Complete Coding Sequence of a Lumpy Skin Disease Virus Strain Isolated during the 2016 Outbreak in Kazakhstan

Mathijs, Élisabeth; Vandenbussche, Frank; Saduakassova, Meruyert A.; Kabduldanov, Tursyn; Haegeman, Andy; Aerts, Laetitia; Kyzaibayev, Taskyn; Sultanov, Akhmetzhan A.; Borm, Steven Van; Clercq, Kris De

doi:10.1128/mra.01399-19

Cited by 12 publications

(11 citation statements)

References 7 publications

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“…In this experiment, LSDV isolate Kubash/KAZ/16 recovered from an infected cow during an outbreak of LSD in Atyrau, Kazakhstan in 2016 were used [ 12 ]. Virus was grown on primary lamb testis (LT) and with three in vivo passages through calves.…”

Section: Methodsmentioning

confidence: 99%

Retention of lumpy skin disease virus in Stomoxys spp (Stomoxys calcitrans, Stomoxys sitiens, Stomoxys indica) following intrathoracic inoculation, Diptera: Muscidae

et al. 2021

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Lumpy skin disease (LSD) is an emerging disease of cattle in Kazakhstan and the means of transmission remains uncertain. In the current study, retention of Lumpy Skin Disease Virus (LSDV) by three Stomoxys species following intrathoracic inoculation was demonstrated under laboratory conditions. A virulent LSDV strain was injected into the thorax of flies to bypass the midgut barrier. The fate of the pathogen in the hemolymph of the flies was examined using PCR and virus isolation tests. LSDV was isolated from all three Stomoxys species up to 24h post inoculation while virus DNA was detectable up to 7d post inoculation.

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Section: Methodsmentioning

confidence: 99%

Retention of lumpy skin disease virus in Stomoxys spp (Stomoxys calcitrans, Stomoxys sitiens, Stomoxys indica) following intrathoracic inoculation, Diptera: Muscidae

et al. 2021

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“…Kazakh Scientific-Research Veterinary Institute LLP (Kazakhstan) and Sciensano, Unit Exotic Viruses and Particular Diseases (Belgium) and has been deposited in GenBank under accession number MN642592 (LSDV isolate Kubash/KAZ/16) (Mathijs et al 2020).…”

Section: Complete Genome Sequencing Of Lsdv Field Strain Was Performementioning

confidence: 99%

Emergence of the First Lumpy Skin Disease Outbreak Among Livestock in the Republic of Kazakhstan in 2016.

Issimov¹,

Rametov²,

Zhugunissov³

et al. 2020

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Lumpy skin disease (LSD) is an emerging transboundary viral disease of cattle originating from the African continent. Here we describe the first LSD outbreak reported in the Republic of Kazakhstan, in July 2016. Initially, LSD was reported in a cattle farm located 49 km from Kazakh –Russian border in, Atyrau Oblast in West Kazakhstan. Subsequently, the disease spread to neighbouring farms situated within the same district. Following a preliminary investigation, the local State Veterinary Service declared a strict quarantine according to the State Contingency Plan, along with immediate total stamping out and cattle movement restrictions. During the outbreak, the number of affected cattle within an epidemiological unit reached 459 cattle out of registered 3557 susceptible cattle with 12.90% morbidity and 0.96%, mortality. This manuscript presents the epidemiological situation, the diagnosis, the control measures including mass vaccination and the stamping out campaign.s

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“…In fact, the Republic of Kazakhstan is closely located (the minimum distance to the Southeast border is 98.64 km) to the investigated Nesterovo Village of Saratov Region and some other Russian regions, such as: Volgograd, Samara, Orenburg, Kurgan, Tyumen and Omsk (Figures 2 and 3). However, only a single LSDV strain was detected in Saratov Region in 2017, belonging to the same Type Ia that was identified in the Republic of Kazakhstan in 2016 [44].…”

Section: Phylogeographic Analysismentioning

confidence: 83%

“…There is total annual vaccination of all cattle in the Russian regions with a marked level of LSD morbidity, especially if the territory borders with countries in which LSD cases were reported or LSDV was detected either in the past or at present. One of such regions is Saratov Region, which borders the Republic of Kazakhstan where the LSDV field strains were reported in 2016 [44]. In fact, in Saratov Region at least 30 LSD outbreaks were officially registered during 2017-2019 [45].…”

Section: Introductionmentioning

confidence: 99%

Genetic Evidence of Multiple Introductions of Lumpy Skin Disease Virus into Saratov Region, Russia

et al. 2021

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Lumpy skin disease virus (LSDV) is the causative agent of lumpy skin disease (LSD) that has been recently reported in the South-East and North Asian parts of the Russian Federation. During 2017–2019, there were more than 30 LSD outbreaks in Saratov Region despite active inoculation of cattle with heterologous vaccine. Importantly, the first case of the novel recombinant LSDV strain was reported here in 2017. This study aimed to determine the main clonal lineage(s) of LSDV strains circulated within Saratov Region and other regions of Russia since the first introduction of LSDV. The molecular typing and subtyping based on the coding regions of the G-protein-coupled chemokine receptor (GPCR) gene resulted in a discrimination of all outbreak-related LSDV strains into two main types, such as Type I and Type II, and subtypes Ia-d and IIa-g. Phylogenetically, eleven LSDV lineages were revealed in Russia including the five ones in Saratov Region. They were the following: (i) the Neethling wild Type Ia/2017; (ii) the recombinant Saratov IIc/2017/2019; (iii) the specific Dergachevskyi IId/2017; (iv) the Khvalynsky IIg/2018, and (v) the Haden-Type IIa lineage for the six LSDV strains detected in cattle immunized with heterologous vaccine during the last LSD outbreak in the Saratov Region, Nesterovo Village, in 2019 (Nesterovo-2019 strains). A single LSDV strain detected in Saratov Region in 2017 had the same Type Ia that was identified in 2016 in the bordered Republic of Kazakhstan. Phylogeographic analysis demonstrated three nominal clusters of LSDV types in the following Russian Federation territories: (I) the Central European part; (II) the South-East of the European part; (III) the North Asian part. Cluster I was represented by mainly Type I strains, while both Clusters 2 and 3 contained predominantly Type II strains. The Clusters I and II partially overlapped, while Cluster 3 was separate. Multiple introductions of LSDV into Saratov Region in 2017–2019 using GPCR-based molecular typing and subtyping were revealed. This scheme is a promising tool for molecular discrimination of LSDV strains derived from both vaccinated and unvaccinated against LSD cattle as well as for molecular epidemiology.

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Complete Coding Sequence of a Lumpy Skin Disease Virus Strain Isolated during the 2016 Outbreak in Kazakhstan

Abstract: Lumpy skin disease virus (LSDV) causes an economically important disease in cattle. Here, we report the complete coding sequence of the LSDV isolate Kubash/KAZ/16, detected in a clinical sample from an infected cow from the outbreak reported on 7 July 2016 in Kazakhstan (Atyrau Region).

Cited by 12 publications

References 7 publications

Retention of lumpy skin disease virus in Stomoxys spp (Stomoxys calcitrans, Stomoxys sitiens, Stomoxys indica) following intrathoracic inoculation, Diptera: Muscidae

Retention of lumpy skin disease virus in Stomoxys spp (Stomoxys calcitrans, Stomoxys sitiens, Stomoxys indica) following intrathoracic inoculation, Diptera: Muscidae

Emergence of the First Lumpy Skin Disease Outbreak Among Livestock in the Republic of Kazakhstan in 2016.

Genetic Evidence of Multiple Introductions of Lumpy Skin Disease Virus into Saratov Region, Russia

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