Complete Genome and Clinicopathological Characterization of a Virulent Newcastle Disease Virus Isolate from South America

Diel, Diego G.; Susta, Leonardo; Cardenas-Garcia, Stivalis; Killian, Mary Lea; Brown, Corrie C.; Miller, Patti J.; Afonso, Claudio L.

doi:10.1128/jcm.06018-11

Cited by 90 publications

(98 citation statements)

References 27 publications

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“…3,6,29 In contrast, VVNDV-infected chickens usually die or are humanely euthanized by day 5 pi, showing systemic illness without neurologic signs. 3,6,7,9,[27][28][29] When inoculated through the mucosal surfaces (eg, eye drop instillation), the ability of NDV strains to reach the nervous tissue depends heavily on multiple factors, such as peripheral replication, ability to cross the blood-brain barrier and reach the cerebrospinal fluid, and efficiency of replication and spread in neurons and glial cells.…”

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confidence: 99%

Neuropathogenic Capacity of Lentogenic, Mesogenic, and Velogenic Newcastle Disease Virus Strains in Day-Old Chickens

et al. 2015

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Strains of Newcastle disease virus (NDV) have different abilities to elicit neurologic signs. To determine the capacity of different NDV strains to replicate and cause lesions in the brain, independently of their peripheral replication, 1-day-old chickens were inoculated in the subdural space with 7 NDV strains of different virulence (4 velogenic, 2 mesogenic, 1 lentogenic). Velogenic strains induced severe necrotizing and heterophilic ventriculitis and meningitis, as well as edema of the neuroparenchyma, and replicated extensively in the nervous tissue by day 2 postinfection, as demonstrated by immunohistochemistry, when all infected birds died. Clinical signs, microscopic lesions, and viral replication were delayed (days 3 and 4 postinfection) with mesogenic strains. Velogenic and mesogenic NDV strains replicated mainly in neurons, and immunolabeling was first detected in surface-oriented areas (periventricular and submeningeal), possibly as a reflection of the inoculation route. The lentogenic NDV strain did not cause death of infected birds; replication was confined to the epithelium of the ependyma and choroid plexuses; and lesions consisted of lymphoid aggregates limited to the choroid plexuses. Results show that extensive NDV replication in the brain is typical of velogenic and mesogenic, but not lentogenic, NDV strains. In addition, this study suggests that differences in the rate of NDV replication in nervous tissue, not differences in neurotropism, differentiate velogenic from mesogenic NDV strains. This study indicates that intracerebral inoculation might be used as an effective method to study the mechanisms of NDV neuropathogenesis.

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Neuropathogenic Capacity of Lentogenic, Mesogenic, and Velogenic Newcastle Disease Virus Strains in Day-Old Chickens

et al. 2015

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“…RNA isolation and sequencing were performed as previously described (35,36). Briefly, viruses (NDV-DR499-31/08, NDV-DR867-2/08, and NDV-DR28138-4/86) were propagated in eggs, RNA was extracted from allantoic fluids, and F genes were then amplified by reverse transcription-PCR (RT-PCR) and sequenced.…”

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confidence: 99%

“…The complete genome sequence of NDV-DR499-31/08 was determined by using a shotgun RT-PCR/sequencing approach, as previously described (35).…”

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Highly Divergent Virulent Isolates of Newcastle Disease Virus from the Dominican Republic Are Members of a New Genotype That May Have Evolved Unnoticed for Over 2 Decades

Courtney

Susta

Gomez³

et al. 2013

J Clin Microbiol

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“…ND is a major cause of huge economic losses in various parts of the world [5][6]. Newcastle disease virus (NDV) is belong to a group of the avian paramyxovirus -I [7][8][9].…”

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confidence: 99%

Isolation, Identification and Molecular Characterization of Highly Pathogenic Newcastle Disease Virus From Field Outbreaks

Ashraf

Shah

Habib

et al. 2016

Braz. arch. biol. technol.

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Complete Genome and Clinicopathological Characterization of a Virulent Newcastle Disease Virus Isolate from South America

Cited by 90 publications

References 27 publications

Neuropathogenic Capacity of Lentogenic, Mesogenic, and Velogenic Newcastle Disease Virus Strains in Day-Old Chickens

Neuropathogenic Capacity of Lentogenic, Mesogenic, and Velogenic Newcastle Disease Virus Strains in Day-Old Chickens

Highly Divergent Virulent Isolates of Newcastle Disease Virus from the Dominican Republic Are Members of a New Genotype That May Have Evolved Unnoticed for Over 2 Decades

Isolation, Identification and Molecular Characterization of Highly Pathogenic Newcastle Disease Virus From Field Outbreaks

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