Complete DNA sequence of lymphotropic papovavirus: Prototype of a new species of the polyomavirus genus

Pawlita, Michael; Clad, Andreas; Hausen, Harald zur

doi:10.1016/0042-6822(85)90108-4

Cited by 77 publications

(49 citation statements)

References 52 publications

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“…However, when 10 393 Figure 2A and B, the sequence 5'-ATGCAAA-3' homologous to the immunoglobulin heavy chain enhancer (position 212 -206) is underlined, and the polyoma virus enhancer homology (P-motif, position 196-186) is boxed. References for the various sequence elements are as follows (see also text): LPV enhancer (Pawlita et al, 1985;Mosthaf et al, 1985); BK virus (BKV) enhancer (Rosenthal et al, 1983); Igx light chain enhancer (Picard and Schaffner, 1984;Queen and Stafford, 1984); consensus sequences of the upstream promoter elements of the immunoglobulin heavy (VH) and light (VL, opposite strand) chain genes (Falkner and Zachau, 1984;Parslow et al, 1984); Ig heavy chain enhancer (opposite strand, Ephrussi et al, 1985 and references therein; the star indicates the C complementary to the G protected against dimethylsulfate modification, see text); Xenopus Ul/U2 RNA genes (Mattaj et al, 1985;Ciiberto et al, 1985;Krol et al, 1985); polyoma virus enhancer (opposite strand, Veldman et al, 1985;Ruley and Fried, 1983). For comparison the ElA-like motif of the polyoma virus enhancer (Hearing and Shenk, 1983;Herbomel et al 1984) is shown.…”

Section: Resultsmentioning

confidence: 99%

“…Figure 2A and data not shown). Sequence elements closely related to the Sph-motifs are found as a single motif in the BK virus (Rosenthal et al, 1983) and as a repeat in the lymphotropic papovavirus (LPV) (Furuno et al, 1984;Pawlita et al, 1985;Mosthaf et al, 1985) (see Figure 6), where their function has not yet been studied. The Sph-motif is also present in the immunoglobulin x-light chain enhancer (Picard and Schaffner, 1984;Queen and Baltimore, 1983), where its deletion is detrimental to enhancer activity (Queen and Stafford, 1984).…”

Section: Discussion 7he Sv40 Enhancer Domainsmentioning

confidence: 99%

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Multiple sequence motifs are involved in SV40 enhancer function.

Zenke¹,

Grundström²,

Matthes³

et al. 1986

The EMBO Journal

493

322

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A systematic mutagenesis of the SV40 enhancer indicates that it spans -100 bp and is composed of at least two distinct DNA domains which exhibit very little enhancing activity on their own. Their association results in a 400-fold enhancement of transcription, virtually irrespective of their relative orientation and, to some extent, of the distance between them. Enhancer activity can also be generated by duplication of either domain. We show also that the activity of each domain is due to the presence of several specific sequence motifs. These motifs are found assorted in different combinations in other viral and cellular enhancers. Key words: transcription/site-directed mutagenesis/promoter/ RNA polymerase B(ll)/simian virus 40 1983;Lusky et al., 1983;Hearing and Shenk, 1983;Veldman et al., 1985). However, such enhancer consensus sequences occur in DNA segments without enhancer properties, and enhancer activity can be generated by duplicating DNA sequences without enhancer activity on their own (Weber et al., 1984;Swimmer and Shenk, 1984), thus questioning the real functional significance of these consensus sequences.Therefore, we decided to determine, at the nucleotide level, the DNA sequences essential for the activity of the prototype SV40 enhancer. Systematic deletions and point mutations have been constructed throughout the enhancer region and their effect on SV40 early transcription investigated in vivo, using a transient expression assay in HeLa cells. We show here that the SV40 enhancer encompasses a large DNA segment of -100 nucleotides containing the 72-bp sequence, but also extending further upstream. Furthermore, the present study reveals that the enhancer is composed of at least two distinct DNA domains which exhibit very little enhancing activity on their own. However, their association results in a dramatic 400-fold enhancement of transcription, virtually irrespective of their relative orientation and, to some extent, of the distance between them. Enhancer activity can also be generated by duplication of either domain. In addition, we show that the activity of each domain is due to the presence of several specific sequence motifs. Various assortments of these motifs are found in other viral and cellular enhancers, suggesting that enhancers are mosaics of a limited number of basic evolutionary related sequence motifs.

