Complete chloroplast genome of Prunus pensylvanica and its implications for the phylogenetic position within Prunus sensu lato (Rosaceae)

Abstract

Prunus pensylvanica is one of the two native cherry species of North America. We determined the first complete chloroplast genome of P. pensylvanica using genome-skimming approach. The cp genome was 157,953 bp long, with a large single-copy region (LSC) of 86,030 bp and a small single-copy region (SSC) of 19,135 bp separated by a pair of inverted repeats (IRs) of 26,394 bp. It encodes 129 genes, including 84 protein-coding genes, 37 tRNA genes, and 8 ribosomal RNA genes. We also reconstructed the phylogeny of Prunus sensu lato using maximum likelihood (ML) method, including our data and previously reported cp genomes of related taxa. The phylogenetic analysis indicated that P. pensylvanica is closely related to P. emarginata. ARTICLE HISTORY

Comparative chloroplast genomes of Prunus subgenus Cerasus (Rosaceae): insights into sequence variations and phylogenetic relationships

“…We also downloaded 22 available cp genomes of Prunus, comprising P. avium (Zhao et al 2019a), P. campanulata, P. emarginata (Chen et al 2019a), P. cerasoides (Xu et al 2018), P. conradinae (Yan et al 2020), P. dictyoneura (Liu et al 2019), P. dielsiana (Zhao et al 2019b), P. discoidea (Li et al 2019), P. canescens (Chen et al 2019b), P. jamasakura (Yi et al 2020), P. matuurae (Sun and Liu. 2019), P. kumanoensis (Sun et al 2019a), P. maximowiczii, P. pensylvanica (Zhang et al 2019), P. pseudocerasus (Feng et al 2018), P. rufa (Li et al 2020b), P. serrulata, P. spachiana (Sheng et al 2020), P. speciosa (Sun et al 2019b), P. subhirtella, P. takesimensis (Cho et al 2018), and P. yedoensis (Cho et al 2015), then used for subsequent comparative analyses. These cp genome sequences were obtained from the Gen-Bank database (https:// www.…”