Complete characterization of new isolates of Neptunomonas phycophila leads to emend its description and opens possibilities of biotechnological applications

Abstract

Five strains were isolated from gonad of Great scallop (Pecten maximus) broodstock in a Norwegian hatchery. The study of 16S rRNA gene sequences showed that these isolates belong to Neptunomonas phycophila, a bacterium originally isolated from a symbiont of the anemone Aiptasia tagetes from Puerto Rico. The gyrB and rpoB genes sequences confirmed the affiliation of the scallop isolates to this species. Phenotypic characterization was performed and some differences between the Norwegian isolates and the type strain of N. phycophila were detected, such as ranges of temperature, pH, and tolerance to salinity or the use of several substrates as sole carbon source which lead to an emended description of the species. The strain 3CM2.5 showed phosphatidylethanolamine and phosphatidylglycerol as the major polar lipids. The whole genomes of the scallop strain 3CM2.5 and type strain of the species CECT 8716T were obtained and the annotation of these genomes revealed the presence of genes involved in degradation of aromatic compounds in both strains. Results obtained not only widen the geographical and host ranges of N. phycophila, but also point out possible biotechnological applications for this bacterial species.

Bioremediation of Petroleum Hydrocarbons in Seawater: Prospects of Using Lyophilized Native Hydrocarbon-Degrading Bacteria

“…[59], Alteromonas sp. [60], Neptunomonas phycophila [61] and the Alphaproteobacteria Sulfitobacter sp. [13].…”

“…Contrary to this previous study, in the present work, the microcosm flasks were kept closed. The lower oxygen input to the experiment may explain the lower THPs removal since oxygen promotes the action of oxygenases, strong aliphatic hydrocarbons hydrocarbon-degrading enzymes [17,63], and isolation of the facultative anaerobe Neptunomonas phycophila [61] species. Nonetheless, the introduction of the lyophilized consortium accelerated the degradation of petroleum, a feature observed in some other studies.…”

The Prokaryotic and Eukaryotic Microbiome of Pacific Oyster Spat is Shaped by Ocean Warming but not Acidification

“…4c-d), which could be because of different temperature optima among taxa. For example, prokaryote genera that were more abundant at 24°C, included Methylotenera (Kalyuzhnaya et al 2006), Neptunomonas (Diéguez et al 2017) and Methylophaga (Villeneuve et al 2013), genera that comprised some species with optimum growth temperatures ranging from 23 to 30°C (Lyubetsky et al 2020). In contrast, prokaryotic genera that were abundant at the 18°C treatment included Arcobacter (Van Driessche and Houf 2008), Colwellia (Techtmann et al 2016), and Saprospira (Saw et al 2012); these genera contain members that grow well at 18°C, and can tolerate temperatures as low as 4°C.…”

DNA-foraging bacteria in the seafloor

“…Together, this suggested that degradation of extracellular DNA is a specialised physiological feature of only some Nitrincolaceae members. Indeed, various members of Nitrincolaceae are known to be a relatively versatile in catabolic terms, whereby they are known to degrade various carbohydrates, alcohols, organic acids and complex polycyclic aromatic hydrocarbons 45 . Alternatively, the low abundance Nitrincolaceae detected in this study and that could not be sampled for genomically, may have distinct genomes that harbour more expansive gene complements for enabling DNA catabolism.…”