Complete chloroplast genome of a subtropical fruit tree Macadamia ternifolia (Proteaceae)

Abstract

Macadamia ternifolia is a subtropical fruit tree of the family Proteaceae. Chloroplast genome sequences play a significant role in the development of molecular markers in plant phylogenetic and population genetic studies. In this study, we report the complete chloroplast genome sequence of M. ternifolia. The chloroplast genome is 159,669 bp long and includes 113 genes. Its LSC, SSC, and IR regions are 88,072, 18,801, and 26,408 bp long, respectively. Sequence comparison of M. ternifolia and M. integrifolia indicates large sequence conservation between these two species, only few sequence variations including single nucleotide polymorphisms (SNPs) and inserts/deletes (InDels) were detected. ARTICLE HISTORY

The whole chloroplast genome sequence of Macadamia tetraphylla (Proteaceae)

“… 2014 ) and M. ternifolia (Liu et al. 2017 ), and other species were highly supported by a bootstrap value of 100 ( Figure 1 ).…”

“…Genetic analysis can determine the potential long-term viability of this species. Only two chloroplast genomes were reported in Proteaceae, the M. integrifoia (Nock et al 2014) and M. ternifolia (Liu et al 2017). In the study, we report the complete chloroplast genome of M. tetraphylla, and hope it will help restore the macadamia species to prevent the extinction of this single wild population.…”

“…Phylogenetic tree included M. tetraphylla, M. integrifoia and the other 10 representative early diverging eudicotyledons species was constructed. Maximumlikelihood (ML) analysis exhibited that M. tetraphylla clustered with M. integrifoia (Nock et al 2014) and M. ternifolia (Liu et al 2017), and other species were highly supported by a bootstrap value of 100 ( Figure 1).…”

“… 2014 ) and M. ternifolia (Liu et al. 2017 ). In the study, we report the complete chloroplast genome of M. tetraphylla , and hope it will help restore the macadamia species to prevent the extinction of this single wild population.…”

Genome assembly and annotation ofMacadamia tetraphylla

“…Mb to 780 Mb [17][18][19] , but research on the genome of macadamia was very few. In 2014, 2017 and 2018, the chloroplast genome of M. integrifolia, M. ternifolia and M. tetraphylla have been sequenced by Australian and Chinese researchers [20][21][22] , and in 2016, the darft genome and transcriptome of M. integrifolia cultivar 741 have been sequenced, a total length of 518 Mb have been assembly, which spans approximately 79% of the 3 estimated genome size 18 . But up to now, the genome sequencing of M. tetraphylla has not yet been reported.…”