Complete extraction in a form suitable for liquid scintillation counting of tritium-labeled proteins from polyacrylamide gels

Species Distribution of Paraoxon‐Resistant Brain Polypeptides Radiolabeled with Diisopropyl Phosphorofluoridate ([3H]DiPF): Electrophoretic Assay for the Aged Polypeptide of [3H]DiPF‐Labelled Neuropathy Target Esterase

“…Williams ( 1983) limited his use of the "volatilisable count" assay to soluble fractions obtained by SDS-gel filtration, because extraction of tritium-labelled proteins from polyacrylamide gels was only -60% efficient. However, slab PAGE enables several samples to be separated quickly with good resolution, and we have recently developed a technique for the complete recovery of labelled proteins from polyacrylamide gels cross-linked with N,N'-diallyl tartardiamide (DATD) (Meredith and Johnson, 1987), in which the conventional periodate digest is supplemented with an alkaline quaternary ammonium reagent. This procedure is particularly convenient in monitoring [ 3H]DiPF-labelled NTE, because not only can labelled polypeptides be completely extracted from gel slices, but also alkaline hydrolyses can be performed in situ using the same quaternary ammonium base.…”