Complete Genome Insights into Lactococcus petauri CF11 Isolated from a Healthy Human Gut Using Second- and Third-Generation Sequencing

Ou, Yun-Jing; Ren, Qingyun; Fang, Shun; Wu, Jiguo; Jiang, Yunxia; Chen, Yi-ran; Zhong, Yong; Wang, Dedong

doi:10.3389/fgene.2020.00119

Cited by 15 publications

(14 citation statements)

References 36 publications

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“…petauri LZys1, which have been analysed in this study, and other L. petauri strains such as CF11 [15] and LG_SAV_20 [16] allowed us to annotate the genome of L. petauri LZys1 and make further investigations.…”

Section: Introductionmentioning

confidence: 99%

“…Meanwhile, probiotic associated genome analysis of multiple strains could reveal the evolutionary process and provide insights into genetic properties based on the genome map and sequences. The whole-genome sequences of L. petauri LZys1, which have been analysed in this study, and other L. petauri strains such as CF11 [15] and LG_SAV_20 [16] allowed us to annotate the genome of L. petauri LZys1 and make further investigations.…”

Section: Introductionmentioning

confidence: 99%

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Complete genome sequence and probiotic properties of Lactococcus petauri LZys1 isolated from healthy human gut

Ouyang

Zhang

Wang

et al. 2021

Journal of Medical Microbiology

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Introduction. Lactococcus petauri LZys1 ( L. petauri LZys1) is a type of lactic acid bacteria (LAB), which was initially isolated from healthy human gut. Hypothesis/Gap Statement. It was previously anticipated that L. petauri LZys1 has potential characteristics of probiotic properties. The genetic structure and the regulation functions of L. petauri LZys1 need to be better revealed. Aim. The aim of this study was to detect the probiotic properties L. petauri LZys1 and to reveal the genome information related to its genetic adaptation and probiotic profiles. Methodology. Multiple in vitro experiments were carried out to evaluate its lactic acid-producing ability, resistance to pathogenic bacterial strains, auto-aggregation and co-aggregation ability, and so on. Additionally, complete genome sequencing, gene annotation, and probiotic associated gene analysis were performed. Results. The complete genome of L. petauri LZys1 comprised of 1 985 765 bp, with a DNA G+C content of 38.07 %, containing 50 tRNA, seven rRNA, and four sRNA. A total of 1931 genes were classified into six functional categories by Kyoto Encyclopaedia of Genes and Genomes (KEGG) database. The neighbour-joining phylogeny tree based on the whole genome of L. petauri LZys1 and other probiotics demonstrated that L. petauri LZys1 has a significant similarity to Lactococcus garvieae . The functional genes were detected to expound the molecular mechanism and biochemical processes of its potential probiotic properties, such as atpB gene. Conclusion. All the results described in this study, together with relevant information previously reported, made L. prtauri LZys1 a very interesting potential strain to be considered as a prominent candidate for probiotic use.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Complete genome sequence and probiotic properties of Lactococcus petauri LZys1 isolated from healthy human gut

Ouyang

Zhang

Wang

et al. 2021

Journal of Medical Microbiology

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“…Some isolates recovered from the same farm exhibited different antimicrobial resistance phenotypes. Although the isolates were genetically similar according to MLST (same has been previously described (Kotzamanidis et al, 2020;Ou et al, 2020) and will be further assessed in the genome of some isolates from this study for comparison with the phenotypic profile of the isolates.…”

Section: Discussionmentioning

confidence: 95%

Emerging fish pathogens Lactococcus petauri and L. garvieae in Nile tilapia (Oreochromis niloticus) farmed in Brazil

