Complete genome analysis of Pseudomonas furukawaii ZS1 isolated from grass carp (Ctenopharyngodon idellus) culture water

Abstract

Pseudomonas furukawaii ZS1, isolated from grass carp (Ctenopharyngodon idellus) culture water, exhibits efficient aerobic nitrate reduction without nitrite accumulation; however, the molecular pathway underlying this aerobic nitrate reduction remains unclear. In this study, we constructed a complete genome map of P. furukawaii ZS1 and performed a comparative genomic analysis with a reference strain. The results showed that P. furukawaii ZS1 genome was 6,026,050 bp in size and contained 5,427 predicted protein-coding sequences. The genome contained all the necessary genes for the dissimilatory nitrate reduction to ammonia pathway, but lacked those for the assimilatory nitrate reduction pathway. Simultaneously, the genome also contained genes that converted ammonia to organic nitrogen. The putative genes associated with the nitrogen and oxidative phosphorylation pathways implied that ZS1 can perform respiration and nitrate reduction simultaneously under aerobic conditions. This work provided insights at the molecular level on the nitrogen metabolic pathways in Pseudomonas species.

Microbiota composition and correlations with environmental factors in grass carp (Ctenopharyngodon idella) culture ponds in South China

“…Candidatus Aquiluna, a photoheterotroph ( Kang et al, 2012 ), was reportedly positively correlated with the microbial metabolic activity of organic nitrogen ( Lukwambea et al, 2020 ). Pseudomonas furukawaii ZS1, Acidovorax facilis , Citrobacter diversus , and certain Thauera species also participate in nitrogen removal in aquaculture ponds ( Mai et al, 2021 ; Niu et al, 2022 ). Our results showed that Dechloromonas , Limnohabitans , Candidatus Aquiluna, and Pseudomonas were detected in the pond water and sediment microbiota.…”