‘Complete blood counts and automated leucocyte differential results obtained by Siemens <scp>ADVIA</scp> 2120i in 145 cases of acute leukaemia in adults: insights into cellular pathophysiology and differential diagnosis’

Canovi, Simone; Campioli, Daniele

doi:10.1111/ijlh.12547

Cited by 2 publications

(2 citation statements)

References 9 publications

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“…To identify the values of blast suspect, LUC, DN II, and DN Iessential for the prediction of leukemia relapse, we determined the odds ratio (OR) by logistic regression analysis ( and blasts ≥28.5% [10].…”

Section: Resultsmentioning

confidence: 99%

Large Unstained Cell, Blast Suspect and Delta Neutrophil Index ll Analyzed with Automated Hematology Analyzer as Parameters for the Prediction of Acute Leukemia Relapse

2019

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of acute leukemia after HSCT poses a great risk to the patients and is often associated with poor prognosis [2, 3]. Automated hematology analyzers evaluate multiple morphological and quantitative parameters such as nucleated red blood cells (NRBCs), large platelets, and left shift in addition to routine cytograms. ADVIA 2120i (Siemens Healthcare Diagnostics, Deer eld, IL., USA) provides multiple morphology ags such as large unstained cell (LUC), blast suspect, and immature granulocytes to assist in the identication of abnormal samples. LUC is de ned as a large peroxidasenegative cell that may not be classi ed as a reactive lymphocyte, blast, or other cell type [4]. Blast suspect is a cell with dispersed chromatin as per the basophil/lobularity method of ADVIA 2120i (Siemens Healthcare Diagnostics). Delta neutrophil index (DN l) is a value obtained from the automatic calculation of immature INTRODUCTION The prognosis of acute leukemia has signi cantly improved since the inclusion of hematopoietic stem cell transplantation

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Section: Resultsmentioning

confidence: 99%

Large Unstained Cell, Blast Suspect and Delta Neutrophil Index ll Analyzed with Automated Hematology Analyzer as Parameters for the Prediction of Acute Leukemia Relapse

2019

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“…Furthermore, it is important to consider that an automatic haematology analysis system, such as ADVIA 2120i, distinguishes monocytes and neutrophils only according to their morphology, e.g. levels of peroxidase activity (Canovi & Campioli, ), but not based on their functionality. Immature cells being functionally competent to form colonies in in vitro conditions might not show the same dynamics as the different mature myeloid subgroups or total white blood cell counts found with flow cytometry.…”

Section: Introductionmentioning

confidence: 99%

Myocardial infarction does not affect circulating haematopoietic stem and progenitor cell self‐renewal ability in a rat model

Kröpfl

Spengler

Frobert

et al. 2017

Experimental Physiology

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Edited by: Kenneth MacLeod New Findings r What is the central question of this study?Although peripheral blood haematopoietic stem and progenitor cells are potentially important in regeneration after acute myocardial infarction, their self-renewal ability in the post-acute phase has not yet been addressed. r What is the main finding and its importance?In rat peripheral blood, we show that myocardial infarction does not negatively affect circulating haematopoietic stem and progenitor cell self-renewal ability 2 weeks after acute infarction, which suggests a constant regenerative potential in the myocardial infarction post-acute phase.Given the importance of peripheral blood haematopoietic stem and progenitor cells (HPCs) in post-acute regeneration after acute myocardial infarction (MI), the aim of the present study was to investigate the number and secondary replating capacity/self-renewal ability of HPCs in peripheral blood before and 2 weeks after MI. In female Lewis inbred rats (n = 9), MI was induced by ligation of the left coronary artery, and another nine underwent sham surgery, without ligation, for control purposes. Myocardial infarction was confirmed by troponin I concentrations 24 h after surgery. Peripheral blood was withdrawn and fractional shortening and ejection fraction of the left ventricle were assessed before (day 0) and 14 days after MI or sham surgery (day 14). After mononuclear cell isolation, primary and secondary functional colony-forming unit granulocyte-macrophage (CFU-GM) assays were performed in order to detect the kinetics of functional HPC colony counts and cell self-renewal ability in vitro. The CFU-GM counts and cell self-renewal ability remained unchanged (P > 0.05) in both groups at day 14, without interaction between groups. In the intervention group, higher day 0 CFU-GM counts showed a relationship to lower fractional shortening on day 14 (ρ = −0.82; P < 0.01). Myocardial infarction did not negatively affect circulating HPC self-renewal ability, which suggests a constant regenerative potential in the post-acute phase. A relationship of cardiac contractile function 14 days after MI with circulating CFU-GM counts on day 0 might imply functional colony count as a predictive factor for outcome after infarction.

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‘Complete blood counts and automated leucocyte differential results obtained by Siemens ADVIA 2120i in 145 cases of acute leukaemia in adults: insights into cellular pathophysiology and differential diagnosis’

Cited by 2 publications

References 9 publications

Large Unstained Cell, Blast Suspect and Delta Neutrophil Index ll Analyzed with Automated Hematology Analyzer as Parameters for the Prediction of Acute Leukemia Relapse

Large Unstained Cell, Blast Suspect and Delta Neutrophil Index ll Analyzed with Automated Hematology Analyzer as Parameters for the Prediction of Acute Leukemia Relapse

Myocardial infarction does not affect circulating haematopoietic stem and progenitor cell self‐renewal ability in a rat model

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