Complete discrimination of docosahexaenoate from arachidonate by 85 kDa cytosolic phospholipase A2 during the hydrolysis of diacyl- and alkenylacylglycerophosphoethanolamine

Omega-3 fatty acids cause dramatic changes in TLR4 and purinergic eicosanoid signaling

“…With P2X 7 stimulation, cPLA 2 is translocated to the membrane in a Ca 2+ -dependent manor, where AA and EPA appear to be equally good substrates, and DHA is essentially not released after activation (29,30). Our results with purinergic stimulation were consistent with these observations, although DHA release was significant when dramatically increased in phospholipids, as was DPA.…”

Metabolism and phospholipid assembly of polyunsaturated fatty acids in human bone marrow mesenchymal stromal cells

“…The cPLA 2 IV preferentially liberates 20:4n-6 from the sn-2 position and favors PC and PI as substrates, but also accepts PE with lower rates of hydrolysis (63). In human eosinophilic leukemia cells, the activity of cPLA 2 toward PE species with 20:5n-3 and 22:6n-3 was found to be much lower than toward the 20:4n-6-containing PE (64). Other PLA 2 forms have different GPL class preferences and also use PE and PEp species as the PUFA donor (65).…”

Extracellular-derived calcium does not initiate in vivo neurotransmission involving docosahexaenoic acid

“…cPLA 2 ( ␣ and ␤ ) requires a low concentration of Ca 2+ for its translocation to the membrane and activation (0.3-1 M) and is selective for AA during acute stimulation of cells by diverse agents in vitro ( 8,9 ). iPLA 2 does not require Ca 2+ for activation (10)(11)(12) and is selective for DHA in isolated glial cells and in the test tube ( 13 ).…”