Egger¹,

Rosa²,

Pádua³

et al. 2022

Preprint

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Lactococcosis in fish has been associated with Lactococcus garvieae and the recently described L. petauri. However, the relevance of these emerging fish pathogens to Nile tilapia still requires thorough understanding. This study investigated lactococcosis outbreaks in Nile tilapia on Brazilian farms and characterized the isolates through molecular identification of the bacterial species, multilocus sequence typing (MLST) analysis, virulence to Nile tilapia, and antimicrobial susceptibility. Lactococcosis outbreaks were monitored from 2019 to 2022 throughout Brazil. The outbreaks occurred mainly during warmer months, and co-infections were observed in four farms, whereas concurrent bacterial infections were identified in all farms. Since the sequence of the 16S rRNA was not capable of differentiating between L. petauri and L. garvieae, Lactococcus spp. isolates were identified at the species level using the gyrB gene sequence. In total, 30 isolates were classified as L. petauri and two as L. garvieae. All L. petauri isolates were grouped in ST24, except for one isolate which belonged to the newly described ST47. A new ST was also described for the L. garvieae isolates identified, ST46. Furthermore, L. petauri ST24 and ST47 were characterized as singletons, whereas L. garvieae ST46 was grouped with ST16 and ST17 and formed CC17. For the challenge trial, an L. petauri ST24 isolate was chosen considering that this MLST lineage was the most frequently observed. L. petauri was reisolated from challenged Nile tilapia, confirming the pathogenicity of this bacterium to Nile tilapia. The infection in the fish progressed very rapidly, and within 48 h post-challenge clinical signs and the first mortalities were observed. The estimated LD50 was 5.74 x 103 CFU 15 days post-challenge. Provisional epidemiological cutoff values were determined for L. petauri for six antimicrobial agents from different drug classes. All isolates were characterized as wild type (WT) for neomycin and oxytetracycline, whereas 96.67 % of the isolates were characterized as WT for amoxicillin, erythromycin, and florfenicol, and 83.33 % were WT for norfloxacin. Of the 14 outbreaks analyzed, 12 were caused by L. petauri and two by L. garvieae. The gyrB gene sequence was used to differentiate L. petauri from L. garvieae and allowed for the correct identification of these pathogens. Two MSLT lineages of L. petauri were identified and ST24 was observed in different regions of the country, illustrating a rapid expansion of this bacterial lineage.

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“…SKP K.4 isolate belongs to L. petauri species. This bacterium was successfully isolated from a healthy human gut (Ou et al 2020). L. petauri has the closest relationship with L. garvieae and is a Gram-positive, non-motile, nonspore forming, and facultatively anaerobic (Goodman et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity of lactic acid bacteria isolated from Sumbawa horse milk, Indonesia

Kusdianawati¹,

Mustopa

Fatimah

et al. 2020

Biodiversitas

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Abstract. Kusdianawati, Mustopa AZ, Fatimah, Budiarto BR. 2020. Genetic diversity of lactic acid bacteria isolated from Sumbawa horse milk, Indonesia. Biodiversitas 21: 3225-3233. LAB from Sumbawa horse milk has good potential antimicrobial and probiotic agents. It is known, the study on LAB diversity based on its phenotypic characters is difficult to be distinguished. However, the development of molecular characterization based on the genotypic characteristic could be done for LAB diversity analysis. The aim of this study is to obtain the genetic diversity of LAB from Sumbawa horse milk collected from Penyaring Village and Lennanguar Village, Sumbawa, West Nusa Tenggara Indonesia. LAB strains were identified based on their genotypic characteristics, including their randomly amplified polymorphic DNA (RAPD) primers profiles and 16S ribosomal RNA (rRNA) sequences. The result of RAPD-PCR analysis showed 5 clusters of dendrograms resulted from GTG5 and LB2 primer amplification. Based on 16 rRNA sequences result, the phylogenetic tree was constructed and revealed 7 species of LAB i.e: SK 1.5, SKP K.3, SKP K.5, SKP K.9/SKP K.7/M.SKP K.3, SKL K.4, M.SKL K.1/ M.SKL K.5, and SKP K.4 belonging to the species of Enterococcus faecium, Weissella confusa, Lactococcus garvieae, Enterococcus thailandicus, Lactobacillus fermentum, Enterococcus faecalis, and Lactococcus petauri. In this study, the bacteria from Enterococcus sp., Lactococcus garvieae, and Lactococcus petauri existed as a novel of bacteria which means they have not been isolated and identified in Sumbawa horse milk compared to the previous findings.

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Complete Genome Insights into Lactococcus petauri CF11 Isolated from a Healthy Human Gut Using Second- and Third-Generation Sequencing

Cited by 15 publications

References 36 publications

Complete genome sequence and probiotic properties of Lactococcus petauri LZys1 isolated from healthy human gut

Complete genome sequence and probiotic properties of Lactococcus petauri LZys1 isolated from healthy human gut

Emerging fish pathogens Lactococcus petauri and L. garvieae in Nile tilapia (Oreochromis niloticus) farmed in Brazil

Genetic diversity of lactic acid bacteria isolated from Sumbawa horse milk, Indonesia

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