Complete chloroplast genome sequence ofThalictrum viscosumW.T.Wang & S.H.Wang, 1979 (Ranunculaceae)

Abstract

Thalictrum viscosum W.T.Wang & S.H.Wang, 1979 is a flowering plant species in family Ranunculaceae that is endemic to Yunnan province of China. To facilitate genetic study of T. viscosum , we de novo assembled and annotated the complete chloroplast (cp) genome of T. viscosum for the first time. The total length of the cp genome of T. viscosum was 155,984 bp, with a GC content of 38.4%. The T. viscosum cp genome had a typical quadripartite structure with a large single-copy region of 85,339 bp, a small single-copy region of 17,656 bp, and a pair of inverted repeat regions of 26,495 bp. The cp genome consisted of 133 genes, including 87 protein-coding genes, 38 transfer RNA genes, and eight ribosomal RNA genes. We performed phylogenetic analysis of T. viscosum with the maximum-likelihood phylogenetic tree and indicated that T. viscosum was closely related to T. cirrhosum and T. foeniculaceum.

The first complete chloroplast genome of Thalictrum fargesii: insights into phylogeny and species identification

“…Overall, the structure and organization of the nine observed cp genomes in Thalictrum , including T. fargesii , follow the typical structure commonly found in angiosperms, except for a difference in tRNA content in T. viscosum , which contains 38 tRNAs instead of 37 ( Park et al., 2015 ; Daniell et al., 2016 ; He et al., 2019 ; Morales-Briones et al., 2019 ; He et al., 2021 ; Cai et al., 2022 ; Zhe et al., 2023 ). The total length, GC content, and gene composition were almost identical in the cp genomes of all studied species, and these highly conserved features might be due to a typical evolutionary passage in Thalictrum ( Khan et al., 2020 ; Villanueva-Corrales et al., 2021 ; Feng et al., 2022 ).…”

“…Next-generation sequencing was used to sequence the cp genomes of the ethnodrug species T. fargesii . The plastome of this species varies in terms of genomic structure, nucleotide diversity, structural alterations, and phylogeny with the plastomes of Thalictrum species ( Park et al., 2015 ; He et al., 2019 ; Morales-Briones et al., 2019 ; He et al., 2021 ; Cai et al., 2022 ; Zhe et al., 2023 ). For instance, there is an apparent difference between Thalictrum plastomes in the IR-SC boundary areas, which causes variations in genome size ( Xiang et al., 2022 ).…”

“…(2022) found that 116 IGS regions had stronger support than 114 gene sequences and revealed that the closest phylogenetic relationship existed within T. minus and T. aquilegiifolium , and T. coreanum and T. thalictroides . Several studies have uncovered that close relationships were present between T. minus and T. tenue ( He et al., 2019 ; Lin et al., 2021 ; Pu et al., 2022 ), T. tenue and T. petaloideum ( He et al., 2019 ; Pu et al., 2022 ), T. minus and T. petaloideum ( He et al., 2019 ; Pu et al., 2022 ), T. minus and T. aquilegiifolium ( Cai et al., 2022 ; Michimoto et al., 2022 ; Xiang et al., 2022 ), T. viscosum and T. cirrhosum ( Cai et al., 2022 ; Michimoto et al., 2022 ), and T. coreanum and T. thalictroides ( He et al., 2021 ; Lin et al., 2021 ; Michimoto et al., 2022 ; Xiang et al., 2022 ) based on all sequences and whole cp genome. However, none of their circumscribed relationships have uncovered the T. fargesii status within this genus.…”

“…According to their results, T. fargesii is closely related to T. tenue and T. petaloideum , supporting the conclusion of an earlier study that T. baicalense is closely related to T. tenue, T. minus , and T. petaloideum ( He et al., 2021 ). Interestingly, T. viscosum has been reported to be closely related to T. cirrhosum and T. foeniculaceum ( Cai et al., 2022 ). Conferring with more Thalictrum taxon samples, our phylogenetic studies of cp genome sequences, reported for the first time, revealed significantly enriched phylogenetic resolution and provided robust inferences of intraspecific relationships.…”

“…Identifying member species within the Ranunculaceae family, especially within the Thalictrum, is taxonomically challenging because of the inability to distinguish these species through universal molecular markers and similar morphological traits (Li et al, 2020). Previous studies have revealed that Thalictrum species form a highly supported clade, indicating that they are monophyletic (Cai et al, 2022;Michimoto et al, 2022). Thalictrum species exhibit small chromosomes compared with those of Rununculus, and this genus is challenging and diverse in terms of taxonomy and phylogeny based on variations in morphological traits (Langlet, 1927;Tamura, 1995;Xiang et al, 2022).…”