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Section: Resultsmentioning

confidence: 99%

Section: Discussion 7he Sv40 Enhancer Domainsmentioning

confidence: 99%

Multiple sequence motifs are involved in SV40 enhancer function.

Zenke¹,

Grundström²,

Matthes³

et al. 1986

The EMBO Journal

493

322

View full text Add to dashboard Cite

show abstract

“…The genome sequences of finch polyomavirus (FPyV) and crow polyomavirus (CPyV) were reassembled from the sequence fragments using the EditSeq module of the DNASTAR software package (Lasergene, Madison, WI). For sequence alignment and phylogenetic analyses, the genome sequences of 10 polyomaviruses were derived from the GenBank database (under the given accession numbers): APV, strain BFDV-1 (AF241168) (49); BKPyV (NC001538) (47); bovine polyomavirus (D13942) (46); GHPV (AY140894) (17); hamster polyomavirus ([HaPyV] M26281) (7); JCPyV (NC001699) (8); ␤-lymphotropic polyomavirus, strain K38 (K02562) (36); murine polyomavirus (MPyV), strain Crawford small-plaque (K02737) (42); murine pneumotropic virus (MPtV), strain Kilham (M57473) (29); and SV40, strain K661 (AF038616) (23). Alignment was performed using the MegAlign module of the above-mentioned software package with the ClustalW method (50) and the "residue identity" weight table.…”

Section: Methodsmentioning

confidence: 99%

Characterization of Two Novel Polyomaviruses of Birds by Using Multiply Primed Rolling-Circle Amplification of Their Genomes

Johne

Wittig²,

Fernández-de-Luco

et al. 2006

J Virol

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“…In this report we present the nucleotide sequence of the complete BPyV genome, which is compared to the sequences of the genomes of SV40 (Fiers et al, 1978) and mouse polyomavirus (PyV; Griffin et al, 1981). In some relevant instances the known sequences of human polyomaviruses BK-Dunlop strain (BKV-dun;Seif et al, 1979) and JC virus (JCV; Frisque et al, 1984), and other animal polyomaviruses, monkey B-lymphotropic polyomavirus (LPV;Pawlita et al, 1985), hamster polyomavirus (HaPV; Delmas et al, 1985) and the budgerigar fledgling disease virus (BFDV; Rott et al, 1988) are also included in the comparison.…”

Section: Introductionmentioning

confidence: 99%

The Complete Nucleotide Sequence of Bovine Polyomavirus

Schuurman

Sol

Noordaa

1990

Journal of General Virology

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The complete sequence of the genome of bovine polyomavirus (BPyV), formerly known as the CK isolate of the stump-tailed macaque virus, is presented. The genomic organization of BPyV is similar to that of the non-rodent polyomaviruses. With a genome size of 4697 bp, BPyV has the smallest polyomavirus genome known so far. When compared to simian virus 40 (SV40), the shortness of the BPyV genome is due mainly to differences in the coding capacity of the BPyV early region. The first exon of the proposed large T antigen encodes only 35 amino acids; also, a coding region corresponding to the C-terminal 64 amino acids of the SV40 large T antigen is absent in BPyV. It is proposed that the nucleotide sequence encompassing the small t antigen coding sequence contains an intron sequence of 71 nucleotides. Together the two exon sequences encode a 124 amino acid protein. We conclude that this may be the first example of a polyomavirus that has a small t antigen which is translated from two exon sequences. The enhancer region of BPyV does not show homology to the SV40 enhancer sequences. An agnogene is present with a coding capacity of 118 amino acid residues. The highest degree of homology to SV40 and PyV is present in the VP1 molecule.

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Complete DNA sequence of lymphotropic papovavirus: Prototype of a new species of the polyomavirus genus

Cited by 77 publications

References 52 publications

Multiple sequence motifs are involved in SV40 enhancer function.

Multiple sequence motifs are involved in SV40 enhancer function.

Characterization of Two Novel Polyomaviruses of Birds by Using Multiply Primed Rolling-Circle Amplification of Their Genomes

The Complete Nucleotide Sequence of Bovine Polyomavirus

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