Complete coding region of the new HLA‐DQB1*0612 allele, obtained by RT‐PCR

Abstract

A novel HLA-DQB1 allele was detected by oligotyping in the Bubi population of Equatorial Guinea. In order to characterize the new allelic variant, a RT-PCR method which permitted the cloning of its complete coding region was designed. With this method, we have determined the nucleotide sequence of the new DQB1*0612 allele, related to *0604 and *0609 but differing from them at polymorphic codon 70. A proposal for the improvement of the sequencing strategies of HLA class II alleles is made.

HLA class I and class II allele distribution in the Bubi population from the island of Bioko (Equatorial Guinea)

“…DQBl*02, *0301, *03032, *0501 and *0602 are present in more than 20% of the sample studied. Sequencing of the DQBl genes of a cell showing an uncommon PCR-SSO hybridization pattern resulted in the finding of a new allele, DQB1*0612 (25), showing great similarity with DQBl*O6O4 and *0609. This new allele was found in apparent association with DRBl*1301, DRB3*0202 and DQA 1 *O 103.…”

Nomenclature for Factors of the HLA System, 1996

“…-DQB1*06GB GB002 X96420 (Vilches et al, 1996b) This sequence can only be assigned a four digit allele name, as it is either published only as a protein or partial nucleotide sequence. This reference is to a confirmatory sequence.…”

High‐resolution HLA‐DQB1 typing using the polymerase chain reaction and sequence‐specific primers

“…However, since initial reports of DQBl typing by PCR-SSP (8)(9)(10)(11)(12), there has been a prodigious expansion of recognized alleles, not all of which can be discriminated by existing methods. In particular, seven DQB1*03 and 13 DQB1*06 alleles have now been described (1, [13][14][15][16][17]. We have improved the methods described by Olerup et al (9), and Bunce et al (8,18), so that all recognized alleles, except for synonymous variants, may be distinguished.…”