Complete genome analysis of a novel iflavirus from a leaf beetle, Aulacophora lewisii

Ye, Zhuang-Xin; Li, Yuehong; Chen, Jianping; Zhang, Chuan‐Xi; Li, Junmin

doi:10.1007/s00705-020-04859-1

Cited by 10 publications

(4 citation statements)

References 12 publications

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“…Although it shows a high degree of similarity with other putative iflaviruses, the phylogenetic analysis clustered this novel virus with other unclassified picorna-like viruses that have previously been reported from insects ( Figure 2 B). In the past few years, several isometric virions have been assigned under the supergroup “picorna-like viruses”, and they have been reported from a wide range of insects including Coleoptera, for example Oryctes rhinoceros (Scarabaeidae) [ 27 ], Aulacophora lewisii (Chrysomelidae) [ 39 ], Harmonia axyridis (Coccinellidae) [ 40 ], and Cheilomenes sexmaculata (Coccinellidae) [ 41 ].…”

Section: Resultsmentioning

confidence: 99%

Transcriptomics Reveal Several Novel Viruses from Canegrubs (Coleoptera: Scarabaeidae) in Central Queensland, Australia

Etebari

Lenancker

Powell

et al. 2022

Viruses

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Canegrubs (Coleoptera: Scarabaeidae) are major pests of sugarcane crops in Australia, but despite long-term and intensive research, no commercially viable biological control agents have been identified. We used the RNA-Seq approach to explore the viriomes of three different species of canegrubs from central Queensland, Australia to identify potential candidates for biological control. We identified six novel RNA viruses, characterized their genomes, and inferred their evolutionary relationships with other closely related viruses. These novel viruses showed similarity to other known members from picornaviruses, benyviruses, sobemoviruses, totiviruses, and reoviruses. The abundance of viral reads varied in these libraries; for example, Dermolepida albohirtum picorna-like virus (9696 nt) was built from 83,894 assembled reads while only 1350 reads mapped to Lepidiota negatoria beny-like virus (6371 nt). Future studies are essential to determine their natural incidence in different life stages of the host, biodiversity, geographical distributions, and potential as biological control agents for these important pests of sugarcane.

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Section: Resultsmentioning

confidence: 99%

Transcriptomics Reveal Several Novel Viruses from Canegrubs (Coleoptera: Scarabaeidae) in Central Queensland, Australia

Etebari

Lenancker

Powell

et al. 2022

Viruses

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“…The most-studied member of the genus Iflavirus , the deformed wing virus (DWV), is the causative agent for honeybee hive collapsing syndrome, which is transmitted by the parasitic mite Varroa destructor [ 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 ] Infection by iflavirus generally occurs orally by feeding on contaminated food, and the virus appears to be distributed in the gut, reproductive organs, fat body, muscle, and central nervous system (CNS) [ 19 ]. Iflaviruses are believed to be arthropod-specific viruses that infect insects in the orders Lepidoptera, Hymenoptera, Hemiptera, Diptera, Coleoptera, and Orthoptera [ 9 , 19 , 20 , 21 , 22 , 23 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Iflavirus in the Red Flour Beetle, Tribolium castaneum (Coleoptera; Tenebrionidae)

Fatehi

Aikins

Philips

et al. 2023

Insects

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Iflavirus is a group of viruses distributed mainly in arthropod species. We surveyed Tribolium castaneum iflavirus (TcIV) in different laboratory strains and in Sequence Read Archives (SRA) in GenBank. TcIV is highly specific to only T. castaneum and is not found in seven other Tenebrionid species, including the closely related species T. freemani. The same strains from different laboratories and different strains displayed largely different degrees of infections in the examination of 50 different lines by using Taqman-based quantitative PCR. We found that ~63% (27 out of 43 strains) of T. castaneum strains in different laboratories are positive for TcIV PCR with large degrees of variation, in the range of seven orders of magnitude, indicating that the TcIV is highly fluctuating depending on the rearing conditions. The TcIV was prevalent in the nervous system with low levels found in the gonad and gut. The transovarial transmission was supported in the experiment with surface-sterilized eggs. Interestingly, TcIV infection did not show observable pathogenicity. TcIV offers an opportunity to study the interaction between the virus and the immune system of this model beetle species.

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“…(Culex picorna-like virus) [8]. For Coleoptera, several picorna-like viruses has been reported from different host insect families, such as Scarabaeidae (oryctes rhinoceros) [9], Chrysomelidae (Aulacophora lewisii) [10] and Coccinellidae (Harmonia axyridis) [11]. Cheilomenes sexmaculata, another member of the family Coccinellidae, is an important aphidophagous predator for the biocontrol of agricultural pests, which is widely distributed in Asia [12].…”

Section: Introductionmentioning

confidence: 99%

Complete genome analysis of a novel picorna-like virus from a ladybird beetle (Cheilomenes sexmaculata)

Chen

et al. 2022

Arch Virol

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The ladybird beetle Cheilomenes sexmaculata (family Coccinellidae, order Coleoptera), is a common insect predator of agricultural pests. In this study, the full genome sequence of a novel picorna-like virus, temporarily named "Cheilomenes sexmaculata picorna-like virus 1" (CSPLV1), was identi ed from C. sexmaculata. The full-length sequence of CSPLV1 was 11,384 nucleotide (nt) in length (excluding the polyA tail) with one predicted open reading frame (ORF) encoding 3727 amino acids, a 13 nt 5' untranslated region (UTR) and a 187 nt 3' UTR. The ORF of CSPLV1 consisted of four distinct domains including an RNA virus helicase domain (3029-3319 nt), a peptidase domain (5555-6121 nt), an RNA-dependent RNA polymerase domain (7154-8101 nt) and a picorna-like coat protein domain (8606-9283nt). Phylogenetic analysis based on the conserved RdRP sequence showed that CSPLV1, together with Wuhan house centipede virus 3, Hypera postica associated virus 1 and Diabrotica undecimpunctata virus 1, formed as an unclassi ed group which is closely related to the clade Solinviviridae. To the best of our knowledge, CSPLV1 is the rst picorna-like virus revealed in C. sexmaculata.

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Complete genome analysis of a novel iflavirus from a leaf beetle, Aulacophora lewisii

Cited by 10 publications

References 12 publications

Transcriptomics Reveal Several Novel Viruses from Canegrubs (Coleoptera: Scarabaeidae) in Central Queensland, Australia

Transcriptomics Reveal Several Novel Viruses from Canegrubs (Coleoptera: Scarabaeidae) in Central Queensland, Australia

Characterization of Iflavirus in the Red Flour Beetle, Tribolium castaneum (Coleoptera; Tenebrionidae)

Complete genome analysis of a novel picorna-like virus from a ladybird beetle (Cheilomenes sexmaculata)

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