In Planta Increases Bacterial Stress Tolerance

Validating Methods To Eradicate Plant-Pathogenic Ralstonia Strains Reveals that Growth In Planta Increases Bacterial Stress Tolerance

Diverse interactions of five core type III effectors from Ralstonia solanacearum with plants

First Report of Ralstonia pseudosolanacearum Causing Wilt Disease in Tomato (Solanum lycopersicum) Plants in Mexico

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