Complete Genome of Staphylococcus aureus Phage SA11

Kim, Min Soo; Myung, Heejoon

doi:10.1128/jvi.01574-12

Cited by 22 publications

(18 citation statements)

References 11 publications

(8 reference statements)

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“…These dimensions correspond to those of other twortlikeviruses infecting S. aureus (5). Consequently, phage SA11, closely related to Romulus and Remus, was presumably erroneously described as a siphovirus (14).…”

Section: Isolation and Morphologymentioning

confidence: 99%

See 1 more Smart Citation

Romulus and Remus, Two Phage Isolates Representing a Distinct Clade within the Twortlikevirus Genus, Display Suitable Properties for Phage Therapy Applications

Vandersteegen

Kropinski

Nash

et al. 2013

J Virol

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eThe renewed interest in controlling Staphylococcus aureus infections using their natural enemies, bacteriophages, has led to the isolation of a limited number of virulent phages so far. These phages are all members of the Twortlikevirus, displaying little variance. We present two novel closely related (95.9% DNA homology) lytic myoviruses, Romulus and Remus, with double-stranded DNA (dsDNA) genomes of 131,333 bp and 134,643 bp, respectively. Despite their relatedness to Staphylococcus phages K, G1, ISP, and Twort and Listeria phages A511 and P100, Romulus and Remus can be proposed as isolates of a new species within the Twortlikevirus genus. A distinguishing feature for these phage genomes is the unique distribution of group I introns compared to that in other staphylococcal myoviruses. In addition, a hedgehog/intein domain was found within their DNA polymerase genes, and an insertion sequence-encoded transposase exhibits splicing behavior and produces a functional portal protein. From a phage therapy application perspective, Romulus and Remus infected approximately 70% of the tested S. aureus isolates and displayed promising lytic activity against these isolates. Furthermore, both phages showed a rapid initial adsorption and demonstrated biofilm-degrading capacity in a proof-of-concept experiment.

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Section: Isolation and Morphologymentioning

confidence: 99%

“…This genus includes staphylococcal phages K (7), G1, Twort (8), Sb-1 (9), ISP (10,11), A5-W, Staph1N, Fi200W, P4W, 676Z, A3R, MSA6 (12,13), SA11 (14), and GH15 (15). Their double-stranded genomes contain 127,188 bp to 140,194 bp, display a GϩC content of 30.04 to 30.60%, and contain 183 to 217 open reading frames (ORFs).…”

mentioning

confidence: 99%

Romulus and Remus, Two Phage Isolates Representing a Distinct Clade within the Twortlikevirus Genus, Display Suitable Properties for Phage Therapy Applications

Vandersteegen

Kropinski

Nash

et al. 2013

J Virol

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“…The results showed that the Staphylococcus phages K, G1, Twort, A5W, Sb-1, ISP, SA5, GH15, JD007, SA11, vB_SauM_Remus, vB_SauM_Romulus, S25-3, S25-4, phiIPLA-RODI, phiIPLA-C1C, phiSA012, Team1, P108, MCE-2014, 812, and SA1 belong to the Myoviridae family and are all lytic phages789101112131415. The previous report shows that phage phiIPLA-RODI and phiIPLA-C1C are lytic phages11, our results were consistent with the studies reported previously.…”

Section: Resultsmentioning

confidence: 99%

“…Published data show that they have a wide host range and can potentially be used for phage therapy. To date, the complete genome sequences of dozens of Staphylococcus phages of the Myoviridae family have become available, including phage K, G1, Twort, A5W, Sb-1, ISP, SA5, GH15, JD007, SA11, vB_SauM_Remus, vB_SauM_Romulus, S25-3, S25-4, phiIPLA-RODI, phiIPLA-C1C, phiSA012, Team1, P108, MCE-2014, 812, SA1, Staphy1N, MSA6, 676Z, P4W, and Fi200w789101112131415. These phages can potentially be used for the treatment of infectious diseases caused by Staphylococcus aureus in animal models.…”

mentioning

confidence: 99%

Safety assessment of Staphylococcus phages of the family Myoviridae based on complete genome sequences

Cui

Guo

Dong

et al. 2017

Sci Rep

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Staphylococcus phages of the Myoviridae family have a wide host range and potential applications in phage therapy. In this report, safety assessments of these phages were conducted based on their complete genome sequences. The complete genomes of Staphylococcus phages of the Myoviridae family were analyzed, and the Open Reading Frame (ORFs) were compared with a pool of virulence and antibiotic resistance genes using the BLAST algorithm. In addition, the lifestyle of the phages (virulent or temperate) was also confirmed using PHACTS. The results showed that all phages were lytic and did not contain resistance or virulence genes based on bioinformatic analyses, excluding the possibility that they could be vectors for the dissemination of these undesirable genes. These findings suggest that the phages are safe at the genome level. The SceD-like transglycosylase, which is a biomarker for vancomycin-intermediate strains, was widely distributed in the phage genomes. Approximately 70% of the ORFs encoded in the phage genomes have unknown functions; therefore, their roles in the antibiotic resistance and virulence of Staphylococcus aureus are still unknown and require consideration before use in phage therapy.

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“…As shown, phage genetics vary among phages of different hosts, even in an interlinked supply chain like the industry of food processing [24][25][26][27][28][29]. The main bottleneck for functional overall genomics, including phage genomics, is the low number of available genomes and described genes (open reading frames or ORFs).…”

Section: Phage Genome Sequencing Focusing On Economic Applications Inmentioning

confidence: 99%

Bacteriophage Genome Sequencing: A New Alternative to Understand Biochemical Interactions between Prokaryotic Cells and Phages

G¹,

B²,

A³

et al. 2017

J Microb Biochem Technol

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Complete Genome of Staphylococcus aureus Phage SA11

Cited by 22 publications

References 11 publications

Romulus and Remus, Two Phage Isolates Representing a Distinct Clade within the Twortlikevirus Genus, Display Suitable Properties for Phage Therapy Applications

Romulus and Remus, Two Phage Isolates Representing a Distinct Clade within the Twortlikevirus Genus, Display Suitable Properties for Phage Therapy Applications

Safety assessment of Staphylococcus phages of the family Myoviridae based on complete genome sequences

Bacteriophage Genome Sequencing: A New Alternative to Understand Biochemical Interactions between Prokaryotic Cells and Phages

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